diff --git a/pkg/fanal/image/registry/ecr/ecr.go b/pkg/fanal/image/registry/ecr/ecr.go
index e675ed47afaf..3d644075a218 100644
--- a/pkg/fanal/image/registry/ecr/ecr.go
+++ b/pkg/fanal/image/registry/ecr/ecr.go
@@ -3,6 +3,7 @@ package ecr
 import (
 	"context"
 	"encoding/base64"
+	"regexp"
 	"strings"
 
 	"github.com/aws/aws-sdk-go-v2/aws"
@@ -12,10 +13,9 @@ import (
 	"golang.org/x/xerrors"
 
 	"github.com/aquasecurity/trivy/pkg/fanal/types"
+	"github.com/aquasecurity/trivy/pkg/log"
 )
 
-const ecrURL = "amazonaws.com"
-
 type ecrAPI interface {
 	GetAuthorizationToken(ctx context.Context, params *ecr.GetAuthorizationTokenInput, optFns ...func(*ecr.Options)) (*ecr.GetAuthorizationTokenOutput, error)
 }
@@ -24,24 +24,28 @@ type ECR struct {
 	Client ecrAPI
 }
 
-func getSession(option types.RegistryOptions) (aws.Config, error) {
+func getSession(domain, region string, option types.RegistryOptions) (aws.Config, error) {
 	// create custom credential information if option is valid
 	if option.AWSSecretKey != "" && option.AWSAccessKey != "" && option.AWSRegion != "" {
+		if region != option.AWSRegion {
+			log.Logger.Warnf("The region from AWS_REGION (%s) is being overridden. The region from domain (%s) was used.", option.AWSRegion, domain)
+		}
 		return config.LoadDefaultConfig(
 			context.TODO(),
-			config.WithRegion(option.AWSRegion),
+			config.WithRegion(region),
 			config.WithCredentialsProvider(credentials.NewStaticCredentialsProvider(option.AWSAccessKey, option.AWSSecretKey, option.AWSSessionToken)),
 		)
 	}
-	return config.LoadDefaultConfig(context.TODO())
+	return config.LoadDefaultConfig(context.TODO(), config.WithRegion(region))
 }
 
 func (e *ECR) CheckOptions(domain string, option types.RegistryOptions) error {
-	if !strings.HasSuffix(domain, ecrURL) {
+	region := determineRegion(domain)
+	if region == "" {
 		return xerrors.Errorf("ECR : %w", types.InvalidURLPattern)
 	}
 
-	cfg, err := getSession(option)
+	cfg, err := getSession(domain, region, option)
 	if err != nil {
 		return err
 	}
@@ -51,6 +55,26 @@ func (e *ECR) CheckOptions(domain string, option types.RegistryOptions) error {
 	return nil
 }
 
+// Endpoints take the form
+// <registry-id>.dkr.ecr.<region>.amazonaws.com
+// <registry-id>.dkr.ecr-fips.<region>.amazonaws.com
+// <registry-id>.dkr.ecr.<region>.amazonaws.com.cn
+// <registry-id>.dkr.ecr.<region>.sc2s.sgov.gov
+// <registry-id>.dkr.ecr.<region>.c2s.ic.gov
+// see
+// - https://docs.aws.amazon.com/general/latest/gr/ecr.html
+// - https://docs.amazonaws.cn/en_us/aws/latest/userguide/endpoints-arns.html
+// - https://github.com/boto/botocore/blob/1.34.51/botocore/data/endpoints.json
+var ecrEndpointMatch = regexp.MustCompile(`^[^.]+\.dkr\.ecr(?:-fips)?\.([^.]+)\.(?:amazonaws\.com(?:\.cn)?|sc2s\.sgov\.gov|c2s\.ic\.gov)$`)
+
+func determineRegion(domain string) string {
+	matches := ecrEndpointMatch.FindStringSubmatch(domain)
+	if matches != nil {
+		return matches[1]
+	}
+	return ""
+}
+
 func (e *ECR) GetCredential(ctx context.Context) (username, password string, err error) {
 	input := &ecr.GetAuthorizationTokenInput{}
 	result, err := e.Client.GetAuthorizationToken(ctx, input)
diff --git a/pkg/fanal/image/registry/ecr/ecr_test.go b/pkg/fanal/image/registry/ecr/ecr_test.go
index 63ae1858114c..3a56ffc3ef79 100644
--- a/pkg/fanal/image/registry/ecr/ecr_test.go
+++ b/pkg/fanal/image/registry/ecr/ecr_test.go
@@ -8,21 +8,76 @@ import (
 	"github.com/aws/aws-sdk-go-v2/aws"
 	"github.com/aws/aws-sdk-go-v2/service/ecr"
 	awstypes "github.com/aws/aws-sdk-go-v2/service/ecr/types"
+	"github.com/stretchr/testify/require"
 
 	"github.com/aquasecurity/trivy/pkg/fanal/types"
 )
 
+type testECRClient interface {
+	Options() ecr.Options
+}
+
 func TestCheckOptions(t *testing.T) {
 	var tests = map[string]struct {
-		domain  string
-		wantErr error
+		domain         string
+		expectedRegion string
+		wantErr        error
 	}{
 		"InvalidURL": {
 			domain:  "alpine:3.9",
 			wantErr: types.InvalidURLPattern,
 		},
 		"NoOption": {
-			domain: "xxx.ecr.ap-northeast-1.amazonaws.com",
+			domain:         "xxx.dkr.ecr.ap-northeast-1.amazonaws.com",
+			expectedRegion: "ap-northeast-1",
+		},
+		"region-1": {
+			domain:         "xxx.dkr.ecr.region-1.amazonaws.com",
+			expectedRegion: "region-1",
+		},
+		"region-2": {
+			domain:         "xxx.dkr.ecr.region-2.amazonaws.com",
+			expectedRegion: "region-2",
+		},
+		"fips-region-1": {
+			domain:         "xxx.dkr.ecr-fips.fips-region.amazonaws.com",
+			expectedRegion: "fips-region",
+		},
+		"cn-region-1": {
+			domain:         "xxx.dkr.ecr.region-1.amazonaws.com.cn",
+			expectedRegion: "region-1",
+		},
+		"cn-region-2": {
+			domain:         "xxx.dkr.ecr.region-2.amazonaws.com.cn",
+			expectedRegion: "region-2",
+		},
+		"sc2s-region-1": {
+			domain:         "xxx.dkr.ecr.sc2s-region.sc2s.sgov.gov",
+			expectedRegion: "sc2s-region",
+		},
+		"c2s-region-1": {
+			domain:         "xxx.dkr.ecr.c2s-region.c2s.ic.gov",
+			expectedRegion: "c2s-region",
+		},
+		"invalid-ecr": {
+			domain:  "xxx.dkrecr.region-1.amazonaws.com",
+			wantErr: types.InvalidURLPattern,
+		},
+		"invalid-fips": {
+			domain:  "xxx.dkr.ecrfips.fips-region.amazonaws.com",
+			wantErr: types.InvalidURLPattern,
+		},
+		"invalid-cn": {
+			domain:  "xxx.dkr.ecr.region-2.amazonaws.cn",
+			wantErr: types.InvalidURLPattern,
+		},
+		"invalid-sc2s": {
+			domain:  "xxx.dkr.ecr.sc2s-region.sc2s.sgov",
+			wantErr: types.InvalidURLPattern,
+		},
+		"invalid-cs2": {
+			domain:  "xxx.dkr.ecr.c2s-region.c2s.ic",
+			wantErr: types.InvalidURLPattern,
 		},
 	}
 
@@ -35,6 +90,9 @@ func TestCheckOptions(t *testing.T) {
 			}
 			continue
 		}
+
+		client := a.Client.(testECRClient)
+		require.Equal(t, v.expectedRegion, client.Options().Region)
 	}
 }