[make_msa] ERROR

[oghzzang]

Dear RoseTTAFold Team,

Hi, I'm a user!

I tried to predict a large protein (>2500 amino acids) using RoseTTAFold in Linux server.

But, I got this message.

[log/make_msa.stderr]

...skipping...
- 18:32:30.435 INFO: Output file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-10.id90cov75.a3m

- 18:32:31.159 WARNING: Maximum number 65535 of sequences exceeded in file /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-10.a3m

- 18:32:32.804 INFO: Input file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-10.a3m

- 18:32:32.804 INFO: Output file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-10.id90cov50.a3m

- 18:32:33.536 WARNING: Maximum number 65535 of sequences exceeded in file /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-10.a3m

- 19:21:05.155 INFO: Input file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-6.a3m

- 19:21:05.156 INFO: Output file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-6.id90cov75.a3m

- 19:21:05.882 WARNING: Maximum number 65535 of sequences exceeded in file /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-6.a3m

- 19:21:07.572 INFO: Input file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-6.a3m

- 19:21:07.572 INFO: Output file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-6.id90cov50.a3m

- 19:21:08.304 WARNING: Maximum number 65535 of sequences exceeded in file /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-6.a3m

- 20:06:41.142 INFO: Input file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-3.a3m

- 20:06:41.143 INFO: Output file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-3.id90cov75.a3m

- 20:06:41.944 WARNING: Maximum number 65535 of sequences exceeded in file /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-3.a3m

- 20:06:43.603 INFO: Input file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-3.a3m

- 20:06:43.603 INFO: Output file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-3.id90cov50.a3m

- 20:06:44.375 WARNING: Maximum number 65535 of sequences exceeded in file /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db0.1e-3.a3m

- 21:33:28.849 INFO: Input file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db1.1e-30.a3m

- 21:33:28.862 INFO: Output file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db1.1e-30.id90cov75.a3m

- 21:33:29.606 WARNING: Maximum number 65535 of sequences exceeded in file /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db1.1e-30.a3m

- 21:33:31.181 INFO: Input file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db1.1e-30.a3m

- 21:33:31.181 INFO: Output file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db1.1e-30.id90cov50.a3m

- 21:33:31.899 WARNING: Maximum number 65535 of sequences exceeded in file /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db1.1e-30.a3m

- 01:45:15.929 INFO: Input file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db1.1e-10.a3m

- 01:45:15.929 INFO: Output file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db1.1e-10.id90cov75.a3m

- 01:45:16.768 WARNING: Maximum number 65535 of sequences exceeded in file /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db1.1e-10.a3m

- 01:45:18.302 INFO: Input file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db1.1e-10.a3m

- 01:45:18.302 INFO: Output file = /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db1.1e-10.id90cov50.a3m

- 01:45:19.051 WARNING: Maximum number 65535 of sequences exceeded in file /data7/Programs/RoseTTAFold/results/polq/polq_wild/hhblits/t000_.db1.1e-10.a3m

/data7/Programs/RoseTTAFold/input_prep/make_msa.sh: line 31: 31368 Killed                  $HHBLITS -i $prev_a3m -oa3m $tmp_dir/t000_.db$i.$e.a3m -e $e -v 0 -d ${DATABASES[$i]}

How can solve this problem??

Many thanks,

Oh.

[ShuZishan]

Hello, may I ask if you have solved this problem? I have encountered the same issue.