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109 changes: 109 additions & 0 deletions
109
abr-testing/abr_testing/protocols/test_protocols/tc_biorad_evap_test.py
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| Original file line number | Diff line number | Diff line change |
|---|---|---|
| @@ -0,0 +1,109 @@ | ||
| """Test TC Disposable Lid with BioRad Plate.""" | ||
|
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||
| from opentrons.protocol_api import ( | ||
| ProtocolContext, | ||
| ParameterContext, | ||
| Well, | ||
| Labware, | ||
| InstrumentContext, | ||
| ) | ||
| from typing import List | ||
| from abr_testing.protocols import helpers | ||
| from opentrons.protocol_api.module_contexts import ThermocyclerContext | ||
| from opentrons.hardware_control.modules.types import ThermocyclerStep | ||
|
|
||
| metadata = {"protocolName": "Tough Auto Seal Lid Evaporation Test"} | ||
| requirements = {"robotType": "Flex", "apiLevel": "2.21"} | ||
|
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||
|
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| def add_parameters(parameters: ParameterContext) -> None: | ||
| """Add Parameters.""" | ||
| helpers.create_single_pipette_mount_parameter(parameters) | ||
| helpers.create_tc_lid_deck_riser_parameter(parameters) | ||
| helpers.create_tc_compatible_labware_parameter(parameters) | ||
|
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||
|
|
||
| def _pcr_cycle(thermocycler: ThermocyclerContext) -> None: | ||
| """30x cycles of: 70° for 30s 72° for 30s 95° for 10s.""" | ||
| profile_TAG2: List[ThermocyclerStep] = [ | ||
| {"temperature": 70, "hold_time_seconds": 30}, | ||
| {"temperature": 72, "hold_time_seconds": 30}, | ||
| {"temperature": 95, "hold_time_seconds": 10}, | ||
| ] | ||
| thermocycler.execute_profile( | ||
| steps=profile_TAG2, repetitions=30, block_max_volume=50 | ||
| ) | ||
|
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||
|
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| def _fill_with_liquid_and_measure( | ||
| protocol: ProtocolContext, | ||
| pipette: InstrumentContext, | ||
| reservoir: Labware, | ||
| plate_in_cycler: Labware, | ||
| ) -> None: | ||
| """Fill plate with 10 ul per well.""" | ||
| locations: List[Well] = [ | ||
| plate_in_cycler["A1"], | ||
| plate_in_cycler["A2"], | ||
| plate_in_cycler["A3"], | ||
| plate_in_cycler["A4"], | ||
| plate_in_cycler["A5"], | ||
| plate_in_cycler["A6"], | ||
| plate_in_cycler["A7"], | ||
| plate_in_cycler["A8"], | ||
| plate_in_cycler["A9"], | ||
| plate_in_cycler["A10"], | ||
| plate_in_cycler["A11"], | ||
| plate_in_cycler["A12"], | ||
| ] | ||
| volumes = [10, 10, 10, 10, 10, 10, 10, 10, 10, 10, 10, 10] | ||
| protocol.pause("Weight Armadillo Plate, place on thermocycler") | ||
| # pipette 10uL into Armadillo wells | ||
| source_well: Well = reservoir["A1"] | ||
| pipette.distribute( | ||
| volume=volumes, | ||
| source=source_well, | ||
| dest=locations, | ||
| return_tips=True, | ||
| blow_out=False, | ||
| ) | ||
| protocol.pause("Weight Armadillo Plate, place on thermocycler, put on lid") | ||
|
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||
|
|
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| def run(ctx: ProtocolContext) -> None: | ||
| """Evaporation Test.""" | ||
| pipette_mount = ctx.params.pipette_mount # type: ignore[attr-defined] | ||
| deck_riser = ctx.params.deck_riser # type: ignore[attr-defined] | ||
| labware_tc_compatible = ctx.params.labware_tc_compatible # type: ignore[attr-defined] | ||
| tiprack_50 = ctx.load_labware("opentrons_flex_96_tiprack_50ul", "B2") | ||
| ctx.load_trash_bin("A3") | ||
| tc_mod: ThermocyclerContext = ctx.load_module( | ||
| helpers.tc_str | ||
| ) # type: ignore[assignment] | ||
| plate_in_cycler = tc_mod.load_labware(labware_tc_compatible) | ||
| p50 = ctx.load_instrument("flex_8channel_50", pipette_mount, tip_racks=[tiprack_50]) | ||
| unused_lids = helpers.load_disposable_lids(ctx, 5, ["D2"], deck_riser) | ||
| top_lid = unused_lids[0] | ||
| reservoir = ctx.load_labware("nest_12_reservoir_15ml", "A2") | ||
| tc_mod.open_lid() | ||
| tc_mod.set_block_temperature(4) | ||
| tc_mod.set_lid_temperature(105) | ||
|
|
||
| # hold at 95° for 3 minutes | ||
| profile_TAG: List[ThermocyclerStep] = [{"temperature": 95, "hold_time_minutes": 3}] | ||
| # hold at 72° for 5min | ||
| profile_TAG3: List[ThermocyclerStep] = [{"temperature": 72, "hold_time_minutes": 5}] | ||
| tc_mod.open_lid() | ||
| _fill_with_liquid_and_measure(ctx, p50, reservoir, plate_in_cycler) | ||
| ctx.move_labware(top_lid, plate_in_cycler, use_gripper=True) | ||
| tc_mod.close_lid() | ||
| tc_mod.execute_profile(steps=profile_TAG, repetitions=1, block_max_volume=50) | ||
| _pcr_cycle(tc_mod) | ||
| tc_mod.execute_profile(steps=profile_TAG3, repetitions=1, block_max_volume=50) | ||
| # # # Cool to 4° | ||
| tc_mod.set_block_temperature(4) | ||
| tc_mod.set_lid_temperature(105) | ||
| # Open lid | ||
| tc_mod.open_lid() | ||
| ctx.move_labware(top_lid, "C2", use_gripper=True) | ||
| ctx.move_labware(top_lid, unused_lids[1], use_gripper=True) |
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