diff --git a/abr-testing/abr_testing/protocols/liquid_setups/7_HDQ DNA Bacteria Extraction Liquid Setup.py b/abr-testing/abr_testing/protocols/liquid_setups/7_HDQ DNA Bacteria Extraction Liquid Setup.py index 410e46fd9bb..4addbd5c7e8 100644 --- a/abr-testing/abr_testing/protocols/liquid_setups/7_HDQ DNA Bacteria Extraction Liquid Setup.py +++ b/abr-testing/abr_testing/protocols/liquid_setups/7_HDQ DNA Bacteria Extraction Liquid Setup.py @@ -35,15 +35,23 @@ def run(protocol: protocol_api.ProtocolContext) -> None: res1 = protocol.load_labware("nest_12_reservoir_15ml", "D2", "reagent reservoir 1") # Label Reservoirs well1 = res1["A1"].top() + well2 = res1["A2"].top() well3 = res1["A3"].top() well4 = res1["A4"].top() + well5 = res1["A5"].top() + well6 = res1["A6"].top() well7 = res1["A7"].top() + well8 = res1["A8"].top() + well9 = res1["A9"].top() well10 = res1["A10"].top() - + well11 = res1["A11"].top() + well12 = res1["A12"].top() # Volumes wash = 600 - al_and_pk = 468 - beads_and_binding = 552 + binding = 320 + beads = 230 + pk = 230 + lysis = 230 # Sample Plate p1000.transfer( @@ -65,9 +73,41 @@ def run(protocol: protocol_api.ProtocolContext) -> None: ) # Res 1 p1000.transfer( - volume=[beads_and_binding, al_and_pk, wash, wash, wash], + volume=[ + binding, + beads, + binding, + beads, + lysis, + pk, + wash, + wash, + wash, + wash, + wash, + wash, + wash, + wash, + wash, + ], source=source_reservoir["A1"].bottom(z=0.5), - dest=[well1, well3, well4, well7, well10], + dest=[ + well1, + well1, + well2, + well2, + well3, + well3, + well4, + well5, + well6, + well7, + well8, + well9, + well10, + well11, + well12, + ], blowout=True, blowout_location="source well", trash=False,