chore(Release): 2024-03-07

data/data/fields.csv

@@ -438,7 +438,7 @@ empty_vial_well,3
 end_col,1
 end_column,1
 end_repair,1
-engage_height,19
+engage_height,20
 engage_offset,4
 engage_time,17
 enz_asp_rate,1
@@ -575,7 +575,7 @@ init_vol,3
 init_vol_50,1
 init_vol_bca,1
 init_vol_bsa,1
-init_vol_buff,2
+init_vol_buff,1
 init_vol_buff1,1
 init_vol_buff2,1
 init_vol_buff3,1
@@ -586,7 +586,7 @@ init_vol_standard,1
 init_vols_csv,1
 initial_denaturation_cycles,1
 initial_vol,1
-input_csv,23
+input_csv,22
 input_file,10
 input_file2,6
 input_labware,1
@@ -643,7 +643,6 @@ labware_collect_plate,1
 labware_cultureplate,1
 labware_deep_well,1
 labware_dest,2
-labware_dna_plate,1
 labware_dna_sample_plate,1
 labware_elisa_plate,3
 labware_firstrna,1
@@ -655,7 +654,7 @@ labware_p300_tips,1
 labware_parent,2
 labware_patient_samples,1
 labware_pcr,1
-labware_pcr_plate,11
+labware_pcr_plate,10
 labware_plate,1
 labware_plates,9
 labware_pool,1
@@ -891,6 +890,7 @@ num_col,10
 num_cols,3
 num_columns,1
 num_curves,1
+num_cycles,1
 num_daughter_plates,1
 num_drug_plates,1
 num_extracts,1
@@ -991,7 +991,7 @@ p1kmnt,1
 p1n,1
 p1num,1
 p20_blowout_height,1
-p20_mount,137
+p20_mount,136
 p20_multi_mount,3
 p20_rate,2
 p20_reservoir_height,1
@@ -1008,7 +1008,7 @@ p2num,1
 p300_gen,1
 p300_mixing_height,1
 p300_mnt,3
-p300_mount,160
+p300_mount,159
 p300_mount_1,1
 p300_multi_mount,7
 p300_rate,1
@@ -1450,7 +1450,7 @@ te_well,2
 temp,2
 temp_gen,1
 temp_lysate,1
-temp_mod,3
+temp_mod,2
 temp_mod_lname,2
 temp_mod_on,2
 temp_mod_s1_lname,1
@@ -1471,7 +1471,7 @@ tempblock,1
 tempdeck_gen,1
 tempdeck_name,1
 tempdeck_slot,1
-temperature,13
+temperature,12
 temperature1,1
 temperature2,1
 tempgen,1
@@ -1554,7 +1554,7 @@ transfer2,1
 transfer_csv,21
 transfer_scheme,2
 transfer_to_storage,1
-transfer_vol,10
+transfer_vol,9
 transfer_volume,3
 transfercsv,2
 transfervol,1
@@ -1614,6 +1614,8 @@ use_filter_tips_left,2
 use_filter_tips_right,2
 use_middle_2,2
 use_nacl,1
+use_p20,1
+use_p300,1
 use_single_source,1
 use_temp,1
 use_tuberack_a,1

protoBuilds/04711e/04711e.ot2.apiv2.py.json

@@ -1,5 +1,5 @@
 {
-    "content": "# flake8: noqa\n\nimport math\n\nmetadata = {\n    'protocolName': 'PCR Prep',\n    'author': 'Rami Farawi <[email protected]>',\n    'source': 'Custom Protocol Request',\n    'apiLevel': '2.13'\n}\n\n\ndef run(ctx):\n\n    [num_samp,\n      p300_mount, m20_mount] = get_values(  # noqa: F821\n      \"num_samp\", \"p300_mount\", \"m20_mount\")\n\n    # num_samp = 16\n    # p300_mount = 'left'\n    # m20_mount = 'right'\n\n    # labware\n    tuberack = ctx.load_labware('opentrons_24_tuberack_2000ul', 4)\n\n    source_plate = ctx.load_labware('pcrplate_96_wellplate_200ul', 1)\n    middle_plate = ctx.load_labware('pcrplate_96_wellplate_200ul', 2)\n    final_plate = ctx.load_labware('pcrplate_96_wellplate_200ul', 3)\n\n    tip300 = [ctx.load_labware('opentrons_96_tiprack_300ul', slot)\n              for slot in [6]]\n    tip20 = [ctx.load_labware('opentrons_96_filtertiprack_20ul', slot)\n               for slot in [10, 11]]\n\n    # pipettes\n    p300 = ctx.load_instrument('p300_single_gen2', p300_mount,\n                               tip_racks=tip300)\n    m20 = ctx.load_instrument('p20_multi_gen2', m20_mount,\n                                tip_racks=tip20)\n\n    # mapping\n    mmx = tuberack['A1']\n    primer = tuberack['A2']\n    final_mmx = tuberack['A3']\n    num_col = math.ceil(num_samp/8)\n\n    # protocol\n    ctx.comment('\\n---------------MAKING MASTERMIX----------------\\n\\n')\n    mmx_vol = 9.1\n    primer_vol = 5.57\n\n    p300.pick_up_tip()\n    p300.transfer(mmx_vol*num_samp*1.15, mmx, final_mmx, new_tip='never')\n    p300.drop_tip()\n\n    total_vol = num_samp*(primer_vol+mmx_vol)\n    p300.pick_up_tip()\n    p300.transfer(primer_vol*num_samp*1.15, primer, final_mmx, new_tip='never')\n    p300.mix(20, total_vol if total_vol < 300 else 300, final_mmx)\n    p300.drop_tip()\n\n    vol_per_col_well = (mmx_vol+primer_vol)*12*1.1\n    p300.pick_up_tip()\n    for well in middle_plate.columns()[0]:\n        p300.aspirate(vol_per_col_well, final_mmx)\n        p300.dispense(vol_per_col_well, well)\n    p300.drop_tip()\n\n    ctx.comment('\\n---------------DISTRIBUTING MASTERMIX----------------\\n\\n')\n    m20.pick_up_tip()\n    for col in final_plate.rows()[0][:num_col]:\n        m20.aspirate(14.7, middle_plate.rows()[0][0])\n        m20.dispense(14.7, col)\n    m20.drop_tip()\n\n    ctx.comment('\\n---------------DISTRIBUTING SAMPLE----------------\\n\\n')\n    for s, d in zip(source_plate.rows()[0][:num_col], final_plate.rows()[0]):\n        m20.pick_up_tip()\n        m20.aspirate(4, s)\n        m20.dispense(4, d)\n        m20.mix(5, 15, d)\n        m20.drop_tip()\n",
+    "content": "# flake8: noqa\n\nimport math\n\nmetadata = {\n    'protocolName': 'PCR Prep',\n    'author': 'Rami Farawi <[email protected]>',\n    'source': 'Custom Protocol Request',\n    'apiLevel': '2.13'\n}\n\n\ndef run(ctx):\n\n    [num_samp,\n      p300_mount, m20_mount] = get_values(  # noqa: F821\n      \"num_samp\", \"p300_mount\", \"m20_mount\")\n\n    # num_samp = 16\n    # p300_mount = 'left'\n    # m20_mount = 'right'\n\n    # labware\n    tuberack = ctx.load_labware('opentrons_24_tuberack_2000ul', 4)\n\n    source_plate = ctx.load_labware('pcrplate_96_wellplate_200ul', 1)\n    middle_plate = ctx.load_labware('pcrplate_96_wellplate_200ul', 2)\n    final_plate = ctx.load_labware('pcrplate_96_wellplate_200ul', 3)\n\n    tip300 = [ctx.load_labware('opentrons_96_tiprack_300ul', slot)\n              for slot in [6]]\n    tip20 = [ctx.load_labware('opentrons_96_filtertiprack_20ul', slot)\n               for slot in [10, 11]]\n\n    # pipettes\n    p300 = ctx.load_instrument('p300_single_gen2', p300_mount,\n                               tip_racks=tip300)\n    m20 = ctx.load_instrument('p20_multi_gen2', m20_mount,\n                                tip_racks=tip20)\n\n    # mapping\n    mmx = tuberack['A1']\n    primer = tuberack['A2']\n    final_mmx = tuberack['A3']\n    num_col = math.ceil(num_samp/8)\n\n    # protocol\n    ctx.comment('\\n---------------MAKING MASTERMIX----------------\\n\\n')\n    mmx_vol = 9.1\n    primer_vol = 5.57\n\n    p300.pick_up_tip()\n    p300.transfer(mmx_vol*num_samp*1.15, mmx, final_mmx, new_tip='never')\n    p300.drop_tip()\n\n    total_vol = num_samp*(primer_vol+mmx_vol)\n    p300.pick_up_tip()\n    p300.transfer(primer_vol*num_samp*1.15, primer, final_mmx, new_tip='never')\n    for _ in range(20):\n        p300.aspirate(total_vol if total_vol < 300 else 300, final_mmx)\n        p300.dispense(total_vol if total_vol < 300 else 300, final_mmx.top(z=-3))\n    p300.mix(20, total_vol if total_vol < 300 else 300, final_mmx)\n    p300.drop_tip()\n\n    vol_per_col_well = (mmx_vol+primer_vol)*12*1.1\n    p300.pick_up_tip()\n    for well in middle_plate.columns()[0]:\n        p300.aspirate(vol_per_col_well, final_mmx)\n        p300.dispense(vol_per_col_well, well)\n    p300.drop_tip()\n\n    ctx.comment('\\n---------------DISTRIBUTING MASTERMIX----------------\\n\\n')\n    m20.pick_up_tip()\n    for col in final_plate.rows()[0][:num_col]:\n        m20.aspirate(14.7, middle_plate.rows()[0][0])\n        m20.dispense(14.7, col)\n    m20.drop_tip()\n\n    ctx.comment('\\n---------------DISTRIBUTING SAMPLE----------------\\n\\n')\n    for s, d in zip(source_plate.rows()[0][:num_col], final_plate.rows()[0]):\n        m20.pick_up_tip()\n        m20.aspirate(4, s)\n        m20.dispense(4, d)\n        m20.mix(5, 15, d)\n        m20.drop_tip()\n",
     "custom_labware_defs": [
         {
             "brand": {