diff --git a/protocols/0ff0c3/0ff0c3.ot2.apiv2.py b/protocols/0ff0c3/0ff0c3.ot2.apiv2.py index b2f3bcf0a..e00078004 100644 --- a/protocols/0ff0c3/0ff0c3.ot2.apiv2.py +++ b/protocols/0ff0c3/0ff0c3.ot2.apiv2.py @@ -198,15 +198,20 @@ def slow_tip_withdrawal(current_pipette, well_location, to_center=False): pick_up(m300) m300.aspirate(50, elution_buff) m300.dispense(50, col) - m300.mix(15, 50, col) + + for i in range(20): + m300.aspirate(50, col.bottom(0.2), rate=2) + m300.dispense(50, col.bottom(2), rate=2) + + #m300.mix(15, 50, col) m300.drop_tip() - - mag_mod.engage(height_from_base=4.0) - ctx.delay(minutes=5) + #May need to replace this with a for loop for the mixing + mag_mod.engage(height_from_base=3.5) + #ctx.delay(minutes=5) for s, d in zip(samples, destination): pick_up(m300) - m300.aspirate(50, s.bottom(0.4), rate=0.1) + m300.aspirate(50, s.bottom(0.1), rate=0.1) m300.dispense(50, d) m300.drop_tip()