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@@ -19,6 +19,8 @@ $ apt-get install moreutils
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```
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<br/>
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## In case of non-unique FASTA IDs -> make them unique
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### 🏷 one possible way as follows:
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```
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$ awk '/^>/ {$0=$0"_Seq"++count[$0]}1' input.fasta > output.fasta
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```
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## 📓 Shiny script: MIR_loci_overlaps.R
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```
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inpfn.fasta - input file (fasta format)
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```
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# 📝 Useful links and code lines
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## :wrench: convert line breaks from DOS to Unix format (dos2unix) and vice versa (unix2dos)
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## :clipboard: Obtaining materials from the GitHub
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i) use wget to pull down the ```raw``` file
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- use wget to pull down the ```raw``` file
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```
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$ wget https://raw.githubusercontent.com/username/reponame/path/to/file
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```
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ii) use git clone to pull the complete repository (prerequisites: user with ```sudo``` privileges)
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- use git clone to pull the complete repository (prerequisites: user with ```sudo``` privileges)
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```
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$ sudo apt update
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$ sudo apt install git
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$ git clone https://github.com/username/reponame.git
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```
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## :clipboard: Install Perl on Ubuntu-like Linux OS
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i) Install Perl on Ubuntu-like Linux OS (prerequisites: user with ```sudo``` privileges)
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- Install Perl on Ubuntu-like Linux OS (prerequisites: user with ```sudo``` privileges)
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```
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$ sudo apt update
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$ sudo apt-get install perl
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```
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More details for installing Perl can be found at [perl.org](www.perl.org/get.html#unix_like)
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ii) Use [CPAN](www.cpan.org) (‘Comprehensive Perl Archive Network’) to install Perl modules
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- Use [CPAN](www.cpan.org) (‘Comprehensive Perl Archive Network’) to install Perl modules
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iii) Install different version of Perl
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- Install different version of Perl
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```
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$ sudo cpan App::perlbrew
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$ perlbrew init
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## :clipboard: Install R on Ubuntu-like Linux OS
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Install R on Ubuntu-like Linux OS (prerequisites: user with sudo privileges) with add-apt-repository (adapt values in the square brackets and delete the brackets)
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i) Import the repository public key
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- Import the repository public key
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```
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$ apt-key adv --keyserver [from this location or server] --recv-keys [retrieve key(s)]
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```
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ii) Add the CRAN repository to your system sources’ list
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- Add the CRAN repository to your system sources’ list
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```
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$ sudo add-apt-repository ‘deb https://cloud.r-project.org/bin/linux/ubuntu [type appropriate selection from https://cloud.r-project.org/bin/linux/ubuntu/]’
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```
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iii) Install the complete R system
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- Install the complete R system
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```
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$ sudo apt-get update
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$ sudo apt install r-base
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# Installing pip for Python 3
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$ sudo apt update
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$ sudo apt install python3-pip
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# Installing pip for Python 2
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$ sudo apt update
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$ sudo apt install python2
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[Wine](https://www.winehq.org/)
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<br/>
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## :notebook_with_decorative_cover: Shiny and R Markdown
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[R Markdown introduction](https://rmarkdown.rstudio.com/lesson-1.html)
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## :green_book: FAIR data
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[The FAIR Guiding Principles for scientific data management and stewardship](https://www.nature.com/articles/sdata201618)

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