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pointmatch.m
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pointmatch.m
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function exitcode = pointmatch(tile1, tile2, acqusitionfolder1, acqusitionfolder2, output_folder_name, pixshift, ch, maxnumofdesc, exitcode) %#ok<INUSL>
% Front-end function to do z point-matching in the Patrick pipeline. In this
% version, the ch argument is vestigial---it is completely ignored
% Deal with arguments
if ~exist('pixshift', 'var') || isempty(pixshift) ,
pixshift = '[0 0 0]' ;
end
if ~exist('maxnumofdesc', 'var') || isempty(maxnumofdesc) ,
maxnumofdesc=1e3 ;
end
if ~exist('exitcode', 'var') || isempty(exitcode) ,
exitcode = 0 ;
end
% Eval args that are strings that (hopefully) represent numeric arrays
if ischar(pixshift)
pixshift = eval(pixshift);
end
if ischar(maxnumofdesc)
maxnumofdesc=str2double(maxnumofdesc);
end
if ischar(exitcode)
exitcode=str2double(exitcode);
end
% Read in stuff from input files
scopefile1 = readScopeFile(acqusitionfolder1);
scopefile2 = readScopeFile(acqusitionfolder2);
X_for_all_channels = zeros(0,3) ;
Y_for_all_channels = zeros(0,3) ;
for channel_index = 0:1 ,
desc1 = readDesc(tile1, channel_index) ;
desc2 = readDesc(tile2, channel_index) ;
% Call the function that does the real work
[paireddescriptor, iadj] = pointmatch_core(desc1, desc2, scopefile1, scopefile2, pixshift, maxnumofdesc) ;
% Write the main output file
tag = 'XYZ';
axis_letter = tag(iadj) ;
output_file_leaf_name = sprintf('channel-%d-match-%s.mat', channel_index, axis_letter) ;
output_file_name = fullfile(output_folder_name, output_file_leaf_name) ;
if exist(output_file_name,'file')
unix(sprintf('rm -f %s',output_file_name)) ;
end
save(output_file_name,'paireddescriptor','scopefile1','scopefile2')
system(sprintf('chmod g+rw %s',output_file_name));
% Save points for making the thumbnail
X_for_all_channels = vertcat(X_for_all_channels, paireddescriptor.X) ; %#ok<AGROW>
Y_for_all_channels = vertcat(Y_for_all_channels, paireddescriptor.Y) ; %#ok<AGROW>
end
% Synthesize and write a thumbnail image file
% x:R, y:G, z:B
if isempty(X_for_all_channels) ,
col = [0 0 0] ; % black as night
else
col = median(Y_for_all_channels-X_for_all_channels,1)+128;
end
col = max(min(col,255),0);
outpng = zeros(105,89,3);
outpng(:,:,1) = col(1);
outpng(:,:,2) = col(2);
outpng(:,:,3) = col(3);
thumbnail_image_file_name = fullfile(output_folder_name,'Thumbs.png') ;
if exist(thumbnail_image_file_name,'file')
system(sprintf('rm -f %s',thumbnail_image_file_name)) ;
end
imwrite(outpng,thumbnail_image_file_name)
system(sprintf('chmod g+rw %s',thumbnail_image_file_name));
end