Repeated failures with SRA HiSeq 2000 data from museum specimen #293
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ResidentialDragonSlayer
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I am having trouble assembling an animal mitochondrion from a GenBank SRA dataset. GetOrganelle is not throwing any errors, but disentangling consistently fails. Having looked at the FAQ on incomplete results, I have adjusted parameters as suggested, but it still didn't work.
I have also tried assembling with SPAdes and disentangling with GetOrganelle, which also didn't work.
Any ideas on what I could try? It's been suggested that my issues are occurring because this is an older type of data. I'm also going to include the FastQC in case the issue is the raw data quality, rather than something with GetOrganelle.
Thank you!
get_org.log.txt
fastqc.zip
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