animal_mt: Warning: No paired reads found!? ends with incomplete assembly #290
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I am trying to assemble a mitochondrial genome using data from GenBank's SRA. Initially the file was interleaved, but when I failed to figure out how to run GetOrganelle on interleaved data I separated it into paired files. I have tried twice with slightly different parameters (second time I set Format of initial fastq files: Thank you! |
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The The disentangling failure is a classic problem and you may first check https://github.com/Kinggerm/GetOrganelle/wiki/FAQ#what-should-i-do-with-incomplete-resultbroken-assembly-graph |
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The
No paired reads found
issue is probably because that the data does not contain pairing information or GetOrganelle did not recognize it well. Anyway, it is not a big issue here and is not the reason for disentangling failure.The disentangling failure is a classic problem and you may first check https://github.com/Kinggerm/GetOrganelle/wiki/FAQ#what-should-i-do-with-incomplete-resultbroken-assembly-graph