nf_bisulfite_scNMT

#!/usr/bin/env nextflow
nextflow.enable.dsl=2

// Last modified 14 October 2020

params.outdir = "."
params.genome = ""
params.verbose = false
params.single_end = false  // default mode is auto-detect. NOTE: params are automatically handed over 

params.fastqc_args = ''
params.fastq_screen_args = ''
params.trim_galore_args = ''
params.bismark_args = ''
params.deduplicate_bismark_args = ''
params.bismark_methylation_extractor_args = ''
params.coverage2cytosine_args = ''
params.bismark2summary_args = ''
params.bismark2report_args= ''
params.multiqc_args = ''

params.help = false
// Show help message and exit
if (params.help){
    helpMessage()
    exit 0
}

params.list_genomes = false;
if (params.list_genomes){
    println ("[WORKLFOW] List genomes selected")
}

if (params.verbose){
    println ("[WORKFLOW] FASTQC ARGS: "           + params.fastqc_args)
    println ("[WORKFLOW] FASTQ SCREEN ARGS ARE: " + params.fastq_screen_args)
    println ("[WORKFLOW] TRIM GALORE ARGS: "      + params.trim_galore_args)
    println ("[WORKFLOW] BISMARK ARGS ARE: "      + params.bismark_args)
    println ("[WORKFLOW] BISMARK DEDUPLICATION ARGS ARE: "         + params.deduplicate_bismark_args)
    println ("[WORKFLOW] BISMARK METHYLATION EXTRACTOR ARGS ARE: " + params.bismark_methylation_extractor_args)
    println ("[WORKFLOW] BISMARK COVERAGE2CYTOSINE ARGS ARE: "     + params.coverage2cytosine_args)
}

include { makeFilesChannel; getFileBaseNames }         from './nf_modules/files.mod.nf'
include { getGenome }                                  from './nf_modules/genomes.mod.nf'
include { listGenomes }                                from './nf_modules/genomes.mod.nf'

if (params.list_genomes){
    listGenomes()  // this lists all available genomes, and exits
}

genome = getGenome(params.genome)

include { FASTQC }                                     from './nf_modules/fastqc.mod.nf'                        
include { FASTQC as FASTQC2 }                          from './nf_modules/fastqc.mod.nf'

include { FASTQ_SCREEN }                               from './nf_modules/fastq_screen.mod.nf' params(bisulfite: '--bisulfite')

// Adding singlecell flag for scBS-seq processing
include { TRIM_GALORE }                                from './nf_modules/trim_galore.mod.nf'  params(singlecell: true)

// Adding singlecell flag for scBS-seq processing
include { BISMARK }                                    from './nf_modules/bismark.mod.nf' params(genome: genome, singlecell: true)
include { BISMARK_DEDUPLICATION }                      from './nf_modules/bismark_deduplication.mod.nf'
include { BISMARK2REPORT }                             from './nf_modules/bismark2report.mod.nf'
include { BISMARK2SUMMARY }                            from './nf_modules/bismark2summary.mod.nf'

// Adding singlecell flag for scBS-seq processing
include { BISMARK_METHYLATION_EXTRACTOR }                from './nf_modules/bismark_methylation_extractor.mod.nf'   params(singlecell: true, nonCG: true)
include { COVERAGE2CYTOSINE }                          from './nf_modules/coverage2cytosine.mod.nf' params(nome: true, genome: genome)
include { MULTIQC }                                     from './nf_modules/multiqc.mod.nf' 

file_ch = makeFilesChannel(args)

workflow {

    main:
        FASTQC                        (file_ch, params.outdir, params.fastqc_args, params.verbose)
        TRIM_GALORE                   (file_ch, params.outdir, params.trim_galore_args, params.verbose)
        FASTQ_SCREEN                  (TRIM_GALORE.out.reads, params.outdir, params.fastq_screen_args, params.verbose)
        FASTQC2                       (TRIM_GALORE.out.reads, params.outdir, params.fastqc_args, params.verbose)
        BISMARK                       (TRIM_GALORE.out.reads, params.outdir, params.bismark_args, params.verbose)
        BISMARK_DEDUPLICATION         (BISMARK.out.bam, params.outdir, params.deduplicate_bismark_args, params.verbose)
        BISMARK_METHYLATION_EXTRACTOR (BISMARK_DEDUPLICATION.out.bam, params.outdir, params.bismark_methylation_extractor_args, params.verbose)
        COVERAGE2CYTOSINE             (BISMARK_METHYLATION_EXTRACTOR.out.coverage, params.outdir, params.coverage2cytosine_args, params.verbose)  
        // merging channels for MultiQC
        multiqc_ch = FASTQC.out.report.mix(
            TRIM_GALORE.out.report,
            FASTQ_SCREEN.out.report.ifEmpty([]),
            FASTQC2.out.report.ifEmpty([]),
            BISMARK.out.report.ifEmpty([]),
            BISMARK_DEDUPLICATION.out.report.ifEmpty([]),
            BISMARK_METHYLATION_EXTRACTOR.out.report.ifEmpty([]),
            BISMARK_METHYLATION_EXTRACTOR.out.mbias.ifEmpty([]),
        ).collect()

        bismark_report_ch = BISMARK.out.bam.mix(
            BISMARK.out.report,
            BISMARK_DEDUPLICATION.out.report.ifEmpty([]),
            BISMARK_METHYLATION_EXTRACTOR.out.report.ifEmpty([]),
            BISMARK_METHYLATION_EXTRACTOR.out.mbias.ifEmpty([]),
        ).collect()

        // bismark_report_ch.subscribe {  println "Got: $it"  }

        BISMARK2REPORT                   (bismark_report_ch, params.outdir, params.bismark2report_args, params.verbose)
        BISMARK2SUMMARY                  (bismark_report_ch, params.outdir, params.bismark2summary_args, params.verbose)
        MULTIQC                          (multiqc_ch, params.outdir, params.multiqc_args, params.verbose)
}

// Since workflows with very long command lines tend to fail to get rendered at all, I was experimenting with a
// minimal execution summary report so we at least know what the working directory was...
workflow.onComplete {

    def msg = """\
        Pipeline execution summary
        ---------------------------
        Completed at: ${workflow.complete}
        Duration    : ${workflow.duration}
        Success     : ${workflow.success}
        workDir     : ${workflow.workDir}
        exit status : ${workflow.exitStatus}
        """
    .stripIndent()

    sendMail(to: "${workflow.userName}@babraham.ac.uk", subject: 'Minimal pipeline execution report', body: msg)
}

def helpMessage() {
 
    log.info"""
    >>


    SYNOPSIS:

    This workflow runs an entire scNMT (single-cell nucleosome, methylation and transcription sequencing) processing
    pipeline on FastQ files (see also https://www.nature.com/articles/s41467-018-03149-4 for more details). This
    includes QC, quality-/adapter trimming, contamination QC (post-trimming), alignments to a genome using Bismark,
    deduplication, methylation extraction, coverage file generation (in --CX mode), NOMe-seq filtering, and finally
    generates an aggregate MultiQC report. Like for the scBS-seq procedure, the scNMT workflow is usually carried out
    in '--single_end' mode, even for paired-end libraries. Here is a graphical representation of the workflow:

    --- FastQC
    --- Trim Galore
        |
        --- FastQ Screen
        --- FastQC
        --- Bismark 
            |
            --- Bismark deduplication
                |
                --- Bismark methylation extraction  
                  |
                  --- Bismark coverage2cytosine           
    --- bismark2report*
    --- bismark2summary*
    --- MultiQC*
        
    * These steps run only once ALL other jobs have completed.
     
    By default all these steps are submitted as jobs to the Babraham stone compute cluster.

    The involved tools are run in the following way:
    ------------------------------------------------
    FastQC:                           defaults (-q)
    FastQ Screen:                     '--bisulfite'
    Trim Galore:                      defaults (adapter auto-detection); '--clip_r1 6 (--clip_r2 6)'
    Bismark:                          '--non_directional'
    Bismark deduplication:            defaults
    Bismark methylation extraction:   '--bedGraph --buffer 10G --parallel 4 --CX'                  
    Bismark coverage2cytosine:        '--nome'

    To add additional parameters to any of the programs, consider supplying tool-specific arguments (see --toolname_args="..." below).


                ==============================================================================================================


    USAGE:
    
    nf_bisulfite_scNMT [options] --genome <genomeID> --single_end (recommended!) <input files>
    
    Mandatory arguments:
    ====================

      <input files>                   List of input files, e.g. '*fastq.gz' or '*fq.gz'. Files are automatically processed as
                                      single-end (SE) or paired end (PE) files (if file pairs share the same base-name, and differ only
                                      by a read number, e.g. 'base_name_R1.fastq.gz' and 'base_name_R2.fastq.gz' (or R3, R4). For
                                      PE files, only Read 1 is run through FastQ Screen (as typically R1 and R2 produce nearly identical
                                      contamination profiles). To run PE files in single-end mode, please see '--single_end' below.

      --genome [str]                  Genome build ID to be used for the alignment, e.g. GRCh38 (latest human genome) or GRCm38
                                      (latest mouse genome build). To list all available genomes, see '--list_genomes' below.


    Tool-specific options:
    ======================

    For all following options, please note that the format: ="your options" needs to be strictly adhered to in order to work correctly.

      --fastqc_args="[str]"           This option can take any number of options that are compatible with FastQC to modify its default
                                      behaviour. For more detailed information on available options please refer to the FastQC documentation,
                                      or run 'fastqc --help' on the command line. As an example, to run FastQC without grouping of bases when
                                      reads are >50bp and use a specific file with non-default adapter sequences, use: 
                                      ' --fastqc_args="--nogroup --adapters ./non_default_adapter_file.txt" '. [Default: None]

      --fastq_screen_args="[str]"     This option can take any number of options that are compatible with FastQ Screen to modify its
                                      default behaviour. For more detailed information on available options please refer to the FastQ Screen
                                      documentation, or run 'fastq_screen --help' on the command line. For instance, to process a bisulfite
                                      converted library with fairly relaxed parameters, you could use: 
                                      ' --fastq_screen_args="--bisulfite --score_min L,0,-0.6" '. [Default: None]

      --trim_galore_args="[str]"      This option can take any number of options that are compatible with Trim Galore to modify its
                                      default trimming behaviour. For more detailed information on available options please refer
                                      to the Trim Galore User Guide, or run 'trim_galore --help' on the command line. As an example, to trim
                                      off the first 10bp from the 5' of R1 and 5bp of R2, use: 
                                      ' --trim_galore_args="--clip_r1 10 --clip_r2 5" '. [Default: None]

      --bismark_args="[str]"          This option can take any number of options that are compatible with Bismark to modify its
                                      default mapping behaviour. For more detailed information on available options please refer
                                      to the Bismark User Guide, or run 'bismark --help' on the comamnd line. As an example, to run somewhat
                                      relaxed alignments, use: ' --bismark_args="--score_min L,0,-0.4" '. [Default: None]

      --bismark_deduplication_args="[str]"           This option can take any number of options that are compatible with deduplicate_bismark
                                      to modify its default deduplication behaviour. For more detailed information on available options please
                                      refer to the Bismark User Guide, or run 'deduplicate_bismark --help'. As an example, to take UMI sequences
                                      into account, use: ' --bismark_deduplication_args="--barcode" '. [Default: None]

      --bismark_methylation_extractor_args="[str]"   This option can take any number of options that are compatible with bismark_methylation_extractor
                                      to modify its default exctraction behaviour. For more detailed information on available options please refer
                                      to the Bismark User Guide, or run 'bismark_methylation_extractor --help' on the command line. As an example,
                                      to run the extraction and coverage file generation for a genome containing thousands of scaffolds
                                      or contigs, use: ' --bismark_methylation_extractor_args="--gazillion" '. [Default: None]

      --coverage2cytosine_args="[str]"               This option can take any number of options that are compatible with Bismark's coverage2cytosine
                                      to modify its default behaviour. For more detailed information on available options please refer
                                      to the Bismark User Guide, or run 'coverage2cytosine --help' on the comamnd line. As an example, to merge
                                      adjacent cytosines in CpG context into CpG dinucleotides, use: 
                                      ' --coverage2cytosine_args="--merge_CpGs" '. [Default: None]


    Other options:
    ==============

      --outdir [str]                  Path to the output directory. [Default: current working directory]

      --list_genomes                  List all genome builds that are currently available to choose from. To see this list
                                      of available genomes with more detailed information about paths and indexes, run
                                      the command as '--list_genomes --verbose'
    
      --single_end                    Force files of a read pair to be treated as single-end files. [Default: auto-detect]
      
      --verbose                       More verbose status messages. [Default: OFF]
      --help                          Displays this help message and exits.

    Workflow options:
    =================

    Please note the single '-' hyphen for the following options!

      -resume                         If a pipeline workflow has been interrupted or stopped (e.g. by accidentally closing a laptop),
                                      this option will attempt to resume the workflow at the point it got interrupted by using
                                      Nextflow's caching mechanism. This may save a lot of time.

      -bg                             Sends the entire workflow into the background, thus disconnecting it from the terminal session.
                                      This option launches a daemon process (which will keep running on the headnode) that watches over
                                      your workflow, and submits new jobs to the SLURM queue as required. Use this option for big pipeline
                                      jobs, or whenever you do not want to watch the status progress yourself. Upon completion, the
                                      pipeline will send you an email with the job details. This option is HIGHLY RECOMMENDED!

      -process.executor=local         Temporarily changes where the workflow is executed to the 'local' machine. See also Nextflow config
                                      file for more details. [Default: slurm] 
    

    <<
    """.stripIndent()

}