@@ -15,42 +15,42 @@ Download the latest POGENOM distribution from https://github.com/EnvGen/POGENOM/
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Either:
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- ` perl pogenom.pl --vcf_file VCF_FILE --out OUTPUT_FILES_PREFIX --genome_size GENOME_SIZE [-h ] `
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+ ` perl pogenom.pl --vcf_file VCF_FILE --out OUTPUT_FILES_PREFIX --genome_size GENOME_SIZE [--help ] `
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Or:
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- ` perl pogenom.pl -vcf_file VCF_FILE --out OUTPUT_FILES_PREFIX --gff_file GFF_FILE [-h ] `
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+ ` perl pogenom.pl -vcf_file VCF_FILE --out OUTPUT_FILES_PREFIX --gff_file GFF_FILE [--help ] `
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### Required arguments
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- ` --vcf_file VCF_FILE ` Specify vcf file with data from a single or multiple samples.
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+ ` --vcf_file VCF_FILE ` Specify vcf file with data from a single or multiple samples.
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- ` --out OUTPUT_FILES_PREFIX ` Specify the prefix of the output file name(s) (overwrites existing files with same names).
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+ ` --out OUTPUT_FILES_PREFIX ` Specify the prefix of the output file name(s) (overwrites existing files with same names).
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- ` --genome_size GENOME_SIZE ` Specify genome size (in bp; integer). Not required if ` --gff_file ` is given.
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+ ` --genome_size GENOME_SIZE ` Specify genome size (in bp; integer). Not required if ` --gff_file ` is given.
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### Optional arguments
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- ` --gff_file GFF_FILE ` Specify gff file. Either this or ` --genome_size ` must be given.
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+ ` --gff_file GFF_FILE ` Specify gff file. Either this or ` --genome_size ` must be given.
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` --genetic_code_file GENETIC_CODE_FILE ` Specify genetic code file. E.g. ` standard_genetic_code.txt ` in the POGENOM distribution.
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- ` --loci_file LOCI_FILE ` Specify file with ids for loci to include.
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+ ` --loci_file LOCI_FILE ` Specify file with ids for loci to include.
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- ` --min_count MIN_COUNT ` Specify minimum coverage for a locus to be included for the sample.
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+ ` --min_count MIN_COUNT ` Specify minimum coverage for a locus to be included for the sample.
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- ` --min_found MIN_FOUND_IN ` Specify minimum number samples that a locus need to be present in to be included.
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+ ` --min_found MIN_FOUND_IN ` Specify minimum number samples that a locus need to be present in to be included.
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- ` --subsample SUBSAMPLE ` Specify coverage level at which to subsample.
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+ ` --subsample SUBSAMPLE ` Specify coverage level at which to subsample.
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- ` --keep_haplotypes ` If this is used, POGENOM will not split haplotypes into single-nucleotide variants, which is otherwise the default behaviour.
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- ` --vcf_version ` Specify VCF file format version. Can be set to 4.2 or 4.1 (default).
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+ ` --keep_haplotypes ` If this is used, POGENOM will not split haplotypes into single-nucleotide variants, which is otherwise the default behaviour.
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+ ` --vcf_version ` Specify VCF file format version. Can be set to 4.2 or 4.1 (default).
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- ` -h ` To print help message on screen.
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+ ` --help ` To print help message on screen.
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----
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