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FAQ

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FAQ


Below you can find some common error and problems you might face either during installations or during execution of softwares.

`Command line developer tools not found` (OSX)

If you don't yet have Mac OSX command line developer tools, please install it using:

xcode-select --install
`Error - umap-learn not found`

If your R does not find the correct python version, it will complain that umap-learn is not installed and ask you to install it. Here are some tips on how to find the correct python version that was installed in the conda environment.


Try selecting the correct conda env in R

In this example the conda environment is named scRNAseq2021.

library(reticulate)
reticulate::use_conda("scRNAseq2021")

Then check what python you have in R:

reticulate::py_config()
# should read at top:
python:         /Users/asbj/miniconda3/envs/scRNAseq2021/bin/python

If that still is not right, you may have an r-reticulate python installation as well and need to perform the steps below.


Restart R and select python version

OBS! Before doing anything else you need to select python version.

First, find out what path you have to your conda python (in TERMINAL):

which python
/Users/asbj/miniconda3/envs/scRNAseq2021/bin/python

Then in R (after restarting):

reticulate::use_python("/Users/asbj/miniconda3/envs/scRNAseq2021/bin/python", required=T)

Then check again with py_config if correct version of python is used:

reticulate::py_config()

If you have the correct version now, you should be able to run UMAP without issues.

`Unable to load stringi.so` (UNIX/Windows)

You can install stringi in R using:

install.packages('stringi')
`ERROR: Failed building wheel for gevent` / `MacOSX10.9.sdk missing` (MacOSX)

This is a problem with the MacOSX compiler, in which conda is unable to find it.

#Download MacOSX10.9.sdk from Github
curl -o MacOSX10.9.sdk.tar.gz "https://github-production-release-asset-2e65be.s3.amazonaws.com/13597203/f0123b00-34ab-11ea-84b1-27ccc324f983?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIAIWNJYAX4CSVEH53A%2F20210122%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Date=20210122T065821Z&X-Amz-Expires=300&X-Amz-Signature=e11864967b0c9a1e1ba1121dbadd35bb3129ae1cd87bdf07b1a9965c731ae129&X-Amz-SignedHeaders=host&actor_id=22674952&key_id=0&repo_id=13597203&response-content-disposition=attachment%3B%20filename%3DMacOSX10.9.sdk.tar.xz&response-content-type=application%2Foctet-stream"

#extract
sudo tar -xzf MacOSX10.9.sdk.tar.xz

#copy
sudo cp -r MacOSX10.9.sdk /opt/

#give executable permissions
sudo chmod -R a+rX /opt

#Link the path where conda looks to where the file is
ln -s /Applications/Xcode.app/Contents/Developer/Platforms/MacOSX.platform/Developer/SDKs/MacOSX.sdk /opt/MacOSX10.9.sdk

Back to main

As a last resource in case you are having installation issues, please try following these docker_instructions