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I would like to suggest some clarification regarding it. I am posting my ideas here, because I can't find a way to access salmon-tutorials repo.
the choice of -Y flag when created minimap references:
I think the -Y should be used during alignment, not during reference creation. https://lh3.github.io/minimap2/minimap2.html
the ErrorModel choice
The ONTAlignmentModel documentation is hard to find. But based on my understanding --ont --noErrorModel is not the same as --ont, in the 2021 guide, it is comparing --ont against --noErrorModel.
Other minior suggestions
the salmon -la flag explaination
The not immediately clear regarding this flag. I think it is a combination of the -l (library choice auto) and -a (alignment file). Perhaps it is worthy to add a brief description to the documentation.
-ax map-ont usage
credit Quantification of direct RNA seq reads (ONT) using salmon #786
The guide used -a -x map-ont flag in minimap2 which I think it is equivalent to -ax map-ont. It is perhaps a simple concept for others, but it take me a bit to follow. Perhaps it is worthy to add a brief description to the documentation.
The text was updated successfully, but these errors were encountered:
yinshiyi
changed the title
some questions on the long reads best pratices
best practices documentation on the ONT long reads
Dec 9, 2024
This link is referred many times in this forum as the best practice when analyzed ONT with Minimap2
https://combine-lab.github.io/salmon-tutorials/2021/ont-long-read-quantification/
eg. #924, #790
I would like to suggest some clarification regarding it. I am posting my ideas here, because I can't find a way to access
salmon-tutorials
repo.-Y
flag when created minimap references:I think the
-Y
should be used during alignment, not during reference creation.https://lh3.github.io/minimap2/minimap2.html
ErrorModel
choiceThe
ONTAlignmentModel
documentation is hard to find. But based on my understanding--ont --noErrorModel
is not the same as--ont
, in the 2021 guide, it is comparing--ont
against--noErrorModel
.Other minior suggestions
-la
flag explainationThe not immediately clear regarding this flag. I think it is a combination of the
-l
(library choice auto) and-a
(alignment file). Perhaps it is worthy to add a brief description to the documentation.-ax map-ont
usagecredit Quantification of direct RNA seq reads (ONT) using salmon #786
The guide used
-a -x map-ont
flag in minimap2 which I think it is equivalent to-ax map-ont
. It is perhaps a simple concept for others, but it take me a bit to follow. Perhaps it is worthy to add a brief description to the documentation.The text was updated successfully, but these errors were encountered: