From f16ce8e83d5a3ca68f9ddc3b36bb6e97b361ce3a Mon Sep 17 00:00:00 2001 From: BBQuercus Date: Wed, 29 Jun 2022 11:49:21 +0200 Subject: [PATCH] Convert FPKM to expression percentage & test suite --- README.md | 52 +- eFISHent/alignment.py | 203 +- eFISHent/cleanup.py | 17 +- eFISHent/config.py | 105 +- eFISHent/constants.py | 2 - tests/aad4_basic.sam | 48 +- tests/count_table1.tsv | 7229 +++++++++++++++++++++++++++++++++---- tests/count_table2.csv | 3026 ++++++++++++---- tests/renilla_basic.sam | 16 +- tests/test_alignment.py | 62 +- tests/test_integration.py | 10 +- 11 files changed, 9080 insertions(+), 1690 deletions(-) diff --git a/README.md b/README.md index e4771ac..5d23805 100644 --- a/README.md +++ b/README.md @@ -6,7 +6,7 @@ A tool to facilitate the creation of eFISHent RNA FISH oligonucleotide probes. eFISHent is a tool to facilitate the creation of eFISHent RNA FISH oligonucleotide probes. Some of the key features of eFISHent are: -* Single-line installation using conda +* One-line installation using conda (available through bioconda) * Automatic gene sequence download from NCBI when providing a gene and species name (or pass a FASTA file) * Filtering steps to remove low-quality probes: * Basics such as melting temperature, GC content, and length @@ -25,7 +25,7 @@ conda install -c bioconda efishent ## Usage -A detailed usage guide can be found on the [GitHub wiki]() but here is a quick example: +A detailed usage guide can be found on the [GitHub wiki](https://github.com/bbquercus/eFISHent/wiki) but here is a quick example: ```bash eFISHent --reference-genome --gene-name --organism-name @@ -58,45 +58,11 @@ Probe filtering workflow: * [x] Find a name and description for the tool * [x] Add basic documentation in CLI and in README.md * [x] Add thread limiting check with `os.cpu_count()` + * [x] Create bioconda recipe + * [ ] Add logo to repository * [ ] Add more detailed documentation as wiki page(s) - * [ ] Add mathematical description for model - * [ ] Create bioconda recipe - -* **Components** - * [x] Add spacing option (length +- nt in optimization step) - * [x] Remove gene of interest when using rna-seq data - * [x] Save alignment and kmer scores to csv output - * [x] Add GTF file reading & saving as parquet/csv step - * [x] Add EnsembleID support in entrez query - * [x] Verify bowtie parameters run on endo and exo targets - * [x] Select intron/exon based on blast from sequence - doesn't make sense given all different isoforms and variations - * [x] Allow selection of isoform in entrez query - not available / different transcript id's yield same sequence - -* **Interface** - * [x] Clean up output files - * [x] Clean up logging and error handling - * [x] Decide on way to handle temporary files (tempdir?) - * [x] Find way to handle rerunning same gene with different parameters (unique name hash?) - * [x] Find way to make CLI alter config (luigi.configuration.add_config_path) - * [x] Use final output file as indicator if pipeline finished running - * [x] Proper boolean support :monkey: - -* **Testing** - * [ ] Add unit tests for core components - * Sliding window probe generation - * Entrez query error handling - * Parsing alignment output - * Parsing blast output - * Selecting intron/exon - * RNA-seq data loading - * RNA-seq data gene of interest removal - * RNA-seq data sorting - * Secondary structure prediction - * Optimization (small mock cases) - mathematical model - * Optimization - greedy model - * [ ] Add integration tests for the following examples - * dm6/hr38 (download from entrez, endo, long, multiple isoforms) - * dm6/kayak (provided, select introns, endo) - * dm6/Renilla (provided, exo) - * hg38/RPL12 (provided, endo, repetitive, short, minus strand) - * hg38/VIM (provided, endo, plus strand) + * [ ] Add benchmarks for deltaG, FPKM + * [ ] Add links to genomes and RNAseq databases + * [ ] Add examples from multiple sources + * [ ] Add mathematical description for model (in wiki?) + * [ ] Add citation diff --git a/eFISHent/alignment.py b/eFISHent/alignment.py index a9f09b8..1b572ca 100644 --- a/eFISHent/alignment.py +++ b/eFISHent/alignment.py @@ -69,7 +69,7 @@ class PrepareAnnotationFile(luigi.Task): def output(self): return luigi.LocalTarget(GeneralConfig().reference_annotation + ".parq") - def prepare_gtf_file(self, fname_input: str, fname_output) -> None: + def prepare_gtf_file(self, fname_input: str, fname_output: str) -> None: """Save GTF file as parquet.""" warnings.filterwarnings("ignore") df = gtfparse.read_gtf(fname_input) @@ -86,24 +86,19 @@ class AlignProbeCandidates(luigi.Task): """Align all probe candidates to the reference genome and filter.""" logger = logging.getLogger("custom-logger") - is_blast_required = ( - ProbeConfig().encode_count_table and SequenceConfig().is_endogenous - ) - def _requires(self): - """Hidden / not directly accessed requirements.""" - tasks = [BuildBowtieIndex()] - if self.is_blast_required: - tasks.append(BuildBlastDatabase()) - return luigi.task.flatten( - [*tasks, super(AlignProbeCandidates, self)._requires()] + def __init__(self, *args, **kwargs): + super().__init__(*args, **kwargs) + self.is_blast_required = ( + ProbeConfig().encode_count_table and SequenceConfig().is_endogenous ) def requires(self): - tasks = {"probes": BasicFiltering()} + tasks = {"probes": BasicFiltering(), "bowtie": BuildBowtieIndex()} if self.is_blast_required: tasks["blastseq"] = PrepareSequence() tasks["gtf"] = PrepareAnnotationFile() + tasks["blast"] = BuildBlastDatabase() return tasks def output(self): @@ -116,28 +111,57 @@ def output(self): } if self.is_blast_required: tasks["table"] = luigi.LocalTarget( - os.path.join(util.get_output_dir(), f"{util.get_gene_name()}_fpkm.csv") + os.path.join( + util.get_output_dir(), f"{util.get_gene_name()}_counts.csv" + ) ) return tasks - def read_count_table(self, fname: str) -> pd.DataFrame: - """Read and verify a RNAseq FPKM count table.""" - df = pd.read_csv(fname, sep="\t") - required_columns = ["gene_id", *constants.COUNTS_COLUMNS[1:]] - if not all(col in df.columns for col in required_columns): + @property + def count_table(self) -> pd.DataFrame: + """Read and verify a RNAseq normalized count table.""" + if not os.path.splitext(self.fname_count)[1] == ".tsv": + raise ValueError( + "The count table must be provided as TSV file. " + f"Only found - {self.fname_count}" + ) + df = pd.read_csv(self.fname_count, sep="\t").reset_index().dropna() + if not len(df.columns) >= 2: raise ValueError( - f"The columns {required_columns} must be in the count table. " + "The count table must contain at least 2 columns." f"Only found - {df.columns}" ) + self.logger.debug(f"Mapping original the first two columns of - {df.columns}.") + self.logger.debug(f"Read count table with {len(df)} entries.") + self.logger.debug(f"Found dtypes - {df.dtypes}") + self.logger.debug(f"First column - {df.iloc[0]}") + + df = df.iloc[:, :2] + df.columns = ["gene_id", "count"] + try: + df = df.astype({"gene_id": str, "count": float}) + except ValueError: + raise ValueError( + "Could not convert count table columns to the right type. " + "Please ensure the first two colums are of type string and number/float/int, respectively. " + ) # Remove potential version numbers - df["clean_id"] = df["gene_id"].apply(lambda x: x.split(".")[0]) - self.logger.debug(f"Read count table with {len(df)} entries.") - return df[constants.COUNTS_COLUMNS] + df["gene_id"] = df["gene_id"].apply(lambda x: x.split(".")[0]) + return df - def read_gtf_file(self, fname: str): + @property + def gtf_table(self) -> pd.DataFrame: """Parse prepared parquet-formatted gtf file.""" - return pd.read_parquet(fname)[constants.GTF_COLUMNS] + return pd.read_parquet(self.fname_gtf)[constants.GTF_COLUMNS] + + @property + def norm_table(self) -> pd.DataFrame: + """Merge gtf and count table.""" + df = pd.merge( + self.count_table, self.gtf_table, how="left", on="gene_id" + ).dropna() + return df def align_probes(self, max_off_targets: int, is_endogenous: bool, threads: int): """Align probes to the reference genome. @@ -193,66 +217,58 @@ def filter_unique_probes(self, is_endogenous: bool) -> pd.DataFrame: return pd.DataFrame(data, columns=columns).dropna() return pd.DataFrame(columns=columns) - def prepare_fpkm_table( - self, fname_gtf: str, fname_count: str, **gene_of_interest - ) -> pd.DataFrame: - """Merge gtf and count table.""" - df_gtf = self.read_gtf_file(fname_gtf) - df_counts = self.read_count_table(fname_count) - df = pd.merge( - df_counts, df_gtf, how="left", left_on="clean_id", right_on="gene_id" - ).dropna() - return df - def exclude_gene_of_interest( self, df: pd.DataFrame, ensembl_id: str, fname_full_gene: str, threads: int ) -> pd.DataFrame: - """Filter FPKM table to exclude the gene of interest.""" + """Filter count table to exclude the gene of interest.""" # Filter using provided EnsemblID directly if ensembl_id: self.logger.debug(f"Filtering directly using EmsembleID {ensembl_id}") - return df[df["clean_id"] != ensembl_id] + return df[df["gene_id"] != ensembl_id] # Filter using blast - with tempfile.TemporaryDirectory() as tmp_dir: - args_blast = [ - "blastn", - "-db", - self.fname_genome, - "-query", - fname_full_gene, - "-task", - "megablast", - "-outfmt", - "6", - "-num_threads", - str(threads), - ] - self.logger.debug(f"Running blast with - {' '.join(args_blast)}") - process = subprocess.run(args_blast, capture_output=True, text=True) - data = [row.split("\t") for row in process.stdout.split("\n") if row] - df_blast = pd.DataFrame(data, columns=constants.BLAST_COLUMNS).astype( - {name: float for name in ["pident", "sstart", "send", "evalue"]} - ) + args_blast = [ + "blastn", + "-db", + self.fname_genome, + "-query", + fname_full_gene, + "-task", + "megablast", + "-outfmt", + "6", + "-num_threads", + str(threads), + ] + self.logger.debug(f"Running blast with - {' '.join(args_blast)}") + process = subprocess.run(args_blast, capture_output=True, text=True) + data = [row.split("\t") for row in process.stdout.split("\n") if row] + df_blast = pd.DataFrame(data, columns=constants.BLAST_COLUMNS).astype( + {name: float for name in ["pident", "sstart", "send", "evalue"]} + ) # Arbirary identity values to ensure similar targets aren't falsely selected df_blast = df_blast[ (df_blast["pident"] >= 98) - & (df_blast["evalue"] <= 1e-8) + & (df_blast["evalue"] <= 1e-5) & (df_blast["sseqid"] == df_blast.loc[0, "sseqid"]) ] # Pad blast start/end results in case not everything was found/isoforms/etc. # Set to an arbitrary maximum value of 100 to not include other genes + # Account for forward and reverse direction for buffer in range(0, 110, 10): gene_ids = [] for _, row in df_blast.iterrows(): gene_ids.extend( df[ - (df["seqname"] == row["sseqid"]) - & (df["start"] >= row["sstart"] - buffer) - & (df["end"] <= row["send"] + buffer) - ]["clean_id"].values + ( + (df["seqname"] == row["sseqid"]) + | (df["seqname"] == f"chr{row['sseqid']}") + ) + & ((df["start"] >= row["sstart"]) & (df["end"] <= row["send"])) + | ((df["start"] >= row["send"]) & (df["end"] <= row["sstart"])) + ]["gene_id"].values ) if not gene_ids: self.logger.debug(f"No genes found using a buffer of {buffer}") @@ -262,22 +278,24 @@ def exclude_gene_of_interest( self.logger.debug( f"Filtering out most frequent gene {most_frequent_gene_id}" ) - df = df[df["clean_id"] != most_frequent_gene_id].reset_index(drop=True) + df = df[df["gene_id"] != most_frequent_gene_id].reset_index(drop=True) break return df - def get_maximum_fpkm( - self, df_fpkm: pd.DataFrame, df_sam: pd.DataFrame + def join_alignment_with_annotation( + self, df_sam: pd.DataFrame, df_norm: pd.DataFrame ) -> pd.DataFrame: - """Find the highest FPKM values across detected off-targets.""" - # Match FPKM to the probe candidates' off-target genomic locations - # Loop over chromosome/rname to keep loci separated + """Merge probe alignment with normalized count table based on start/end positions.""" dfs = [] + for sequence, df_sequence in df_sam.groupby("rname"): - df_fpkm_sequence = df_fpkm[df_fpkm["seqname"] == sequence] - gene_start = df_fpkm_sequence["start"].astype(int).values - gene_end = df_fpkm_sequence["end"].astype(int).values + df_count_sequence = df_norm[ + (df_norm["seqname"] == sequence) + | (df_norm["seqname"] == sequence.lstrip("chr")) + ] + gene_start = df_count_sequence["start"].astype(int).values + gene_end = df_count_sequence["end"].astype(int).values align_start = df_sequence["pos"].astype(int).values align_end = align_start + df_sequence["seq"].apply(len).values @@ -293,11 +311,11 @@ def get_maximum_fpkm( ) ) - # Join columns to merge alignment information with FPKM values + # Join columns to merge alignment information with count values df_sequence = pd.concat( [ df_sequence.reset_index(drop=True).iloc[i].reset_index(drop=True), - df_fpkm_sequence.reset_index(drop=True) + df_count_sequence.reset_index(drop=True) .iloc[j] .reset_index(drop=True), ], @@ -305,37 +323,44 @@ def get_maximum_fpkm( ) dfs.append(df_sequence) dfs = pd.concat(dfs, ignore_index=True) + return dfs - # Get the highest FPKM per alignment/candidate - df_max_fpkm = dfs.groupby("qname", as_index=False)["FPKM"].max() - return df_max_fpkm + def get_most_expressed_genes(self, df: pd.DataFrame, percentage: float) -> list: + """Find the most highly expressed genes based on normalized count.""" + quantile = df["count"].quantile(percentage / 100) + most_expressed = df[df["count"] > quantile].reset_index(drop=True) + return most_expressed["gene_id"].values.tolist() # TODO allow filtering if exogenous? - def filter_using_fpkm(self, df_sam: pd.DataFrame) -> pd.DataFrame: + def filter_using_count(self, df_sam: pd.DataFrame) -> pd.DataFrame: """Filter candidates binding off targets with too high FPKMs.""" - df_fpkm = self.prepare_fpkm_table( - fname_gtf=self.input()["gtf"].path, - fname_count=ProbeConfig().encode_count_table, - ) - df_fpkm = self.exclude_gene_of_interest( - df_fpkm, + df = self.exclude_gene_of_interest( + self.norm_table, ensembl_id=SequenceConfig().ensembl_id, fname_full_gene=self.input()["blastseq"].path, threads=GeneralConfig().threads, ) - df_max_fpkm = self.get_maximum_fpkm(df_sam, df_fpkm) - df_max_fpkm.to_csv(self.output()["table"].path, index=False) - df_sam = df_max_fpkm[df_max_fpkm["FPKM"] <= ProbeConfig().max_off_target_fpkm] + df = self.join_alignment_with_annotation(df_sam=df_sam, df_norm=df) + most_expressed = self.get_most_expressed_genes( + df=self.norm_table, percentage=ProbeConfig().max_expression_percentage + ) + df.to_csv(self.output()["table"].path, index=False) + df_sam = df[~df["gene_id"].isin(most_expressed)].reset_index(drop=True) return df_sam def run(self): + self.logger.warning(f"is required - {self.is_blast_required}") + self.logger.warning(ProbeConfig()) + # Naming self.fname_fasta = self.input()["probes"].path self.fname_sam = os.path.splitext(self.fname_fasta)[0] + ".sam" self.fname_genome = util.get_genome_name() self.fname_gene = util.get_gene_name() + self.fname_gtf = self.input()["gtf"].path + self.fname_count = ProbeConfig().encode_count_table - # Alignment + # Alignment and filtering self.align_probes( max_off_targets=ProbeConfig().max_off_targets, is_endogenous=SequenceConfig().is_endogenous, @@ -343,9 +368,9 @@ def run(self): ) df_sam = self.filter_unique_probes(is_endogenous=SequenceConfig().is_endogenous) if self.is_blast_required: - df_sam = self.filter_using_fpkm(df_sam) + df_sam = self.filter_using_count(df_sam) - # Filter candidates found in samfile + # Save output sequences = list(Bio.SeqIO.parse(self.fname_fasta, "fasta")) candidates = [seq for seq in sequences if seq.id in df_sam["qname"].values] util.log_and_check_candidates( diff --git a/eFISHent/cleanup.py b/eFISHent/cleanup.py index f4e7260..94db3c0 100644 --- a/eFISHent/cleanup.py +++ b/eFISHent/cleanup.py @@ -32,9 +32,12 @@ class CleanUpOutput(luigi.Task): """Clean up the output files and remove the intermediaries that are not needed.""" logger = logging.getLogger("custom-logger") - is_blast_required = ( - ProbeConfig().encode_count_table and SequenceConfig().is_endogenous - ) + + def __init__(self, *args, **kwargs): + super().__init__(*args, **kwargs) + self.is_blast_required = ( + ProbeConfig().encode_count_table and SequenceConfig().is_endogenous + ) def requires(self): tasks = { @@ -81,10 +84,10 @@ def prettify_table( df["deltaG"] = [get_free_energy(seq) for seq in sequences] df["kmers"] = [get_max_kmer_count(seq, jellyfish_path) for seq in sequences] if alignment_path is not None: - df_fpkm = pd.read_csv(alignment_path) - df["FPKM"] = pd.merge( - df, df_fpkm, how="left", left_on="name", right_on="qname" - )["FPKM"].fillna(0) + df_counts = pd.read_csv(alignment_path) + df["counts"] = pd.merge( + df, df_counts, how="left", left_on="name", right_on="qname" + )["counts"].fillna(0) # Create new/clean names df["name"] = [f"{basename}-{idx + 1}" for idx in df.index] diff --git a/eFISHent/config.py b/eFISHent/config.py index 63f8d13..4d7fdbc 100644 --- a/eFISHent/config.py +++ b/eFISHent/config.py @@ -1,28 +1,4 @@ -""" -Processes: - - index creation - - probe generation - -General options: - - reference genome -> pass file, will check if index exists in same folder - - reference genome annotation - - ENCODE RNAseq count table for off-target weighting - - number of threads - - method of optimization - - optimization time limit - -Probe specific options: - - (sequence file) or (gene name & organism) - - strand on genome - - intronic/exonic/both - - endogenous or exogenous - - min and max length - - min and max melting temperature - - min and max GC content - - formamide concentration - - salt concentration - - maximum deltaG -""" +"""Luigi configurations passed as command line arguments.""" import luigi @@ -39,8 +15,10 @@ class GeneralConfig(luigi.Config): ) threads = luigi.IntParameter(description="Number of threads to launch.", default=2) output_dir = luigi.Parameter( - description="Path to output directory. " - "If not specified, will use the current directory.", + description=( + "Path to output directory. " + "If not specified, will use the current directory." + ), default="", ) @@ -53,31 +31,39 @@ class RunConfig(luigi.Config): description="Save intermediate files?", default=False ) optimization_method = luigi.Parameter( - description="Optimization method to use. " - "Greedy will procedurally select the next longest probe. " - "Optimal will optimize probes for maximum gene coverage - " - "slow if many overlaps are present (typically with non-stringent parameter settings). " - "[options: optimal, greedy].", + description=( + "Optimization method to use. " + "Greedy will procedurally select the next longest probe. " + "Optimal will optimize probes for maximum gene coverage - " + "slow if many overlaps are present (typically with non-stringent parameter settings). " + "[options: optimal, greedy]." + ), default="greedy", ) optimization_time_limit = luigi.IntParameter( - description="Time limit for optimization in seconds. " - "Only used if optimization method is set to 'optimal'.", + description=( + "Time limit for optimization in seconds. " + "Only used if optimization method is set to 'optimal'." + ), default=60, ) class SequenceConfig(luigi.Config): sequence_file = luigi.Parameter( - description="Path to the gene's sequence file. " - "Use this if the sequence can't be easily downloaded or if only certain regions should be targeted.", + description=( + "Path to the gene's sequence file. " + "Use this if the sequence can't be easily downloaded or if only certain regions should be targeted." + ), default="", ) ensembl_id = luigi.Parameter( - description="Ensembl ID of the gene of interest. " - "Can be used instead of gene and organism name to download the gene of interest. " - "Used to filter out the gene of interest from FPKM based filtering - " - "will do an automated blast-based filtering if not passed.", + description=( + "Ensembl ID of the gene of interest. " + "Can be used instead of gene and organism name to download the gene of interest. " + "Used to filter out the gene of interest from FPKM based filtering - " + "will do an automated blast-based filtering if not passed." + ), default="", ) gene_name = luigi.Parameter(description="Gene name.", default="") @@ -103,8 +89,10 @@ class ProbeConfig(luigi.Config): description="Minimum distance in nucleotides between probes.", default=2 ) min_tm = luigi.FloatParameter( - description="Minimum melting temperature in degrees C. " - "Formamide and Na concentration will affect melting temperature!", + description=( + "Minimum melting temperature in degrees C. " + "Formamide and Na concentration will affect melting temperature!" + ), default=40.0, ) max_tm = luigi.FloatParameter( @@ -125,23 +113,30 @@ class ProbeConfig(luigi.Config): description="Na concentration in mM.", default=390.0 ) max_off_targets = luigi.IntParameter( - description="Maximum number of off-targets binding anywhere BUT " - "the gene of interest in the genome.", + description=( + "Maximum number of off-targets binding anywhere BUT " + "the gene of interest in the genome." + ), default=0, ) encode_count_table = luigi.Parameter( - description="Path to the ENCODE RNAseq count table. " - "Must contain the columns 'gene_id' and 'FPKM'. " - "'gene_id' must use ensembl ID's!", + description=( + "Path to the ENCODE RNAseq count table in TSV format. " + "The first two columns have to be `gene_id` and `normalized_value` respectively. " + "Specific column names are not required. " + "`gene_id` have to be ensemble IDs of the respective genes. " + "`normalized_value` have to be normalized count values (RPKM, FPKM, TPM, DeSeq2-norm, etc). " + ), default="", ) - max_off_target_fpkm = luigi.FloatParameter( + max_expression_percentage = luigi.FloatParameter( description=( - "Maximum off-target FPKM (Fragments Per Kilobase of transcript per Million mapped reads) " - "based on ENCODE RNAseq count table. " - "Only used if ENCODE RNAseq count table is provided." + "Remove any off-target genes expressed more highly than " + "`max-expression-percentage` percentage of all genes. " + "Genes to remove are based on ENCODE RNAseq count table provided in `encode-count-table`. " + "Only used if `encode-count-table` and `reference-annotation` are provided." ), - default=10.0, + default=50.0, ) kmer_length = luigi.IntParameter( description="Length of k-mer used to filter probe sequences.", default=15 @@ -150,7 +145,9 @@ class ProbeConfig(luigi.Config): description="Highest count of sub-k-mers found in reference genome.", default=5 ) max_deltag = luigi.FloatParameter( - description="Maximum predicted deltaG in kcal/mol. " - "Note: deltaGs range from 0 (no secondary structures) to increasingly negative values!", + description=( + "Maximum predicted deltaG in kcal/mol. " + "Note: deltaGs range from 0 (no secondary structures) to increasingly negative values!" + ), default=-10.0, ) diff --git a/eFISHent/constants.py b/eFISHent/constants.py index a6ea144..4b21c28 100644 --- a/eFISHent/constants.py +++ b/eFISHent/constants.py @@ -35,8 +35,6 @@ "bitscore", ] -COUNTS_COLUMNS = ["clean_id", "length", "FPKM"] - GTF_COLUMNS = [ "gene_id", "seqname", diff --git a/tests/aad4_basic.sam b/tests/aad4_basic.sam index ee7120e..b87c95f 100644 --- a/tests/aad4_basic.sam +++ b/tests/aad4_basic.sam @@ -17,48 +17,48 @@ @SQ SN:chrXVI LN:948066 @SQ SN:chrM LN:85779 @PG ID:Bowtie VN:1.0.0 CL:"bowtie ./tests/sacCer3 ./tests/aad4_basic.fastq --threads 2 --sam -S ./tests/aad4_basic.sam" -candidate-1-0 16 chrXIV 17090 255 21M * 0 0 TTCCTTATTCATAGAGCCCAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-2-1 16 chrIV 18545 255 21M * 0 0 GTTCCTTATTCATAGAGCCCA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-3-2 16 chrXIV 17088 255 21M * 0 0 TGTTCCTTATTCATAGAGCCC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-1-0 16 chrXIV 17090 255 21M * 0 0 TTCCTTATTCATAGAGCCCAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-4-3 16 chrXIV 17087 255 21M * 0 0 CTGTTCCTTATTCATAGAGCC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-3-2 16 chrXIV 17088 255 21M * 0 0 TGTTCCTTATTCATAGAGCCC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-5-4 16 chrXIV 17086 255 21M * 0 0 CCTGTTCCTTATTCATAGAGC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-6-5 16 chrXIV 17085 255 21M * 0 0 GCCTGTTCCTTATTCATAGAG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-7-6 16 chrIV 18540 255 21M * 0 0 AGCCTGTTCCTTATTCATAGA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-8-7 16 chrIV 18539 255 21M * 0 0 AAGCCTGTTCCTTATTCATAG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-9-8 16 chrIV 18538 255 21M * 0 0 AAAGCCTGTTCCTTATTCATA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-10-9 16 chrIV 18537 255 21M * 0 0 AAAAGCCTGTTCCTTATTCAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-11-10 16 chrIV 18536 255 21M * 0 0 CAAAAGCCTGTTCCTTATTCA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-10-9 16 chrIV 18537 255 21M * 0 0 AAAAGCCTGTTCCTTATTCAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-12-11 16 chrIV 18535 255 21M * 0 0 TCAAAAGCCTGTTCCTTATTC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-13-12 16 chrIV 18534 255 21M * 0 0 TTCAAAAGCCTGTTCCTTATT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-14-13 16 chrIV 18533 255 21M * 0 0 GTTCAAAAGCCTGTTCCTTAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-13-12 16 chrIV 18534 255 21M * 0 0 TTCAAAAGCCTGTTCCTTATT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-15-14 16 chrIV 18532 255 21M * 0 0 AGTTCAAAAGCCTGTTCCTTA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-19-18 16 chrIV 18528 255 21M * 0 0 AAGAAGTTCAAAAGCCTGTTC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-20-19 16 chrIV 18527 255 21M * 0 0 CAAGAAGTTCAAAAGCCTGTT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-19-18 16 chrIV 18528 255 21M * 0 0 AAGAAGTTCAAAAGCCTGTTC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-22-21 16 chrIV 18525 255 21M * 0 0 ATCAAGAAGTTCAAAAGCCTG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-23-22 16 chrIV 18524 255 21M * 0 0 CATCAAGAAGTTCAAAAGCCT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-25-24 16 chrIV 18522 255 21M * 0 0 AGCATCAAGAAGTTCAAAAGC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-26-25 16 chrIV 18521 255 21M * 0 0 AAGCATCAAGAAGTTCAAAAG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-27-26 16 chrIV 18520 255 21M * 0 0 AAAGCATCAAGAAGTTCAAAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-26-25 16 chrIV 18521 255 21M * 0 0 AAGCATCAAGAAGTTCAAAAG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-28-27 16 chrIV 18519 255 21M * 0 0 AAAAGCATCAAGAAGTTCAAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-29-28 16 chrIV 18518 255 21M * 0 0 AAAAAGCATCAAGAAGTTCAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-30-29 16 chrXIV 17061 255 21M * 0 0 TAAAAAGCATCAAGAAGTTCA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-29-28 16 chrIV 18518 255 21M * 0 0 AAAAAGCATCAAGAAGTTCAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-31-30 16 chrIV 18516 255 21M * 0 0 ATAAAAAGCATCAAGAAGTTC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-32-31 16 chrIV 18515 255 21M * 0 0 CATAAAAAGCATCAAGAAGTT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-33-32 16 chrXIV 17058 255 21M * 0 0 TCATAAAAAGCATCAAGAAGT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-32-31 16 chrIV 18515 255 21M * 0 0 CATAAAAAGCATCAAGAAGTT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-34-33 16 chrIV 18513 255 21M * 0 0 TTCATAAAAAGCATCAAGAAG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-35-34 16 chrIV 18512 255 21M * 0 0 CTTCATAAAAAGCATCAAGAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-36-35 16 chrXIV 17055 255 21M * 0 0 GCTTCATAAAAAGCATCAAGA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-35-34 16 chrIV 18512 255 21M * 0 0 CTTCATAAAAAGCATCAAGAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-37-36 16 chrIV 18510 255 21M * 0 0 TGCTTCATAAAAAGCATCAAG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-38-37 16 chrIV 18509 255 21M * 0 0 CTGCTTCATAAAAAGCATCAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-39-38 16 chrIV 18508 255 21M * 0 0 CCTGCTTCATAAAAAGCATCA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-40-39 16 chrIV 18507 255 21M * 0 0 TCCTGCTTCATAAAAAGCATC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-41-40 16 chrIV 18506 255 21M * 0 0 CTCCTGCTTCATAAAAAGCAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-40-39 16 chrIV 18507 255 21M * 0 0 TCCTGCTTCATAAAAAGCATC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-44-43 16 chrIV 18503 255 21M * 0 0 TACCTCCTGCTTCATAAAAAG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-45-44 16 chrIV 18502 255 21M * 0 0 TTACCTCCTGCTTCATAAAAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-46-45 16 chrIV 18501 255 21M * 0 0 ATTACCTCCTGCTTCATAAAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-45-44 16 chrIV 18502 255 21M * 0 0 TTACCTCCTGCTTCATAAAAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-47-46 16 chrIV 18500 255 21M * 0 0 AATTACCTCCTGCTTCATAAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-48-47 16 chrIV 18499 255 21M * 0 0 CAATTACCTCCTGCTTCATAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-56-55 16 chrIV 18491 255 21M * 0 0 TATCAATGCAATTACCTCCTG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-48-47 16 chrIV 18499 255 21M * 0 0 CAATTACCTCCTGCTTCATAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-57-56 16 chrIV 18490 255 21M * 0 0 GTATCAATGCAATTACCTCCT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-58-57 16 chrIV 18489 255 21M * 0 0 AGTATCAATGCAATTACCTCC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-59-58 16 chrIV 18488 255 21M * 0 0 CAGTATCAATGCAATTACCTC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 @@ -77,9 +77,9 @@ candidate-72-71 16 chrXIV 17019 255 21M * 0 0 TGGTAACTGTTTGCAGTATCA IIIIIIIIIIII candidate-73-72 16 chrIV 18474 255 21M * 0 0 TTGGTAACTGTTTGCAGTATC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-74-73 16 chrIV 18473 255 21M * 0 0 TTTGGTAACTGTTTGCAGTAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-75-74 16 chrXIV 17016 255 21M * 0 0 TTTTGGTAACTGTTTGCAGTA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-76-75 16 chrXIV 17015 255 21M * 0 0 ATTTTGGTAACTGTTTGCAGT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-77-76 16 chrIV 18470 255 21M * 0 0 CATTTTGGTAACTGTTTGCAG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-78-77 16 chrXIV 17013 255 21M * 0 0 TCATTTTGGTAACTGTTTGCA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-76-75 16 chrXIV 17015 255 21M * 0 0 ATTTTGGTAACTGTTTGCAGT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-79-78 16 chrIV 18468 255 21M * 0 0 TTCATTTTGGTAACTGTTTGC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-80-79 16 chrXIV 17011 255 21M * 0 0 CTTCATTTTGGTAACTGTTTG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-81-80 16 chrXIV 17010 255 21M * 0 0 TCTTCATTTTGGTAACTGTTT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 @@ -162,34 +162,34 @@ candidate-178-177 16 chrXIV 16913 255 21M * 0 0 TTCATACTTCTTATAATCTCC IIIIIIIIII candidate-179-178 16 chrXIV 16912 255 21M * 0 0 CTTCATACTTCTTATAATCTC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-180-179 16 chrXIV 16911 255 21M * 0 0 ACTTCATACTTCTTATAATCT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-181-180 16 chrIV 18366 255 21M * 0 0 TACTTCATACTTCTTATAATC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-182-181 16 chrIV 18365 255 21M * 0 0 CTACTTCATACTTCTTATAAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-183-182 16 chrXIV 16908 255 21M * 0 0 CCTACTTCATACTTCTTATAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-182-181 16 chrIV 18365 255 21M * 0 0 CTACTTCATACTTCTTATAAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-184-183 16 chrXIV 16907 255 21M * 0 0 ACCTACTTCATACTTCTTATA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-185-184 16 chrIV 18362 255 21M * 0 0 CACCTACTTCATACTTCTTAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-186-185 16 chrXIV 16905 255 21M * 0 0 CCACCTACTTCATACTTCTTA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-187-186 16 chrIV 18360 255 21M * 0 0 GCCACCTACTTCATACTTCTT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-207-206 16 chrIV 18340 255 21M * 0 0 CAATAGTTGGCACTTTTACCG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-208-207 16 chrIV 18339 255 21M * 0 0 ACAATAGTTGGCACTTTTACC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-209-208 16 chrIV 18338 255 21M * 0 0 CACAATAGTTGGCACTTTTAC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-208-207 16 chrIV 18339 255 21M * 0 0 ACAATAGTTGGCACTTTTACC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-210-209 16 chrIV 18337 255 21M * 0 0 CCACAATAGTTGGCACTTTTA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-212-211 16 chrIV 18335 255 21M * 0 0 TACCACAATAGTTGGCACTTT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-213-212 16 chrIV 18334 255 21M * 0 0 TTACCACAATAGTTGGCACTT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-212-211 16 chrIV 18335 255 21M * 0 0 TACCACAATAGTTGGCACTTT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-214-213 16 chrIV 18333 255 21M * 0 0 ATTACCACAATAGTTGGCACT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-215-214 16 chrIV 18332 255 21M * 0 0 GATTACCACAATAGTTGGCAC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-216-215 16 chrIV 18331 255 21M * 0 0 TGATTACCACAATAGTTGGCA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-215-214 16 chrIV 18332 255 21M * 0 0 GATTACCACAATAGTTGGCAC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-217-216 16 chrIV 18330 255 21M * 0 0 GTGATTACCACAATAGTTGGC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-218-217 16 chrIV 18329 255 21M * 0 0 TGTGATTACCACAATAGTTGG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-219-218 16 chrIV 18328 255 21M * 0 0 TTGTGATTACCACAATAGTTG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-218-217 16 chrIV 18329 255 21M * 0 0 TGTGATTACCACAATAGTTGG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-220-219 16 chrIV 18327 255 21M * 0 0 CTTGTGATTACCACAATAGTT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-221-220 16 chrIV 18326 255 21M * 0 0 GCTTGTGATTACCACAATAGT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-222-221 16 chrIV 18325 255 21M * 0 0 TGCTTGTGATTACCACAATAG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-223-222 16 chrIV 18324 255 21M * 0 0 ATGCTTGTGATTACCACAATA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-224-223 16 chrIV 18323 255 21M * 0 0 TATGCTTGTGATTACCACAAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-223-222 16 chrIV 18324 255 21M * 0 0 ATGCTTGTGATTACCACAATA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-225-224 16 chrIV 18322 255 21M * 0 0 CTATGCTTGTGATTACCACAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-226-225 16 chrIV 18321 255 21M * 0 0 ACTATGCTTGTGATTACCACA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-227-226 16 chrIV 18320 255 21M * 0 0 AACTATGCTTGTGATTACCAC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-228-227 16 chrIV 18319 255 21M * 0 0 AAACTATGCTTGTGATTACCA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-229-228 16 chrIV 18318 255 21M * 0 0 TAAACTATGCTTGTGATTACC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-228-227 16 chrIV 18319 255 21M * 0 0 AAACTATGCTTGTGATTACCA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-230-229 16 chrIV 18317 255 21M * 0 0 GTAAACTATGCTTGTGATTAC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-231-230 16 chrIV 18316 255 21M * 0 0 TGTAAACTATGCTTGTGATTA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-232-231 16 chrIV 18315 255 21M * 0 0 ATGTAAACTATGCTTGTGATT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 @@ -205,8 +205,8 @@ candidate-241-240 16 chrIV 18306 255 21M * 0 0 CACACTCACATGTAAACTATG IIIIIIIIIII candidate-242-241 16 chrIV 18305 255 21M * 0 0 TCACACTCACATGTAAACTAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-243-242 0 chrIII 314261 255 21M * 0 0 TAGTTTACATGTGAGTGTGAG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-244-243 0 chrIII 314262 255 21M * 0 0 AGTTTACATGTGAGTGTGAGG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-276-275 0 chrIII 314294 255 21M * 0 0 CAAATTGCAAACTGATTGGAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-277-276 0 chrIII 314295 255 21M * 0 0 AAATTGCAAACTGATTGGATT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-276-275 0 chrIII 314294 255 21M * 0 0 CAAATTGCAAACTGATTGGAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-278-277 0 chrIII 314296 255 21M * 0 0 AATTGCAAACTGATTGGATTG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-279-278 0 chrIII 314297 255 21M * 0 0 ATTGCAAACTGATTGGATTGA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-280-279 0 chrX 727558 255 21M * 0 0 TTGCAAACTGATTGGATTGAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 @@ -239,8 +239,8 @@ candidate-313-312 0 chrIII 314331 255 21M * 0 0 CACTGGTGGGATTATATGAGT IIIIIIIIII candidate-314-313 0 chrIII 314332 255 21M * 0 0 ACTGGTGGGATTATATGAGTT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-315-314 0 chrIII 314333 255 21M * 0 0 CTGGTGGGATTATATGAGTTC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-316-315 0 chrIII 314334 255 21M * 0 0 TGGTGGGATTATATGAGTTCA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-317-316 0 chrIII 314335 255 21M * 0 0 GGTGGGATTATATGAGTTCAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-318-317 0 chrIII 314336 255 21M * 0 0 GTGGGATTATATGAGTTCAAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-317-316 0 chrIII 314335 255 21M * 0 0 GGTGGGATTATATGAGTTCAA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-319-318 0 chrIII 314337 255 21M * 0 0 TGGGATTATATGAGTTCAATC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-320-319 0 chrIII 314338 255 21M * 0 0 GGGATTATATGAGTTCAATCG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-321-320 0 chrIII 314339 255 21M * 0 0 GGATTATATGAGTTCAATCGA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 @@ -262,10 +262,10 @@ candidate-336-335 16 chrXIV 16755 255 21M * 0 0 CTATCCATAACTTCTTCGATT IIIIIIIIII candidate-337-336 16 chrIV 18210 255 21M * 0 0 ACTATCCATAACTTCTTCGAT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-338-337 16 chrXIV 16753 255 21M * 0 0 AACTATCCATAACTTCTTCGA IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-339-338 16 chrIV 18208 255 21M * 0 0 AAACTATCCATAACTTCTTCG IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-340-339 16 chrXIV 16751 255 21M * 0 0 CAAACTATCCATAACTTCTTC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-341-340 16 chrIV 18206 255 21M * 0 0 GCAAACTATCCATAACTTCTT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 -candidate-342-341 16 chrXIV 16749 255 21M * 0 0 TGCAAACTATCCATAACTTCT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-340-339 16 chrXIV 16751 255 21M * 0 0 CAAACTATCCATAACTTCTTC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-343-342 16 chrIV 18204 255 21M * 0 0 ATGCAAACTATCCATAACTTC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 +candidate-342-341 16 chrXIV 16749 255 21M * 0 0 TGCAAACTATCCATAACTTCT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-344-343 16 chrIV 18203 255 21M * 0 0 TATGCAAACTATCCATAACTT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-345-344 16 chrXIV 16746 255 21M * 0 0 ATATGCAAACTATCCATAACT IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 candidate-346-345 16 chrIV 18201 255 21M * 0 0 AATATGCAAACTATCCATAAC IIIIIIIIIIIIIIIIIIIII XA:i:0 MD:Z:21 NM:i:0 diff --git a/tests/count_table1.tsv b/tests/count_table1.tsv index d8a59c6..a1239e6 100644 --- a/tests/count_table1.tsv +++ b/tests/count_table1.tsv @@ -1,716 +1,6513 @@ -gene_id transcript_id(s) length effective_length expected_count TPM FPKM posterior_mean_count posterior_standard_deviation_of_count pme_TPM pme_FPKM TPM_ci_lower_bound TPM_ci_upper_bound TPM_coefficient_of_quartile_variation FPKM_ci_lower_bound FPKM_ci_upper_bound FPKM_coefficient_of_quartile_variation -36097 36097 72.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36098 36098 72.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36099 36099 72.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36100 36100 72.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36101 36101 72.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36102 36102 72.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36103 36103 74.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36104 36104 72.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36105 36105 73.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36231 36231 83.00 8.00 0.00 0.00 0.00 0.00 0.00 11.10 10.99 0.000247462 33.152 0.658321 0.000245163 32.8232 0.658248 -36232 36232 73.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36308 36308 74.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36332 36332 72.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36393 36393 71.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36543 36543 73.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -36544 36544 73.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -4123 4123 75.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -4761 4761 75.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -5499 5499 72.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -5500 5500 73.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -6953 6953 70.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -6954 6954 72.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -8414 8414 93.00 18.00 0.00 0.00 0.00 0.00 0.00 4.93 4.89 0.000100762 14.8269 0.656917 9.9826e-05 14.6888 0.656844 -8789 8789 82.00 7.00 0.00 0.00 0.00 0.00 0.00 12.69 12.56 6.41084e-05 37.7352 0.657058 6.34766e-05 37.3952 0.657114 -ENSG00000000003.14 ENST00000373020.8,ENST00000494424.1,ENST00000496771.5,ENST00000612152.4,ENST00000614008.4 1856.81 1781.81 587.00 31.28 29.51 587.00 0.00 29.49 29.21 26.0347 33.0527 0.0407789 25.744 32.7007 0.0407828 -ENSG00000000005.5 ENST00000373031.4,ENST00000485971.1 542.00 467.00 1.00 0.20 0.19 1.00 0.00 0.42 0.41 0.0316965 0.944971 0.422379 0.0308437 0.935056 0.422242 -ENSG00000000419.12 ENST00000371582.8,ENST00000371584.8,ENST00000371588.9,ENST00000413082.1,ENST00000466152.5,ENST00000494752.1 1002.56 927.56 718.00 73.51 69.33 718.00 0.00 69.06 68.38 63.1761 75.2439 0.0304069 62.5238 74.4829 0.030424 -ENSG00000000457.13 ENST00000367770.5,ENST00000367771.10,ENST00000367772.8,ENST00000423670.1,ENST00000470238.1 2535.67 2460.67 51.00 1.97 1.86 51.00 0.00 2.01 1.99 1.39228 2.63172 0.106749 1.38017 2.60839 0.106716 -ENSG00000000460.16 ENST00000286031.10,ENST00000359326.8,ENST00000413811.3,ENST00000459772.5,ENST00000466580.6,ENST00000472795.5,ENST00000481744.5,ENST00000496973.5,ENST00000498289.5 2286.96 2211.96 219.00 9.40 8.87 219.00 0.00 9.48 9.39 7.50582 11.5019 0.0726677 7.43322 11.3889 0.0726199 -ENSG00000000938.12 ENST00000374003.7,ENST00000374004.5,ENST00000374005.7,ENST00000399173.5,ENST00000457296.5,ENST00000468038.1,ENST00000475472.5 1104.00 1029.00 6.00 0.55 0.52 6.00 0.00 0.95 0.94 0.41389 1.54943 0.214594 0.40909 1.53381 0.214692 -ENSG00000000971.15 ENST00000359637.2,ENST00000367429.8,ENST00000466229.5,ENST00000470918.1,ENST00000496761.1,ENST00000630130.2 2605.70 2530.70 3637.02 136.48 128.72 3637.43 14.61 128.25 127.00 123.171 133.373 0.0138384 122.056 132.136 0.0138101 -ENSG00000001036.13 ENST00000002165.10,ENST00000367585.1,ENST00000451668.1 2356.00 2281.00 721.00 30.02 28.31 721.00 0.00 28.47 28.19 26.3495 30.5719 0.0257607 26.1121 30.2893 0.0257323 -ENSG00000001084.12 ENST00000229416.10,ENST00000504353.1,ENST00000504525.1,ENST00000505197.1,ENST00000505294.5,ENST00000509541.5,ENST00000510837.5,ENST00000513939.6,ENST00000514004.5,ENST00000514373.3,ENST00000514933.2,ENST00000515580.1,ENST00000616923.5,ENST00000643939.1,ENST00000650454.1 930.61 855.61 1336.00 148.28 139.85 1336.00 0.00 140.21 138.85 132.262 148.701 0.0202183 130.867 147.174 0.0201424 -ENSG00000001167.14 ENST00000341376.10,ENST00000353205.5 2802.13 2727.13 481.00 16.75 15.80 481.00 0.00 15.72 15.56 14.0226 17.47 0.0381047 13.88 17.2936 0.0381112 -ENSG00000001460.17 ENST00000003583.12,ENST00000337248.8,ENST00000374409.5,ENST00000435187.1,ENST00000438866.1,ENST00000468303.5,ENST00000475760.1,ENST00000483261.1,ENST00000483528.1,ENST00000492753.1,ENST00000497384.1,ENST00000498488.5 942.86 867.86 26.00 2.84 2.68 26.00 0.00 4.05 4.01 2.40485 5.80664 0.146602 2.37517 5.74582 0.146662 -ENSG00000001461.16 ENST00000003912.7,ENST00000339255.2,ENST00000358028.8,ENST00000374399.8,ENST00000432012.1,ENST00000475663.2,ENST00000488155.1 4807.30 4732.30 442.00 8.87 8.37 442.00 0.00 8.89 8.80 7.76326 10.0125 0.0434186 7.69777 9.92591 0.0433995 -ENSG00000001497.16 ENST00000374804.9,ENST00000374807.9,ENST00000374811.7,ENST00000469091.1,ENST00000484069.1 2457.11 2382.11 2114.00 84.27 79.49 2114.00 0.00 79.24 78.47 75.7946 82.6864 0.0149911 75.1457 81.9595 0.0149688 -ENSG00000001561.6 ENST00000321037.4 4651.00 4576.00 0.00 0.00 0.00 0.00 0.00 0.02 0.02 2.97578e-07 0.0584334 0.659795 2.945e-07 0.0578571 0.659782 -ENSG00000001617.11 ENST00000002829.7,ENST00000413852.5,ENST00000414301.5,ENST00000420831.1,ENST00000426511.5,ENST00000434342.5,ENST00000450338.5,ENST00000470737.1,ENST00000493743.1 1434.42 1359.42 576.00 40.24 37.95 576.00 0.00 38.18 37.81 32.7025 43.8457 0.0496952 32.3373 43.3803 0.0497815 -ENSG00000001626.15 ENST00000003084.10,ENST00000426809.5,ENST00000429014.1,ENST00000446805.1,ENST00000468795.1,ENST00000472848.1,ENST00000546407.1,ENST00000600166.1,ENST00000608965.5,ENST00000610149.1,ENST00000621535.1,ENST00000647639.1,ENST00000647720.1,ENST00000647978.1,ENST00000648260.1,ENST00000649406.1,ENST00000649781.1,ENST00000649850.1 799.65 724.65 6.00 0.79 0.74 6.00 0.00 4.18 4.14 1.73303 7.1899 0.226449 1.71936 7.12216 0.226374 -ENSG00000001629.9 ENST00000265742.7,ENST00000413588.1,ENST00000422095.1,ENST00000439883.1,ENST00000442183.1,ENST00000465883.1,ENST00000486698.1 2031.23 1956.23 569.00 27.62 26.05 569.00 0.00 27.00 26.74 22.3915 31.8623 0.0591804 22.0623 31.443 0.0591459 -ENSG00000001630.16 ENST00000003100.12,ENST00000422867.1,ENST00000450723.5,ENST00000482924.1 2274.14 2199.14 430.10 18.57 17.52 419.48 9.29 17.46 17.29 15.407 19.4932 0.0404649 15.2784 19.3259 0.0404645 -ENSG00000001631.15 ENST00000340022.6,ENST00000394503.6,ENST00000394505.6,ENST00000394507.5,ENST00000412043.6,ENST00000413688.5,ENST00000422347.5,ENST00000425073.1,ENST00000425919.5,ENST00000430102.5,ENST00000433016.5,ENST00000440209.5,ENST00000444960.5,ENST00000445516.1,ENST00000452773.5,ENST00000454017.5,ENST00000458177.5,ENST00000458493.5,ENST00000466166.1,ENST00000470309.5,ENST00000475770.5,ENST00000486261.5,ENST00000487168.1,ENST00000489087.1 1529.99 1454.99 180.61 11.79 11.12 222.90 24.90 15.89 15.74 11.7206 20.0882 0.0909496 11.6055 19.8939 0.0910105 -ENSG00000002016.17 ENST00000228345.9,ENST00000358495.7,ENST00000397230.6,ENST00000430095.6,ENST00000461568.5,ENST00000463750.5,ENST00000468231.5,ENST00000481052.5,ENST00000488642.6,ENST00000535376.5,ENST00000536177.5,ENST00000541619.1,ENST00000542297.1,ENST00000542584.5,ENST00000542785.5,ENST00000543912.5,ENST00000544742.5,ENST00000545564.5,ENST00000545967.1 1447.58 1372.58 110.00 7.61 7.18 110.09 0.30 10.25 10.15 7.55721 13.111 0.0941529 7.4771 12.9775 0.0940797 -ENSG00000002079.14 ENST00000425880.1,ENST00000429079.1,ENST00000439784.7,ENST00000452010.2,ENST00000453194.2 2831.78 2756.78 125.00 4.31 4.06 125.00 0.00 4.74 4.69 3.13021 6.46812 0.125058 3.10653 6.41516 0.12506 -ENSG00000002330.13 ENST00000309032.7,ENST00000394531.3,ENST00000394532.7,ENST00000492141.1,ENST00000493798.1,ENST00000544271.1,ENST00000544785.1 537.46 462.46 32.00 6.57 6.20 32.00 0.00 8.12 8.04 4.83539 11.6023 0.145961 4.77593 11.475 0.145955 -ENSG00000002549.12 ENST00000226299.8,ENST00000503467.1,ENST00000504614.5,ENST00000507960.1,ENST00000508014.5,ENST00000508497.5,ENST00000509583.1,ENST00000512397.1,ENST00000513105.1,ENST00000606142.5,ENST00000618908.4 1095.78 1020.78 950.00 88.38 83.36 949.99 0.08 85.86 85.02 78.47 93.2937 0.0296851 77.5921 92.2696 0.0296469 -ENSG00000002586.19 ENST00000381177.7,ENST00000381180.9,ENST00000381184.6,ENST00000381187.8,ENST00000381192.9,ENST00000449611.6,ENST00000482293.6,ENST00000482405.7,ENST00000497752.7,ENST00000611428.5,ENST00000623253.4,ENST00000624481.4,ENST00000645950.1,ENST00000646103.1,ENST00000647297.1 1096.93 1021.93 455.00 42.28 39.88 455.00 0.00 43.64 43.22 38.5611 48.9815 0.041442 38.2158 48.5402 0.0414257 -ENSG00000002586.19_PAR_Y ENST00000381177.7_PAR_Y,ENST00000381180.9_PAR_Y,ENST00000381184.6_PAR_Y,ENST00000381187.8_PAR_Y,ENST00000381192.9_PAR_Y,ENST00000449611.6_PAR_Y,ENST00000482293.6_PAR_Y,ENST00000482405.7_PAR_Y,ENST00000497752.7_PAR_Y,ENST00000611428.5_PAR_Y,ENST00000623253.4_PAR_Y,ENST00000624481.4_PAR_Y,ENST00000645950.1_PAR_Y,ENST00000646103.1_PAR_Y,ENST00000647297.1_PAR_Y 825.33 750.33 0.00 0.00 0.00 0.00 0.00 2.04 2.02 0.994973 3.14845 0.185355 0.986748 3.11971 0.185332 -ENSG00000002587.9 ENST00000002596.5,ENST00000510712.1,ENST00000514690.5 8031.00 7956.00 11.00 0.13 0.12 11.00 0.00 0.56 0.55 0.122072 1.15958 0.356338 0.12335 1.15131 0.356156 -ENSG00000002726.20 ENST00000360937.8,ENST00000416793.6,ENST00000460213.1,ENST00000467291.5,ENST00000480582.1,ENST00000483043.1,ENST00000493429.5 2042.29 1967.29 0.00 0.00 0.00 0.00 0.00 0.41 0.40 0.117561 0.757859 0.280009 0.116348 0.75019 0.279875 -ENSG00000002745.12 ENST00000222462.2,ENST00000361301.6 2608.06 2533.06 3.00 0.11 0.11 3.00 0.00 0.24 0.24 0.046081 0.486859 0.342698 0.0456596 0.48223 0.342792 -ENSG00000002746.14 ENST00000395891.6,ENST00000429529.1,ENST00000453890.5,ENST00000461842.1,ENST00000464944.1,ENST00000471043.1,ENST00000471527.1,ENST00000481031.5,ENST00000490954.2,ENST00000492310.5,ENST00000493057.1 678.15 603.15 9.00 1.42 1.34 9.00 0.00 2.80 2.77 1.44875 4.28495 0.178532 1.43007 4.23864 0.17834 -ENSG00000002822.15 ENST00000265854.11,ENST00000399654.6,ENST00000402746.5,ENST00000406869.5,ENST00000421113.5,ENST00000429625.5,ENST00000429779.1,ENST00000437877.1,ENST00000438959.5,ENST00000444373.6,ENST00000445959.5,ENST00000450235.5,ENST00000455998.5,ENST00000459731.1,ENST00000464742.5,ENST00000468372.5,ENST00000469871.1,ENST00000477172.1,ENST00000481633.1,ENST00000483165.5,ENST00000486340.5,ENST00000491858.5,ENST00000496548.1 1928.44 1853.44 332.00 17.01 16.04 332.00 0.00 22.90 22.67 18.3693 27.7392 0.0708931 18.1606 27.4355 0.0707495 -ENSG00000002834.17 ENST00000318008.10,ENST00000419929.1,ENST00000433206.6,ENST00000435347.7,ENST00000443937.7,ENST00000579123.1,ENST00000581485.1,ENST00000584106.1,ENST00000585841.5 1218.65 1143.65 1531.00 127.13 119.90 1531.00 0.00 120.08 118.91 112.275 127.748 0.0223761 111.161 126.504 0.0223422 -ENSG00000002919.14 ENST00000359238.6,ENST00000393405.6,ENST00000452859.6,ENST00000577310.1,ENST00000578861.5,ENST00000580219.5,ENST00000580875.1,ENST00000581298.5,ENST00000581705.5,ENST00000582104.5,ENST00000582481.1,ENST00000583320.5,ENST00000584335.5 1147.70 1072.70 48.00 4.25 4.01 48.00 0.00 5.64 5.59 3.93119 7.40643 0.106121 3.90174 7.34292 0.106024 -ENSG00000002933.8 ENST00000004103.7,ENST00000461345.5,ENST00000462826.1,ENST00000468689.2,ENST00000474166.1,ENST00000475007.5,ENST00000475536.5,ENST00000475710.1,ENST00000481305.1,ENST00000484928.5,ENST00000494349.5 1062.82 987.82 0.00 0.00 0.00 0.00 0.00 1.19 1.18 0.507775 1.98067 0.219421 0.498569 1.95744 0.219379 -ENSG00000003056.7 ENST00000000412.7,ENST00000536844.5,ENST00000537621.1,ENST00000539143.5,ENST00000540837.1,ENST00000541507.5,ENST00000543159.1,ENST00000543258.1,ENST00000543704.5,ENST00000543834.1,ENST00000543845.1,ENST00000544193.1,ENST00000544245.1 1018.77 943.77 648.00 65.20 61.50 648.00 0.00 65.33 64.69 58.1464 72.403 0.0373942 57.6154 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ENST00000637051.1 62.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -ENSG00000283321.1 ENST00000637807.1 3523.00 3448.00 86.41 2.38 2.24 84.86 33.23 2.21 2.19 0.484192 3.97117 0.274521 0.447116 3.90214 0.274522 -ENSG00000283324.1 ENST00000636087.1 1229.00 1154.00 0.00 0.00 0.00 0.00 0.00 0.08 0.08 2.77554e-07 0.229019 0.652807 2.74896e-07 0.226782 0.652792 -ENSG00000283326.1 ENST00000635882.1 66.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -ENSG00000283327.1 ENST00000637129.1 72.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -ENSG00000283329.1 ENST00000637493.1,ENST00000637691.1 1063.00 988.00 0.00 0.00 0.00 0.00 0.00 0.18 0.18 0.00431198 0.430506 0.472009 0.00431713 0.426322 0.472205 -ENSG00000283330.1 ENST00000385014.3 87.00 12.00 0.00 0.00 0.00 0.00 0.00 7.40 7.33 4.15092e-05 22.0541 0.658923 4.11279e-05 21.8479 0.658832 -ENSG00000283333.1 ENST00000636162.1 49.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -ENSG00000283334.1 ENST00000636519.1 88.00 13.00 0.00 0.00 0.00 0.00 0.00 6.83 6.77 4.35155e-05 20.3277 0.653836 4.30301e-05 20.1433 0.653929 -ENSG00000283335.1 ENST00000637023.1 88.00 13.00 0.00 0.00 0.00 0.00 0.00 6.83 6.77 0.000652433 20.5596 0.660824 0.000203015 20.3689 0.660744 -ENSG00000283337.1 ENST00000636230.1 99.00 24.00 0.00 0.00 0.00 0.00 0.00 3.70 3.66 0.000168314 11.2248 0.658282 0.000166638 11.1231 0.658262 -ENSG00000283338.1 ENST00000636321.1 199.00 124.00 0.00 0.00 0.00 0.00 0.00 0.72 0.71 6.3223e-06 2.12257 0.658367 6.25992e-06 2.10096 0.658455 -ENSG00000283339.1 ENST00000637588.1 150.00 75.00 0.00 0.00 0.00 0.00 0.00 1.18 1.17 7.58244e-05 3.59339 0.656705 7.49892e-05 3.5553 0.656668 -ENSG00000283340.1 ENST00000637673.1 85.00 10.00 0.00 0.00 0.00 0.00 0.00 8.88 8.79 0.000170434 26.4489 0.650715 0.000168954 26.2132 0.650852 -ENSG00000283341.1 ENST00000529261.5,ENST00000532864.6,ENST00000636894.1 2134.00 2059.00 10.00 0.46 0.44 15.54 4.52 1.49 1.47 0.428615 2.93945 0.308621 0.425591 2.91135 0.308625 -ENSG00000283342.1 ENST00000637142.1 2090.00 2015.00 0.00 0.00 0.00 0.00 0.00 0.04 0.04 1.7417e-06 0.132275 0.659156 1.7221e-06 0.130993 0.659189 -ENSG00000283343.1 ENST00000636981.1 65.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -ENSG00000283345.1 ENST00000636170.1 1206.00 1131.00 7.02 0.59 0.56 3.38 2.26 0.34 0.34 3.54715e-06 0.795537 0.534549 3.51402e-06 0.78787 0.534588 -ENSG00000283346.1 ENST00000636908.1 71.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -ENSG00000283347.1 ENST00000637433.1 575.00 500.00 0.00 0.00 0.00 0.00 0.00 0.18 0.18 2.63824e-06 0.533303 0.654617 2.61069e-06 0.52845 0.654819 -ENSG00000283349.3 ENST00000636088.3,ENST00000637712.1 563.00 488.00 0.00 0.00 0.00 0.00 0.00 0.38 0.38 0.00536749 0.913252 0.476976 0.00470701 0.90394 0.477042 -ENSG00000283351.1 ENST00000636558.1 82.00 7.00 0.00 0.00 0.00 0.00 0.00 12.69 12.56 8.02227e-05 38.0044 0.653863 0.000528957 37.6527 0.653988 -ENSG00000283352.1 ENST00000636069.1,ENST00000637710.1 675.00 600.00 22.28 3.53 3.33 18.93 4.61 3.24 3.21 1.41803 5.21564 0.208647 1.40134 5.16138 0.208662 -ENSG00000283354.1 ENST00000637607.1 285.00 210.00 0.00 0.00 0.00 0.00 0.00 0.42 0.42 5.51157e-07 1.26781 0.657812 5.45785e-07 1.25604 0.657878 -ENSG00000283355.1 ENST00000636429.1 2348.00 2273.00 0.00 0.00 0.00 0.00 0.00 0.04 0.04 7.3636e-08 0.118756 0.658663 7.30154e-08 0.117657 0.658687 -ENSG00000283356.1 ENST00000637697.1 135.00 60.00 0.00 0.00 0.00 0.00 0.00 1.48 1.47 3.99385e-05 4.41486 0.651162 5.52913e-05 4.37557 0.651058 -ENSG00000283359.1 ENST00000636444.1 70.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -ENSG00000283360.1 ENST00000637155.1 863.00 788.00 0.00 0.00 0.00 0.00 0.00 0.11 0.11 9.23552e-07 0.336053 0.656925 9.16433e-07 0.332792 0.65686 -ENSG00000283361.2 ENST00000635874.1,ENST00000635901.2,ENST00000635974.1,ENST00000636692.1,ENST00000643687.1,ENST00000646158.1 735.50 660.50 0.00 0.00 0.00 0.00 0.00 0.97 0.96 0.249109 1.79945 0.290815 0.246348 1.7816 0.290738 -ENSG00000283364.1 ENST00000636285.1 68.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 0 0 0 0 0 -ENSG00000283365.1 ENST00000636915.1 979.00 904.00 0.00 0.00 0.00 0.00 0.00 0.10 0.10 2.88131e-06 0.291492 0.654055 2.85043e-06 0.288605 0.654144 -ENSG00000283366.2 ENST00000637415.1 154.00 79.00 0.00 0.00 0.00 0.00 0.00 1.12 1.11 2.19362e-05 3.35892 0.654343 2.17092e-05 3.32806 0.654323 -ENSG00000283367.1 ENST00000636724.1 160.00 85.00 0.00 0.00 0.00 0.00 0.00 1.04 1.03 2.38663e-05 3.1473 0.658276 2.36377e-05 3.11883 0.658267 -ENSG00000283369.1 ENST00000637707.1 76.00 1.00 0.00 0.00 0.00 0.00 0.00 88.81 87.95 0.000619287 267.217 0.653828 0.00061105 264.677 0.6538 -ENSG00000283371.1 ENST00000636373.1 85.00 10.00 0.00 0.00 0.00 0.00 0.00 8.88 8.79 0.000130901 26.5501 0.654845 0.000129793 26.2999 0.654903 -ENSG00000283372.1 ENST00000637737.1 98.00 23.00 0.00 0.00 0.00 0.00 0.00 3.86 3.82 0.000264952 11.5251 0.655274 0.000262412 11.4171 0.655218 -ENSG00000283375.1 ENST00000637427.1 289.00 214.00 0.00 0.00 0.00 0.00 0.00 0.42 0.41 1.54096e-05 1.24335 0.655319 1.52722e-05 1.23215 0.655213 -ENSG00000283376.1 ENST00000636637.1 786.00 711.00 0.00 0.00 0.00 0.00 0.00 0.12 0.12 9.80023e-07 0.372767 0.659178 9.71091e-07 0.369254 0.659398 -ENSG00000283377.1 ENST00000636779.1 956.00 881.00 0.00 0.00 0.00 0.00 0.00 0.10 0.10 1.03268e-06 0.300538 0.65862 1.02539e-06 0.297716 0.658745 -ENSG00000283378.1 ENST00000636699.1 1728.00 1653.00 0.00 0.00 0.00 0.00 0.00 0.05 0.05 3.47154e-07 0.161493 0.654211 3.43603e-07 0.159942 0.654142 -ENSG00000283379.1 ENST00000636963.1 87.00 12.00 0.00 0.00 0.00 0.00 0.00 7.40 7.33 8.11438e-05 22.0322 0.661084 8.02372e-05 21.8095 0.661011 -ENSG00000283380.1 ENST00000636317.1,ENST00000636450.1,ENST00000636678.1,ENST00000637444.1,ENST00000637843.1 1574.40 1499.40 0.00 0.00 0.00 0.00 0.00 0.36 0.35 0.0696783 0.693733 0.32453 0.0720077 0.690012 0.324428 -ENSG00000283381.1 ENST00000637860.1 256.00 181.00 0.00 0.00 0.00 0.00 0.00 0.49 0.49 2.56829e-06 1.45313 0.656116 2.54719e-06 1.44005 0.656067 -ENSG00000285984.1 ENST00000649302.1 805.00 730.00 0.00 0.00 0.00 0.00 0.00 0.12 0.12 1.89756e-07 0.364533 0.659123 1.878e-07 0.361058 0.659025 -ENSG00000285985.1 ENST00000650218.1 1458.00 1383.00 0.00 0.00 0.00 0.00 0.00 0.06 0.06 2.51271e-06 0.192837 0.65919 2.48685e-06 0.191067 0.659182 -ENSG00000285986.1 ENST00000649395.1 614.00 539.00 0.00 0.00 0.00 0.00 0.00 0.16 0.16 4.24778e-07 0.494016 0.65782 4.21029e-07 0.48901 0.657837 -ENSG00000285987.1 ENST00000650453.1 3205.00 3130.00 0.00 0.00 0.00 0.00 0.00 0.03 0.03 1.32221e-06 0.0844541 0.6551 1.30962e-06 0.0836267 0.655159 -ENSG00000285988.1 ENST00000648995.1 614.00 539.00 2.63 0.46 0.44 1.71 1.41 0.45 0.44 5.48494e-05 1.14506 0.573189 5.42967e-05 1.1345 0.573037 -ENSG00000285989.1 ENST00000649196.1 303.00 228.00 0.00 0.00 0.00 0.00 0.00 0.39 0.39 9.61392e-07 1.16559 0.654349 9.53238e-07 1.15366 0.654071 -ENSG00000285990.1 ENST00000649331.1 647.00 572.00 0.00 0.00 0.00 4.95 4.72 0.92 0.92 2.27226e-05 2.53767 0.613634 2.25162e-05 2.51213 0.613727 -ENSG00000285991.1 ENST00000647612.1 5065.00 4990.00 237.77 4.52 4.27 226.77 33.25 4.05 4.01 2.80774 5.34539 0.109439 2.77428 5.28871 0.10957 -ENSG00000285992.1 ENST00000648949.1 956.00 881.00 0.00 0.00 0.00 0.00 0.00 0.10 0.10 3.97032e-06 0.302472 0.656639 3.92936e-06 0.299708 0.656873 -ENSG00000285993.1 ENST00000650266.1 1246.00 1171.00 0.00 0.00 0.00 0.00 0.00 0.08 0.08 1.14779e-06 0.22985 0.655948 1.13746e-06 0.227736 0.655816 -ENSG00000285994.1 ENST00000648650.1 3732.00 3657.00 2.00 0.05 0.05 2.00 0.00 0.07 0.07 0.00832538 0.157048 0.390303 0.00772234 0.15499 0.390257 -gSpikein_ERCC-00002 tSpikein_ERCC-00002 1061.00 986.00 0.00 0.00 0.00 0.00 0.00 0.09 0.09 1.97043e-07 0.272584 0.661995 1.94914e-07 0.270118 0.662061 -gSpikein_ERCC-00003 tSpikein_ERCC-00003 1023.00 948.00 0.00 0.00 0.00 0.00 0.00 0.09 0.09 2.17459e-06 0.280274 0.657516 2.15348e-06 0.277411 0.657529 -gSpikein_ERCC-00004 tSpikein_ERCC-00004 523.00 448.00 0.00 0.00 0.00 0.00 0.00 0.20 0.20 2.47105e-06 0.598761 0.655987 2.44203e-06 0.59303 0.656021 -gSpikein_ERCC-00007 tSpikein_ERCC-00007 1135.00 1060.00 0.00 0.00 0.00 0.00 0.00 0.08 0.08 6.04506e-07 0.250011 0.655578 5.98306e-07 0.247508 0.655403 -gSpikein_ERCC-00009 tSpikein_ERCC-00009 984.00 909.00 0.00 0.00 0.00 0.00 0.00 0.10 0.10 1.51006e-06 0.292478 0.659334 1.49343e-06 0.289717 0.659433 -gSpikein_ERCC-00012 tSpikein_ERCC-00012 994.00 919.00 0.00 0.00 0.00 0.00 0.00 0.10 0.10 7.86361e-06 0.289432 0.654324 7.77686e-06 0.28671 0.654644 -gSpikein_ERCC-00013 tSpikein_ERCC-00013 808.00 733.00 0.00 0.00 0.00 0.00 0.00 0.12 0.12 6.70321e-07 0.365201 0.652607 6.64453e-07 0.361745 0.652494 -gSpikein_ERCC-00014 tSpikein_ERCC-00014 1957.00 1882.00 0.00 0.00 0.00 0.00 0.00 0.05 0.05 1.21967e-06 0.139452 0.653567 1.20664e-06 0.138062 0.653703 -gSpikein_ERCC-00016 tSpikein_ERCC-00016 844.00 769.00 0.00 0.00 0.00 0.00 0.00 0.12 0.11 4.67955e-06 0.34575 0.653338 4.63172e-06 0.342477 0.653545 -gSpikein_ERCC-00017 tSpikein_ERCC-00017 1136.00 1061.00 0.00 0.00 0.00 0.00 0.00 0.08 0.08 9.84285e-06 0.250313 0.658236 4.03221e-06 0.247862 0.658317 -gSpikein_ERCC-00018 tSpikein_ERCC-00018 1032.00 957.00 0.00 0.00 0.00 0.00 0.00 0.09 0.09 8.60373e-07 0.278409 0.655427 8.53433e-07 0.275653 0.655519 -gSpikein_ERCC-00019 tSpikein_ERCC-00019 644.00 569.00 0.00 0.00 0.00 0.00 0.00 0.16 0.15 7.72849e-06 0.47193 0.655629 7.63883e-06 0.467266 0.655576 -gSpikein_ERCC-00022 tSpikein_ERCC-00022 751.00 676.00 0.00 0.00 0.00 0.00 0.00 0.13 0.13 1.11469e-05 0.392739 0.657401 1.10554e-05 0.389013 0.657654 -gSpikein_ERCC-00023 tSpikein_ERCC-00023 273.00 198.00 0.00 0.00 0.00 0.00 0.00 0.45 0.44 3.64833e-06 1.33762 0.651104 3.61442e-06 1.32409 0.651055 -gSpikein_ERCC-00024 tSpikein_ERCC-00024 536.00 461.00 0.00 0.00 0.00 0.00 0.00 0.19 0.19 3.43121e-06 0.575688 0.657792 3.39641e-06 0.569951 0.658012 -gSpikein_ERCC-00025 tSpikein_ERCC-00025 1994.00 1919.00 0.00 0.00 0.00 0.00 0.00 0.05 0.05 4.52746e-07 0.138973 0.658385 4.47835e-07 0.137588 0.658559 -gSpikein_ERCC-00028 tSpikein_ERCC-00028 1130.00 1055.00 0.00 0.00 0.00 0.00 0.00 0.08 0.08 1.8974e-06 0.251691 0.660308 1.87886e-06 0.249292 0.660199 -gSpikein_ERCC-00031 tSpikein_ERCC-00031 1138.00 1063.00 0.00 0.00 0.00 0.00 0.00 0.08 0.08 1.96138e-06 0.250887 0.660078 1.94173e-06 0.248442 0.659976 -gSpikein_ERCC-00033 tSpikein_ERCC-00033 2022.00 1947.00 0.00 0.00 0.00 0.00 0.00 0.05 0.05 8.43184e-07 0.136088 0.656291 8.33761e-07 0.134776 0.656345 -gSpikein_ERCC-00034 tSpikein_ERCC-00034 1019.00 944.00 0.00 0.00 0.00 0.00 0.00 0.09 0.09 3.09662e-06 0.279717 0.65568 3.06876e-06 0.27695 0.65555 -gSpikein_ERCC-00035 tSpikein_ERCC-00035 1130.00 1055.00 0.00 0.00 0.00 0.00 0.00 0.08 0.08 2.1958e-06 0.253023 0.654874 2.17406e-06 0.250608 0.654797 -gSpikein_ERCC-00039 tSpikein_ERCC-00039 740.00 665.00 0.00 0.00 0.00 0.00 0.00 0.13 0.13 2.5224e-06 0.398666 0.653763 2.49525e-06 0.394954 0.653803 -gSpikein_ERCC-00040 tSpikein_ERCC-00040 744.00 669.00 0.00 0.00 0.00 0.00 0.00 0.13 0.13 5.49455e-06 0.399104 0.656637 5.44449e-06 0.394957 0.656615 -gSpikein_ERCC-00041 tSpikein_ERCC-00041 1122.00 1047.00 0.00 0.00 0.00 0.00 0.00 0.08 0.08 8.09206e-07 0.253786 0.655282 8.01581e-07 0.251356 0.655334 -gSpikein_ERCC-00042 tSpikein_ERCC-00042 1023.00 948.00 0.00 0.00 0.00 0.00 0.00 0.09 0.09 1.54543e-06 0.27656 0.655019 1.52891e-06 0.274076 0.654969 -gSpikein_ERCC-00043 tSpikein_ERCC-00043 1023.00 948.00 0.00 0.00 0.00 0.00 0.00 0.09 0.09 1.14581e-06 0.280704 0.652402 1.13396e-06 0.277896 0.65252 -gSpikein_ERCC-00044 tSpikein_ERCC-00044 1156.00 1081.00 0.00 0.00 0.00 0.00 0.00 0.08 0.08 1.6728e-06 0.245831 0.66018 1.65348e-06 0.243524 0.660096 -gSpikein_ERCC-00046 tSpikein_ERCC-00046 522.00 447.00 0.00 0.00 0.00 0.00 0.00 0.20 0.20 6.77956e-06 0.594022 0.656114 6.71853e-06 0.588503 0.655938 -gSpikein_ERCC-00048 tSpikein_ERCC-00048 992.00 917.00 0.00 0.00 0.00 0.00 0.00 0.10 0.10 3.0881e-06 0.292147 0.657351 3.05993e-06 0.289314 0.657339 -gSpikein_ERCC-00051 tSpikein_ERCC-00051 274.00 199.00 0.00 0.00 0.00 0.00 0.00 0.45 0.44 3.52807e-06 1.34813 0.655986 3.5012e-06 1.335 0.656028 -gSpikein_ERCC-00053 tSpikein_ERCC-00053 1023.00 948.00 0.00 0.00 0.00 0.00 0.00 0.09 0.09 5.73911e-07 0.281243 0.659662 5.68993e-07 0.278599 0.659716 -gSpikein_ERCC-00054 tSpikein_ERCC-00054 274.00 199.00 0.00 0.00 0.00 0.00 0.00 0.45 0.44 4.24762e-06 1.3374 0.651083 4.20781e-06 1.32393 0.65112 -gSpikein_ERCC-00057 tSpikein_ERCC-00057 1021.00 946.00 0.00 0.00 0.00 0.00 0.00 0.09 0.09 1.92855e-06 0.283017 0.654324 1.90478e-06 0.28028 0.654323 -gSpikein_ERCC-00058 tSpikein_ERCC-00058 1136.00 1061.00 0.00 0.00 0.00 0.00 0.00 0.08 0.08 1.11134e-06 0.251129 0.653592 1.10083e-06 0.248741 0.653655 -gSpikein_ERCC-00059 tSpikein_ERCC-00059 525.00 450.00 0.00 0.00 0.00 0.00 0.00 0.20 0.20 2.60162e-06 0.595965 0.658761 2.57866e-06 0.590109 0.658887 -gSpikein_ERCC-00060 tSpikein_ERCC-00060 523.00 448.00 0.00 0.00 0.00 0.00 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tSpikein_ERCC-00074 522.00 447.00 0.00 0.00 0.00 0.00 0.00 0.20 0.20 1.53073e-05 0.596343 0.657507 2.55191e-05 0.590958 0.657555 -gSpikein_ERCC-00075 tSpikein_ERCC-00075 1023.00 948.00 0.00 0.00 0.00 0.00 0.00 0.09 0.09 1.71248e-06 0.284013 0.657298 1.6957e-06 0.281195 0.65717 -gSpikein_ERCC-00076 tSpikein_ERCC-00076 642.00 567.00 0.00 0.00 0.00 0.00 0.00 0.16 0.16 5.80788e-06 0.46897 0.653784 1.65474e-05 0.464668 0.653924 -gSpikein_ERCC-00077 tSpikein_ERCC-00077 273.00 198.00 0.00 0.00 0.00 0.00 0.00 0.45 0.44 9.49773e-06 1.35995 0.656777 9.41326e-06 1.34612 0.656635 -gSpikein_ERCC-00078 tSpikein_ERCC-00078 993.00 918.00 0.00 0.00 0.00 0.00 0.00 0.10 0.10 4.39877e-06 0.289476 0.655742 4.36333e-06 0.286754 0.655788 -gSpikein_ERCC-00079 tSpikein_ERCC-00079 644.00 569.00 0.00 0.00 0.00 0.00 0.00 0.16 0.15 5.86565e-07 0.467344 0.656923 5.82383e-07 0.462721 0.656883 -gSpikein_ERCC-00081 tSpikein_ERCC-00081 534.00 459.00 0.00 0.00 0.00 0.00 0.00 0.19 0.19 6.07311e-06 0.58179 0.654412 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5739.05 2790.81 1047.43 0 0 3.51 0 3633.86 4047.48 1.09 0.77 0 64.07 1.88 4.1 808.48 1771.98 0 59.68 0 0 2.25 0.55 12.67 5345.75 1290.92 5.04 541.45 909.51 6995.27 2.89 21.47 17.35 436.03 551.16 179.05 21.47 1859.12 805.53 96.15 724.21 3218.17 3.58 305.62 0 0 0 1382.24 2100.28 1371.04 1101.83 496.16 189.92 720.91 1466.47 2047.76 1804.36 1969.32 1298.83 1002.8 2165.22 2177.22 1946.86 1285.32 772.15 1932.87 553.09 3427.18 2319.43 1264.55 180.79 1130.1 2003.27 1310.9 1445.01 1216.03 1264.19 969.11 408.7 1321.8 488.03 1059.57 2791.78 647.98 870.12 1005.59 1338.81 1209.65 885.01 2628.31 2091.89 14.29 855.54 933.89 2868.87 3764.46 1824.06 0.56 61.95 1374.95 2510.23 +ERCC-00024 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 +ERCC-00025 0 0 0 0 0 0 0 0 0 0 0 0 0 6.01 0.76 10.58 4.74 71.48 60.12 81.77 22.76 14.31 16.97 15.17 5.98 58.84 43.46 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 117.29 0 0 0 0 0 1921.2 2351.45 167.38 1423.32 599.66 0 389.3 214.68 1.02 942 0 0 0 0 11.26 361.52 0 0 5.9 42.6 0 10.07 0.59 1298.22 0 0 0 0 0 0 0 2847.16 1.41 594.39 12.62 99.15 0 0 0.21 0 0 0.23 0 0.2 0 0.1 0.16 0.69 3.8 50.86 988.17 6.74 6.71 448.36 1.73 0 1.44 27.98 184.75 0.32 2219.81 188.85 294.94 13.7 1.44 1.07 1.1 74.09 0.06 0 0 248.31 227.11 459.03 418.93 337.23 470.55 467.15 63.87 57.94 68.93 63.98 43.01 92.04 68.44 72.79 83.44 595.35 91.58 270.95 1312.89 549.23 382.66 143.73 242.63 246.73 359.72 132.82 103.97 114.32 101.03 108.18 301.64 113.45 142.41 364.98 233.71 297.53 612.9 379.71 691.7 307.57 461.91 200.54 263.93 431.1 71.99 61.63 1709.57 14.6 56.91 0.16 892.94 1.26 450.94 +ERCC-00028 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.89 0 0 0 0 0 0 4.12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30.31 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 563.42 0 0 0 0 0 0 0.73 0 0 0 0 0 0 0.1 0 0 0 1.75 28.48 0 6.56 20.83 0 10.37 1.67 1.15 4.83 1.97 1.4 1.97 1.28 1.43 1.87 0 0 0 370.82 1.71 0 0 0 0 0 2.14 1.34 5.04 3.33 7.33 2.07 4.29 52.7 0.74 0.48 0 0 0 257.59 0 4.91 44.86 0 0 0 2.48 1.58 1.31 0 0 0.61 0 0 +ERCC-00031 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 842.27 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.35 17.66 23.86 1.5 0 35.99 0.41 0 0 0.56 0.35 1.96 0.95 0.28 0.46 0.63 0 0 0 0.85 0 0 0 0 0 1.06 1.33 0 0.41 1.28 13 1.06 0.43 0 0 28.81 0 4.7 7.12 3.98 0 0.91 0 0 0 0.82 0 0.33 0 0 0 0 0 +ERCC-00033 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.36 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.35 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.72 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 +ERCC-00034 0 0 0 0 0 0 0 0 0 0 0 0 0 0 22.69 34.48 0 82.33 48.91 0 0 0 7.93 0 0.14 8.74 0 0 0 36.58 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 220.62 0 0 0 0 0 22.88 125.13 12.02 0 0 956.35 0 0 0 0 5154.24 0 0 0 0 0 0 0 0 1051.56 0 0 0 0 117.94 0 2564.73 0 0 0 0 0 0.77 0 0 0 1.65 0.61 1.37 0.6 0 0 0 2.66 0 0 0 0 9.07 4.41 0.38 0 0.35 0.38 0 0 0.39 0 0 0 0.21 0 0 0 0 0 1.83 150.34 0 0 0 80.48 115.8 247.75 142.2 119.79 90.27 90.4 23.72 23.48 25.95 26.55 15.07 33.64 23.2 28.2 29.12 172.35 221.07 0 0 9.24 0.39 26.39 1137.15 258.42 626.07 23.06 37.21 37.66 20.78 35.75 77.69 38.22 1.49 240.03 215.54 351.56 279.69 102.66 1.64 0 109.18 6.62 46.37 0 594.32 55.57 2.69 4573.51 1.17 0 0.7 384.84 5.91 +ERCC-00035 0 0 0 0 0 0 0 0 0 0 0 0.14 0.12 0 82.87 6.77 0 66.07 80.16 23.3 19.73 16.23 101.97 0 158.37 14.3 41.09 2.29 0 0.16 0 0 324.77 0 0 0 0 0 0 0 0 0 0 0 0 91.24 0 0 0 0 0 2414.52 183.49 153.54 924.39 1741.8 0 13.44 0 0 0 37.96 65.31 5014.93 3543.5 281.57 0 812.29 4735.98 34.02 1346.44 4059.3 0 146.84 1.23 0 0 0 0.66 4156.93 2.05 0.84 9199.12 2.04 9.86 28.85 2.56 0 0 0 0 4933.65 0.23 0.41 0.78 0.59 0.75 0 1448.64 539.19 14.72 3413.23 0 2.17 1691.95 2.11 740.35 273.82 0.82 3.94 125.74 931.66 1119.06 12.29 10631.51 10.22 2822.33 1.3 56.4 0.13 0 0 427.35 588.66 369.54 844.03 442.26 1454.5 509.27 199.69 190.07 260.6 259.54 166.32 197.34 265.4 259.8 279.68 276.76 541.7 124.53 829.94 84.17 2.4 218.79 321.04 829.21 525.51 242.98 319.25 231.71 254.5 236.34 328.53 239.94 365.42 722.2 359.06 569.41 1211.76 278.01 790.48 845.56 496.27 172.43 598.2 2.98 220.59 130.52 777.63 81.46 89.38 200.1 1062.83 4.27 17.39 +ERCC-00039 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11.14 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7.36 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4.31 0 0 0 0 0 0 0 0 0 0 0 0 0 16.59 112.64 0 69.62 40.89 66.13 28.83 1.44 1.95 8.18 4.82 0.6 2.05 4.41 2.47 4.79 0 0 0 0 1.49 0 0 0.79 4.39 0 1.83 6.83 3.43 3.55 1.49 4.19 3.72 0 204.44 26.45 1.59 0 0 2.52 0.54 0 20.51 0 1.04 0 2.91 1.36 0.58 0 0 0 0.64 0 +ERCC-00040 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13.72 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.94 4.56 0 0 0 0 0.71 0 0 1.43 0 0 0.55 0.49 1.58 0 0 1.64 0 0.74 0 0 0 0 0 0.91 1.13 0 1.41 0 0 0 0 0 0 22.04 0 0 0 0 3.58 1.07 0 0 0 4.33 0 0 0 0 0 0 0 +ERCC-00041 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11.3 0 0 0 0 0 0 0 0 0 0 0 0 0.29 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 +ERCC-00042 0 0 0 0 0 0 0 0 0 0 0 0 0.79 54.03 308.52 383.74 442.83 428.26 533.04 610.34 480.02 874.23 349.14 47.8 256.58 513.39 225.33 11.15 12.92 15.19 61.42 184.51 308.81 0 0 0 0 0 0 0 0 0.21 0 0 0 1012.49 0 0 0 0 0 3539.23 7387.88 335.06 43.59 313.09 3580.93 531.93 0 469.1 176.25 6497.9 147.24 2074.86 314.44 3374.72 4485.01 688.98 573.63 2469.81 1013.94 10587.56 13151.19 3150.88 4928.06 7457.89 6258.08 1744.57 2928.43 3252.62 9.28 2650.69 6595.39 2421.13 3229.62 5109.26 7124.84 1553.55 546.68 12.31 7635.68 11004.71 1435.16 2940.32 1358.61 3 1850.03 3497.81 2297.89 3886.18 7106.61 1771.97 1765.95 247.58 2938.5 4.78 1643.93 161.52 2921.13 580.43 4914.37 2527.03 1687.83 1615.54 5086.45 2926.16 323.7 3.33 684.34 0 0.11 0 3921.52 4498.76 4657.91 3602.14 4127.15 2876.75 5960.83 4366.11 5443.4 2711.82 2268.13 4229.14 5368.44 4136.45 3172.54 4193.1 6868.17 5640.52 1156.39 1976.23 2059.95 4784.39 4482.49 4384.05 5619.44 6649.44 4576.54 3807.06 4447.62 2982.52 5817.19 4335.62 4376.13 3031.68 2653.49 1769.71 4078.45 2536.32 3614.55 4916.4 4564.45 4386.39 4923.12 4733.89 3640.29 7606.54 3510.52 774.42 5114.67 1907.66 7998.22 6393.34 3054.31 3723.55 +ERCC-00043 0 0 0 0 0 0 0 0 0 0 0 0 1.58 48.79 588.82 355.94 512.82 644.29 781.12 649.84 524.63 795.46 535.93 17.19 643.46 343.91 177.85 19.91 32.66 265.96 9.77 286.19 474.7 0 0 0 0 0 0 0 0 0 0 0 0 898.84 0 0 0 0 0 1183.54 1245.15 143.6 29.06 82.88 601 60.79 0 772.25 1246.95 1175.61 3190.15 0 161.99 3215.76 1732.36 0 573.63 1535.63 3894.59 0 4588.81 0.98 2385.26 0 4140.46 0 1300.48 4111.04 1187.66 12.34 33.66 127.57 2250 2451.98 301.06 3374.63 15.15 701.81 0.9 7668.53 0.26 7166.87 354.67 0 9104.3 402.48 424.3 179.23 217.75 428.09 1.51 33.62 68.57 0 138.05 21.8 172.65 115.88 458.07 122.31 301.6 22.35 160.68 30.98 0 9.7 601.46 0.15 0.11 0 694.62 1046.15 664.14 744.04 1009.71 138.73 1001.92 2107.52 2273.55 1208.43 1302.12 2631.96 1502.66 2189.61 2019.44 2008.03 1564.29 286.43 388.32 286.09 722.63 505.19 906.4 868.21 917.49 489.86 1887.01 863.9 1032.4 1319.04 1854.91 645.81 1527.36 80.4 608.92 344.82 915.7 353.04 662.39 468.51 589.3 785.92 608.85 962.58 373.09 263.3 120.89 10.1 168.8 1409.01 2554.9 223.57 331.05 107.05 +ERCC-00044 0 0 0 0 0 0 0 0 0 0 0 0 5.4 0.28 76.9 247.68 70.46 108.45 48.44 10.26 45.83 92.97 45.21 0.36 76.54 95.77 99.15 3.77 0 0 22.09 36.7 52.02 0 0.28 0 0 0 0 0 0 0 0 0 0 127.06 0 0 0 0 0 301.81 178.47 20.6 12.49 95.35 0 0 0 1557.79 383.54 0 21.19 618.59 0 1159.05 64.08 1382.61 370.19 12434.31 2642.48 179.84 5.2 0 1.2 150.65 0.36 1375.63 0 0 0 0 13.82 0 0 11.21 0 1.68 1.55 0 1.54 3016.76 0.44 66.69 0.38 5152.47 0.55 3344.4 3662.78 4712.94 1635.18 133.28 4395.37 830.08 1.96 4.92 1453.48 166.78 0.8 1.22 0 1.77 336.78 1731.1 307.05 603.94 8569.08 1.51 80.83 0 0 0 919.49 1023.67 1148 1460.77 907.6 1654.92 997.62 307.24 361.06 379.33 381.04 245.11 371.6 405.52 375.28 425.26 1433.84 979.96 169.67 1244.41 885.31 675.3 593.82 1933.02 416.35 1169.3 552.05 645.65 571.64 520.06 558.82 912.22 491.73 937.63 522.32 1487.52 485.64 1281.54 1366.75 735.86 1083.51 938.87 1004.58 726.34 4.64 841.81 236.07 8871.96 1512.3 1487.93 1092.97 773.71 89.47 3.96 +ERCC-00046 0 0 0 0 0 0 0 0 0 0 0 25.98 1.49 247.99 3913.57 5343.99 6367.05 4833.85 7537.6 6251.29 5222.28 10540.42 5024.76 296.88 6394.97 5504.52 2130.02 97.6 1006.61 1614.82 278.18 2179.56 4048.84 0 0 0 0.69 0 0.55 0 508.19 0.54 0.47 307.32 0 8753.27 0 0 0 0 0 10859.61 6003.58 657.29 832.33 1007.79 6345.68 1409.26 1318.26 2409.53 13086.39 11282.82 10797.81 11951.26 2395.65 9870.74 10484.36 28348.94 14702.71 21466.24 8696.65 27168.77 5174.02 25116.54 52562.96 187299.32 199891.27 29645.65 4271.71 69445.5 4242.21 1949.66 3756.02 2801.02 43509.62 7140.28 10503.48 2889.69 187.28 25961.21 34193.75 84008.45 54829.18 13726.99 2142.55 79.19 22062.6 10180.54 32041.09 21835.52 49497.52 23708.11 12274.3 7504.94 14936.93 6058.94 2341.62 2267.72 23298.02 15037.68 39940.03 3380.89 27460.54 9029.26 23088.32 19883.12 5232.86 34.99 6498.23 21.66 0.81 0.27 10106.82 6486.45 2792.47 2048.17 16857.46 764.13 5587.94 108803.84 99291.57 61365.39 53698.28 128176.33 29447 69549.73 73938.29 85264.23 48666.58 14300.39 23911.73 16720.08 45567.09 7639.79 15294.65 22958.32 14159.74 26172.54 46232.84 29196.97 31004.13 31768.86 48030.58 7947.91 30382.09 172.65 25323.81 21185.96 6457.45 2864.39 18806.4 2243.16 11829.55 3984.47 4740.23 6983.09 23674.82 39485.72 20083.59 8838.23 22380.28 61219.51 14610.11 15485.96 9007.68 39425.91 +ERCC-00048 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.65 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 +ERCC-00051 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30.89 36.69 25.73 29.38 10.21 47.36 0 44.33 71.41 0 10.74 11.41 9.23 1.98 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 20.98 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1546.6 0 0 0 11.42 0 0 0 0 255.78 131.92 11.68 0 39.02 0 0 0 3.83 0 0 0 0 0 0 46.55 0 0 0 0 0 84.73 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 32.81 0 0 0 20.53 0 0 0 0 0 0 0 0 0 0 119.6 322.02 196.66 63.59 190.35 17.87 182.89 156.9 115.96 0 0 0 0 0 0 7.45 0 0 0 0 45.58 9.98 25.48 52.04 0 8.38 0 0 0 0 0 0 0 0 0 0 0 36.82 12.75 0 0 0 0 0 0 0 0 +ERCC-00053 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.66 2.16 10.29 0 4.66 106.36 5.38 0 0 0 87.29 0.41 9.5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.62 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 16.84 0 0 0 130.36 311.99 0 0 0 0 0 0 88.92 0 0 0 0 0 0 0 8.37 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.61 0 0 0 0 0 0 0 0 0 0 0 0.72 0 0 0 0.49 0.53 0 0.62 0.86 0 0.73 1.42 1.3 0 0.64 3.16 3.57 2.89 2.58 2.63 0 0 0 2.8 0.48 0 35.21 14.25 0 5.81 3.02 0 0 1.41 1.95 2.34 0 0 0.84 0.54 2.06 0.68 11.52 0 1.4 2.32 0.35 0 0 3.68 0 0 0 0 0 0 0 0 +ERCC-00054 0 0 0 0 0 0 0 0 0 0 0 0 0 0 21.62 0 0 0 0 0 0 79.79 0 0 0 0 0 0 0 0 28.59 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11.42 0 0 0 0 0 0 0 0 0 0 0 5.17 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100.34 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9.2 0 0 0 0 0 3.66 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 +ERCC-00057 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.36 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 328.41 0 0 0 0 0 0 0 0 0 0 0 0 0 +ERCC-00058 0 0 0 0 0 0 0 0 0 0 0 0 0 0 31.24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 105.19 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13.91 13.92 0 0 16.18 0 11.59 0 0 0 2.8 0 1.57 0.63 0.85 0 0 0 0 0 1.7 0 0 0 0 0.39 1.6 0.67 0 0 0.43 2.4 1.78 0 0 0 0 0 11.1 0 125.52 0 0.91 0 0 0 1.64 0.26 0.33 0 0 0 0 0 +ERCC-00059 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.83 0 0 18.44 0 0 95.13 0 0.78 55.35 24.7 0 0 1.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12447.26 318.62 15.15 0 0 0 0 0 0 0 0 0 0 0 183.41 0 0 0 0 302.45 0 0 0 0 1.74 0 0 0 0 0 0 0 0 0 0 0 0 464.13 24.54 20.85 13.29 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9.7 0 0 0 0 0 0 0 0 0 0.35 0 0 0 1.25 59.37 5.81 9.35 3.34 4.3 2.87 63.37 80.77 129.14 59.2 38.69 53.54 93.09 87.52 96.97 3.65 0 195.3 760.84 46.77 0 1.64 441.97 22.72 734.82 50.93 36.93 39.05 34.91 23.44 19.08 16.66 5.14 448.84 353.3 0 111.62 318.28 8.61 358.66 6.19 2.8 113.23 533.8 0 89.81 1862.37 19.83 4.67 589.25 0.95 5.6 4.47 +ERCC-00060 0 0 0 0 0 0 0 0 0 0 0 0 0 47.74 130.19 280.77 201.99 85.62 205.64 371.59 361.34 390.9 181.87 29.46 271.9 207.25 96.47 3.07 3.61 0 0 387.07 796.04 0 0 0 0 0 0 0 0 0.54 0 0 0 659.84 0 0 0 0 0 404.28 0 91.43 263.99 137.02 599.19 0 0 346.34 210.44 0 0 3254.19 36.56 1599.17 0 0 122.14 32.04 644.2 1.27 7.09 11.25 365.23 408.43 1249.36 11.7 469.26 1693.56 63.39 0 97.67 132.21 30.25 0 203.64 450.28 70.2 57.63 1527.78 30.24 531.86 3.2 127.6 0 29.87 130.28 86.98 109.62 364.75 60.11 100.68 61.46 155.63 391.22 83.5 32.16 100.11 57.29 46.07 145.46 319.11 95.56 31.18 73.59 9.26 2.11 477.95 2.16 0.27 0 13.89 29.86 9.75 7.84 28.03 0 52.95 879.37 1140.53 1075.74 1200.6 1233.68 286.56 1511.8 1374.98 963.67 11.03 122.92 159.55 122.96 110.69 4.17 146.8 264.48 139.12 81.26 336.06 310.18 252.49 391.03 331.47 71.75 470.55 12.94 4.32 60.62 10.84 35.71 77.79 7.22 61.73 28.68 100.52 72.27 0 366.32 27.62 135.15 59.9 145.07 391.2 932.11 67.69 85.41 +ERCC-00061 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 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0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 26.62 15.33 2.81 60.59 0 0 1.38 0 1.18 0 4.67 1.46 0 5.38 0.6 1.93 0 381.73 0 0.75 3.65 0 0 0.49 0 0 2.21 0 0 1.72 0 4.38 0 0 0 0 0 68.28 0 0 0 176.51 9.32 0 0 0 5.36 3.31 0.71 0 0 0.68 0 0 +ERCC-00069 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 32.74 0 0 0 0 0 0 0.26 0 0 0 0 4.78 15.29 34.71 3.8 4.13 0 72.38 0 0 1.2 1.4 1.73 0.78 2.22 0.28 1.39 0 0 0 0.35 2.97 0 0 0 0 24.22 0.53 5.33 4 1.24 2.14 25.34 4.26 0 0 192.74 0 0 0 0 5.82 4.87 0 98.22 0 0 6.56 0.26 0.33 0.25 0 0 0 0 +ERCC-00071 0 0 0 0 0 0 0 0 0 0 0 0 0 0 34.02 10.49 0 19.38 0.92 15.14 0 181.8 137.99 0 34.29 100.5 62.06 0 97.83 0 56.41 0 83.19 0 0 0 0 0 0 0 0 0 0 0 0 382.2 0 0 0 0 0 66.13 0 11.14 0 0 0 0 0 0 0 0 0 38.37 0 12258.88 8735.94 0 89.24 70.82 1238.75 9019.33 0 0 0.66 0 4378.65 0 0 0 0 0 0 0 2522.32 0 0 49.4 58.8 0 1.13 0.42 0.5 0.46 0 0.64 1.22 0.7 0 1.19 0 0.7 254.07 3893.61 0.72 10.01 370.74 3.59 2908.72 3020.81 0 0.39 1683.39 12.39 173.77 53.8 2.23 2.07 262.9 0 0 0 298.75 355.08 94.49 172.27 150.76 88.03 45.88 260.32 345.55 346.65 377.84 248.18 136.96 338.34 335.52 343.84 372.1 0 6.13 55.54 279.93 2.98 186.48 93.75 1056.18 398.75 121.16 183.42 143.41 160.74 119.82 57.24 91.28 114.89 254.98 275.46 52.73 403.52 814.71 92.28 497.26 409.98 300.97 870.46 2.57 578.15 82.65 15.52 27.16 17.93 900.48 12.89 8.74 20.93 +ERCC-00073 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 154.45 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.03 23.13 7.76 6.29 0 0 0 1.93 0 0 1.92 0 0 0.74 0 1.06 0 0 0 0.82 12.12 0 0 0 0 0 0 0 0 0.95 1 5.64 0.84 0 0 0 0 0 0 0 0 0 0 0 0 0 1.98 0 5.52 0.62 0 0.75 0 0 +ERCC-00074 0 0 0 0 0 0 0 0 0 0 0 39.61 55.47 1496.38 11316.7 20493.28 15424.08 13873.51 25319.51 18341.25 15845.04 33064.42 16369.9 1852.72 18239.6 20415.4 9700.98 259.42 2167.61 6741.59 2805.8 6276.26 11792.22 1.76 0.76 0.92 204.73 0.66 4070.69 1901.75 104.1 1.63 128.59 377.66 2.16 31866.85 0 0 0 0 0 37696.59 29910.71 3989.6 6053.3 4840.43 19705 2701.08 32956.55 8000.51 41903.11 31811.29 42645.29 43540.5 9631.49 54566.68 47759.08 7087.24 34306.32 41036.5 56554.17 31596.96 22647.24 60986.15 89662.95 113693.98 186845.05 75826.63 17508.95 96225.52 9627.03 8660.14 20633.95 27678.63 39544.03 15435.3 9789.09 27513.41 10996.27 26621.92 138721.61 101838.73 41131.21 33495.99 16090.5 12880.61 69785.54 26543.32 105184.79 128381.14 158832.12 46306.05 37316.22 15510.38 27821.07 12698.37 16058.66 4242.21 68240.6 32041.82 94809.41 15285.07 65771.22 37692.51 63991.12 59641.32 13012.43 186.6 23765.69 33.36 3.5 1.62 91107.04 48863.22 34927.33 29681.16 107781.36 15903.44 56603.72 212264.44 218991.98 188665.49 140369.42 220883.06 105874.66 166710.77 155171.49 172611.01 231544.23 100677.41 116148.38 110192.29 112805.94 23775.33 80645.7 90157.4 81214.37 69640.78 166794.63 118173.79 162718.94 122724.9 163443.44 75809.3 115724.04 2082.24 83814.41 98421.44 30230.87 30103.27 101046.03 32743.21 96276.74 29354.98 56454.85 56278.42 135950.37 76214.36 104227.54 76728.22 118625.01 198659.52 56307.87 79610.9 69832.47 100739.69 +ERCC-00075 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 +ERCC-00076 0 0 0 0 0 0 0 0 0 0 0 0 3.13 3.94 102.68 337.65 151.88 102.88 228.39 147.93 116.53 257.86 111.94 17.8 36.78 249.16 77.57 0 0 3.76 324.38 2.41 0.89 0 0 0 0 0 0 0 0 0 0 0 0 487.07 0 0 0 0 0 88.17 388.89 89.15 3226.62 0 1862.96 454.85 0 4792.24 8.15 1242.24 0 0 62.34 0 157.76 0 133.86 23.61 3379.55 26.68 0 0 86.31 0 0 3398.13 0 3258.28 0 0 0.95 23.73 0 171.38 384.51 5.2 0 0 63.66 2.1 3800.89 24.13 2.58 0 0.41 12.58 6137.49 3203.72 4376.6 9.07 2673.35 21.03 4459.23 10.01 2465.46 1422.21 31.77 2785.96 4992.11 4905.69 16.48 2257.04 1206.03 128.15 4709.49 2.07 340.23 0 0 0 1338.85 1632.04 681.18 1016.37 1221.48 87.25 168.92 1589.16 2198.75 2391.86 2382.47 1422.46 982.93 1915.89 1945.4 1875.57 989.15 890.02 3357.48 1875.01 2616.68 780.15 458.04 1856.9 1922.19 3598.98 1047.08 1551.47 1454.89 1389.6 998.51 289.86 768.31 682.8 2132.68 2570.97 2011.72 4213.21 1490.7 891.65 1654.61 2731.72 1701.49 1064.26 101.32 479.67 1277.52 3878.12 4298.44 217.93 0.7 476.96 2407.86 3035.95 +ERCC-00077 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 72.32 0 0 31.72 3.11 0 0 7.92 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 41.55 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 23.95 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4.65 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 +ERCC-00078 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.34 19.62 0 60.13 6.72 0 46.62 119.78 17.27 0 0 46.99 8.68 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10.73 0 0 0 0 0 70.99 86.17 695.48 45.26 0 0 0 0 2361.67 0 0 0 0 0 0 0 0 0 0 702.97 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 88.24 0 0 0 0 0 0 1.38 0 0.22 0.75 2144.11 59.48 3.65 15.95 921.64 5524.61 0 0.99 124.52 0.81 229.71 60.28 169.75 0.21 571.38 3242.53 69.01 13.08 2.45 1.27 8.95 0 0 0 423.85 398.36 315.75 500.21 223.53 54.59 329.83 137.69 123.15 143.75 161.45 102.38 133.42 169.26 154.36 194.04 250.75 297.37 0 227.75 416.26 1204.57 143.36 856.68 188.91 750.79 160.35 163.8 89.51 136.97 138.59 233.1 138.21 907.31 244.46 409.68 426.41 741.97 389.01 331.1 144.52 334.32 420 246.55 5.62 180.79 66.22 7.09 19.75 16.62 0 276.68 7.58 1.75 +ERCC-00079 0 0 0 0 0 0 0 0 0 0 0 0 0 7.83 38.79 97.64 135.51 5.93 24.78 73.44 0 0 12.12 0 1.38 2.08 34.61 0.37 24.45 0 0 200.72 12.47 0 0 0 0 0 0 0 0 0 0 0 0 5.86 0 0 0 0 0 3641.12 0 11.09 0 0 47.54 0 0 20.23 56.77 0 3662.51 0 0 0 0 0 0 23.5 420.38 0 0 0 9.18 302.53 0.78 0 45.6 0 0 0 0 0.72 0 0 0 56.26 0 0 28.04 0 0 0 0 0 0 0 1.44 378.67 1701.48 392.52 0 1.96 2822.66 161.25 22.85 407.41 196.76 1.53 1.36 0.78 299.03 1.12 10.3 24.35 1395.84 0.68 5.05 0 0 0 286.39 351.63 122.13 138.34 231.54 25.36 44.97 229.38 266.3 308.18 292.78 219.38 229.44 317.97 252.75 269.8 636.89 422 408.8 527.5 163.52 30.4 281.82 1278.85 115.59 175.43 172.64 216.96 215.22 205.63 226.82 119.8 132.51 233.36 358.79 215.97 295.48 521.08 634.79 230.11 559.58 591.62 463.25 593.91 7.66 95.19 1153.39 12.69 34.14 1770.14 1032.02 464.49 498.77 479.14 +ERCC-00081 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.23 0 0 0 0 0 0 0 162.52 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.62 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 +ERCC-00083 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 +ERCC-00084 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 50.11 8.86 10.51 0 25.99 3.03 0 7.12 0 1.31 0 0.83 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 129.05 0 0 0 0 0 0 0 0 0 0 0 0 0 105.44 0 0 0 0 0 88.69 0 0 0 0 73.57 0.51 0 2287.35 1.08 0 3916.41 205.56 1653.85 0 0 0 0 0 0 0 51.31 0.68 0 0.47 0 0 0 2.69 0 0 0 0 0 103.63 505.56 0.39 0 0 673.28 0 0 0.4 0 0 0 0 0 0 0 0 0 0 81.15 0 0 0 157.85 289.96 108.47 256.84 191.66 389.97 162.99 54.81 61.16 73.2 75.51 46.63 43.03 69.94 62.87 75.34 643.85 150.36 48.84 771.86 490.53 676.66 140.69 0.79 0.37 11.12 63.81 37.57 58.82 49.66 49 72.88 69.23 142.24 2.62 97.37 102.46 84.94 96.43 88.44 353.75 383.51 2.52 401.08 0 0.38 37.93 594.92 15.08 2.41 0 393.85 1.68 0 +ERCC-00085 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 41.51 0 32.23 0 0.35 0 28.6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 53.15 0 0 0 0 0 0 0 0 0 0 0 0 0 40.7 588.15 122.3 11217.96 273.36 0 0 0 0 0 0 0 0 0 0 0 0.64 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5128.32 0 0 0.48 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51.84 61.51 0 12.63 46.8 309.8 45.29 7.84 17.3 12.07 13.37 10.08 4.57 8.77 11.57 12.75 0 330.21 0 0 1.87 0 0 0 0 354.26 9.23 11.51 2.89 8.37 10.59 14.01 8.3 174.3 127.04 0 112.46 0 0 3.5 0 7.19 0.9 12.6 0 0 10.88 1.9 2.88 0 0 0.45 0 0 +ERCC-00086 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 +ERCC-00092 0 0 0 0 0 0 0 0 0 0 0 0.14 1.98 51.1 101.16 511.32 181.18 190.38 438.61 168.66 157.54 296.07 171.34 0.37 206.73 318.36 84.35 0.71 0 251.35 348.33 105.78 162.61 0 0 0 0 0 0 0 0 0 0 14.35 0 237.73 0 0 0 0 0 10.09 258.56 37.3 943.38 117.78 4002.58 0 0 343.52 1763.68 0 679.17 5193.94 330.79 3771.18 6487.02 4087.99 212.8 91.29 2125.99 296.56 1574.72 2.9 127.98 5003.73 1264.41 786.3 0.33 1238.27 4.81 0 2134.68 3.42 1546.43 0 0.51 2309.74 0 33.27 3615.56 0.78 61.43 870.24 2.35 0.59 4110.08 6.12 3335.16 29.02 2246.01 5.01 962.14 3953.17 1763.05 6482.73 2828.43 2092.54 1357.24 818.13 3859.79 3298.62 1147.65 4875.18 1664.56 989.69 1011.4 3.68 164.95 0.13 0 0 1415.86 1986.3 1498.52 2431.24 1537.92 2736.53 1787.84 1188.28 1376.65 1562.6 1639.34 1023.04 908.52 1488.17 1479.14 1675.7 1556.95 2526.89 1977.32 2076.72 959.13 2358.73 1536.67 2702.74 1767.95 838.91 1169.89 1452.5 1334.24 1522.56 1404.65 1566.25 1365.2 2067.6 1026.37 1900.32 2039.52 2697.59 1697.62 2802.25 1837.68 1406.11 2929.93 1741.66 5675.8 861.56 435.76 3569.33 3240.67 74 1191.01 1393.55 557.84 898.42 +ERCC-00095 0 0 0 0 0 0 0 0 0 0 0 0 0 0 113.13 57.09 166.33 34.7 137.46 81.07 72.76 103.77 131.67 1.24 87.83 280.21 49.55 0.52 88.33 42.67 154.11 67.99 1.25 0 0 0 0 0 0 0 0 0 0 0 0 195.04 0 0 0 0 0 501.02 107.54 30.67 189.77 153.15 134.04 0 0 44.24 11.14 2200.21 0 0 12.26 1051.94 0 0 0 32.23 411.94 222.7 10.71 0 2.75 4.1 19.17 1.47 6.83 1070.29 0 0 13.11 202.54 0 364 4.84 0 0 0 62.57 1.76 0 1.29 1.22 0 29.5 0.99 458.14 136.05 271.26 0 0 0.91 265.08 6.37 136.87 0 191.68 0.53 1689.15 57.91 1273.16 107.01 105.52 200.61 6.22 0 146.92 0 0 0 16.52 19.59 3.92 1.58 38.36 10.89 47.48 467.23 1070.29 512.53 437.75 635.69 59.98 761.59 572.69 717.5 42.54 1324.52 103.25 113.34 10.24 54.53 895.18 4.85 95.93 259.83 254.7 261.55 217.29 204.93 236.09 34.58 265.78 7.81 23.92 135.85 30.02 25.5 63.88 4.36 399.21 72.8 95.52 166.01 0 7 60.67 0.76 1 4.74 557.4 12.54 1115.56 130.09 +ERCC-00096 0 0 0 0 0 0 0 0 0 0 0 60.08 59.93 1451.1 8416.16 19368.43 14226.49 18288.31 19976.02 13220.07 10788.19 20447 12739.93 1255.66 13580.89 16398.44 10568.1 299.39 1907.17 6531.51 2256.08 7453.68 12913.02 0.21 0.29 0 0.21 0 0 394.82 0.49 2.09 0.6 655.42 0.96 30490.51 0.21 0.23 0.24 0 0 185580.38 169733.07 15276.6 72845.62 46713.35 146024.02 23735.35 308563.86 63269.53 165309.33 163711.18 188664.93 193146.77 53716.52 208360.27 176231.56 353825.58 210583.86 210696.46 269652.99 181964.01 141182.01 200555.57 203485.51 95945.48 49295.11 67892.69 50796.32 65202.07 40796.52 51880.31 90718.07 98298.54 72336.96 104883.65 47840.02 98561.91 28603.84 113158.87 159105.33 105669.59 94976.82 100128.17 138153.87 139041.35 210493.52 66508.25 238800.02 146812.71 178252.53 54232.79 110804.19 104067.71 142432.34 140166.98 141082.75 49005.61 134515.42 92517.67 203715.57 131533.22 116489.48 103865.09 243120.96 151249.33 200369.74 438.01 20226.24 47.48 16.17 93.02 190833.36 136402.85 177810.34 202420.41 159424.59 156769.2 141029.78 76636.43 76302.81 102403.38 116590.61 69200.72 80306.17 85655.79 93868.17 98897.94 98363.69 81770.46 130514.54 156791.34 148191.85 121838.28 63709.97 125356.92 108550.68 90394.67 105479.96 125021.69 113574.09 130731.61 102759.25 122600.69 110897.43 125754.7 163198.11 113684.95 151663.9 215507.17 131531.14 156178.68 106765.98 124145.33 150110.55 152205.1 110871.95 69728.38 89990.84 168026.81 117571.06 118772.78 79400.47 86124.53 71529.3 83135.29 +ERCC-00097 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.15 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.78 0 0 0 0 0 0 0 0 0 0 +ERCC-00098 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 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61856.85 23.98 2069.27 0.45 6.91 0.1 21016.98 26774.97 19460.27 23291.7 13353.65 20060.36 18816.66 16578.13 22306.92 23367 24355.08 12852.93 15881.5 20091.78 18878.04 21852.2 11641.76 11298.94 15185.75 16815.01 37167.8 19964.16 10460.04 11911.11 13962.17 18692.45 18869.21 23214.56 20755.67 20766.68 15438.01 16402.51 17744.83 15132.28 14494.6 20976.84 15779.87 32485.04 18749.03 20842.07 19193.81 17609.48 19343.76 19758.86 23511.27 10600.77 12319.17 12151.85 17102.69 3520.88 15929.02 18969.58 11735.01 18653.82 +ERCC-00109 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.27 7.06 0 0 0 1.22 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.39 0 4.44 0 0 2.41 0 0 0 0 0 0 0 +ERCC-00111 0 0 0 0 0 0 0 0 0 0 0 0 0.14 18.39 105.34 308.07 382.56 517.46 227.85 739.35 168.08 847.36 136.61 24.86 254.9 521.8 232.03 0 0 132.43 134.06 314.4 735.82 0 0 0 0 0 0 0.18 0 0 0 1.15 0 816.01 0 0 0 0 0 7561.79 1204.91 973.69 1355.99 1653.41 4962.66 126.05 0 4.58 9003.72 2500.89 11437.18 2981.58 395.15 10225.06 1883.51 238.11 594.66 212.15 6858.01 3759.39 53.77 7098.5 16005.76 8077.89 15.34 2086 12.84 4410.46 60.97 3650.15 377.19 7.97 2644.91 3005.98 2295.65 3520.36 3314.45 2.36 725.18 416.03 2.67 15561.18 2003.12 4.84 1781.13 33.15 10027.34 5557.75 5892.78 8766.53 5668.8 1352.16 6753.75 7575.68 6598.12 616.19 4457.63 4467.63 477.02 5121.01 1239.09 2126.18 8408.95 7804.3 13318.21 11.1 544.95 0.15 0.22 0 4564.72 4443.1 4563.56 3652.71 3923.12 4478.58 4455.57 2022.56 2811.52 2414.09 2569.09 1944.42 3440.11 2960.87 2716.48 2937.03 2334.6 4614.09 2154.54 2736.54 4729.58 3403.04 3295.34 3885.67 3856.9 2507.56 3574.02 4248.74 3554.98 3124.87 3275.55 3882.79 3499.47 3162.57 3447.69 4612.21 3737.77 3569.72 3567.85 4819.84 5454.25 3077.75 4422.55 5054.5 4683.55 1542.55 7051.2 1464.83 4455.06 655.87 4801.17 2942.5 6025.28 4677.92 +ERCC-00112 0 0 0 0 0 0 0 0 0 0 0 0 0 0.57 6.42 157.88 79.73 157.2 110.92 24.26 148.38 248.84 152.98 0 14.37 242.19 62.81 0 0 24.48 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 202.4 0 0 0 0 0 0 0 42.08 2398.04 0 21.94 13.35 0 1245.59 12677.21 75.44 2769.05 17980.9 175.82 0 0 807.05 6701.18 22.53 550 0 15160.57 13571.88 2.75 891.34 11.92 1.48 4.61 424.13 0.68 0.83 2.21 359.89 12.32 0 0 0 0.53 9603.52 6.3 14202.79 0.22 0.62 3.09 1.46 6513.91 1.27 2859.58 1083.85 514.12 471.01 4654.89 45.83 17.38 12.57 2418.7 7.2 2576.77 0.89 21.64 0.9 187.57 808.38 15104.99 715.66 4.15 1.55 139.97 0 0 0 1079.55 999.21 887.68 1001.14 580.83 1803.08 1215.43 394.33 461.9 408.84 378.71 287.82 301.16 515.65 466.66 531.22 828.7 574.86 5.62 225.62 1158.43 2489.4 938.06 133.4 597.52 623.32 500.42 431.21 444.48 360.92 493.21 620.24 537.16 1416.67 396.83 1143.37 803.88 1052.56 1185.48 1014.55 822.8 1027.43 1730.47 255.48 5.93 311.48 1174.3 12.79 148.13 15.77 1384.4 1723.2 1591.21 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43213.26 42824.25 38839.85 32872.19 62032.09 68375.65 62143.31 44639.71 27523.94 49742.94 38082.15 34487.53 39692.82 51852.42 46053.03 49891.89 41020.52 30265.05 42293.64 46973.52 54282.02 46540.43 57623.05 74041.66 52726.71 40741.97 36813.2 52269.99 49028.01 45434.6 30139.81 28698.81 59633.49 67163.87 47474.6 54560.47 24421.37 33198.41 26206.7 35602.11 +ERCC-00116 0 0 0 0 0 0 0 0 0 0 0 0 0 36.24 88.79 100.66 261.26 164.52 76.14 42.97 56.64 211.24 113.64 9.16 148.15 211.76 199.37 0.09 11.53 2.55 0 19.48 388.65 0 0 0 0 0 0 0 0 0 14.89 0 0 394.75 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11.43 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6.74 244.7 0.06 0 0 0 3.67 0 34.68 0 135.11 0 0.22 0 0 0.88 0 0.61 0.17 0.3 0 0 0 0 0.37 0.22 0 0 0 0 0 1.68 2.46 1.58 0.87 0.9 10.88 2.77 0.22 0 0 0 0 0 0.25 0 0 0 0 0 0 0.42 0.14 0.84 0.13 0 0 0.18 0 +ERCC-00117 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 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199575.54 205821.79 155079.57 201028.42 121385.94 279097.9 85725.57 54422.76 131082.31 98953.6 117160.62 87578.9 50549.59 26166.03 60007.4 37983.48 59382.37 91727.87 62945.61 43221.44 72392.4 55826.88 101106.13 54417.95 57728.26 119636.95 88444.96 58193.58 150657.35 48733.6 77145.1 160443.57 53791.67 129364.36 110128.15 120291.24 38148.91 90840.67 76622.72 80656.81 83050.09 90961.53 34048.12 101027.15 64887.54 149797.11 87256.14 72513.11 66103.17 169296.62 91573.48 128205.89 466.33 22746.9 60.55 3.1 44.99 126802.49 92556.42 117696.29 122486.35 112996.86 106630.54 101953.88 63595.7 62889.31 77161.04 92289.85 60377.03 64482.7 73613.98 77630.8 76627.07 77306.05 66310.77 107575.4 103133.39 100093.3 89422.23 57268.44 86713.9 79341.07 80263.86 89025.36 95324.1 88518.55 109559.31 90767.55 95321.66 98076.31 59520.23 108059.38 83733.67 114664.64 142431.37 95128.67 117802.25 85476.62 94676.47 123640.4 116465.72 86853 58031.96 92481.41 112062.61 88625.09 124451.34 58274.03 76503.18 63795.5 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36.68 76.81 63.32 77.11 349.42 93.82 2.67 0.97 209.14 48.84 3.09 4.52 0.89 2.24 8.53 19.31 18.55 13.26 8.19 13.31 15.77 1.22 95.59 3.89 50.91 165.97 4.8 8.13 37.72 9.35 93.28 19.13 1.67 0 56.51 3.57 14.98 2.94 207.75 1.79 292.09 0 +ERCC-00164 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.5 0 0 0 1.84 0 0 0 0 0 0 0 0 0 0 0 10.71 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.49 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 +ERCC-00165 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 228.66 0 0 10.93 122.26 35.25 269.48 27.61 0 1.22 15.55 22.35 0 0 185.33 84.18 0 23.57 0 0 0 0 0 0 0 0 0 0 0 0 128.67 0 0 0 0 0 2013.47 50.92 21.99 0 52.43 0 0 0 0 0 0 0 0 0 8895.38 13.01 281.71 0 0 1256.26 168.84 0 0 0 0 0 0 0 43.02 0 0 0 0 134.77 2826.47 0 0 0 1.12 0 0 0.32 0 221.69 0.82 0.26 0 30 0 0 0 3869.67 23.64 2550.15 15.31 7880.81 1889.2 0 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778.22 2865.33 2268.88 3630.68 18.58 1047.05 0 686.65 4071.35 987.7 10078.47 0 0 0 2246.99 0 1165.43 3028.78 1131.87 2664.91 4124.58 1876.75 1493.8 451.01 10207.2 0 380.37 3701.82 6195.66 320.02 6257.34 395.96 10.19 55.74 0 0 32.88 63.11 247.59 19.48 79.09 41.71 170.73 132.19 0 0 0 0 0 0 0 0 0 212.46 107.21 0 4.72 109.35 880.65 144.7 472.09 284.83 187 0 39.57 0 0 0 0 5.34 46.39 291.35 303.14 184.39 30.46 0 533.13 186.08 95.51 500.04 280.19 195.75 559.96 66.45 0 0 0 461.88 646.37 187.92 261.42 +LSR1 0.29 76.59 38.46 0.31 2.08 0 0 0 0 0 0 2.91 1.1 0.82 0 0 0 23.02 0.37 0 0 0 31.44 7.67 5.36 0 21.84 1.68 0 0 0 0 0 0 0 0 0 0 0 0 0 4.76 0 0 0 6.34 0 0.21 0.22 0 345.74 0 0 0 257.04 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18.38 0 0 0 0 0 0.18 0 0 0 0 0 0 0 0 0 0 0 0 65.77 0 0.35 0.67 0 0 0 0 3.94 6.73 0 0 0 0 3.57 0 0 0 0 1.24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.43 70.6 0 0 0 0 0 0 0.99 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 +NME1 0 0 0 0 0 0 0 1.4 0 0 0 0 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1178.19 1613.36 2322.68 2925.3 892.21 2144.28 1699.82 894.77 1531.37 265.86 1391.86 3.34 2145.64 3541.21 2172.24 1345 2380.51 1705.77 2573 1904.33 979.88 1696.15 3690.41 3065.73 1502.05 2188.05 2926.18 2339.37 1883.77 3711.05 7439.78 5796.99 +RDN18-2 75.75 0 0 0 0 265.37 0 63.31 20.71 0 0 7944.79 4859.13 289.55 201.78 481.23 0 326.95 664.25 45.46 0 482.41 144.71 0 103.73 6.69 141.77 1.84 0 0 218.76 48.72 10.25 2179.85 6680.61 4581.11 687.54 3169.57 876.37 2224.92 3107.55 2609.19 649.47 994.78 4304.62 1391.09 2383.34 507.43 839.22 9165.29 2318.4 339.73 500.85 2773.16 8416.83 10063.08 2283.7 8655.66 723.95 17721.66 114.46 1061.43 816.37 1952.07 12774.86 2951.51 1383.14 830.86 202.37 361.38 24.81 49.78 6351.29 0 0 0 43.97 2365.76 6346.24 3854.97 7929.6 7522.1 10193.25 5892.67 5475.08 2368.63 9872.04 6078.43 14381.67 1661.31 361.89 2215.6 9581.78 2515.42 4130.04 13791.13 7.99 4368.16 684.56 604.57 406.95 5365.03 191.96 4489.11 2284.73 251.64 2215.07 1866.3 1114.83 298.05 127.79 1744.74 20422.08 1382.19 390.59 1719.41 3298.93 385.38 171.79 35.11 51.71 17.78 1446.94 3536.65 2807.74 4634.3 3329.91 682.34 3459.09 231.9 161.87 393.41 60.65 47.34 11.21 121.9 130.38 179.01 2421.68 2992.99 1651.12 1636.75 3933.21 2169.63 1178.19 1613.36 2322.68 2925.3 892.21 2144.28 1699.82 894.77 1531.37 265.86 1391.86 3.34 2145.64 3541.21 2172.24 1345 2380.51 1705.77 2573 1904.33 979.88 1696.15 3690.41 3065.73 1502.05 2188.05 2926.18 2339.37 1883.77 3711.05 7439.78 5796.99 +RDN25-1 394.64 0.03 965.5 0 849.71 0 127.29 0 1921.05 126.12 901.63 27544.34 20318.34 5753.13 13474.89 8748.95 3552.96 6598.07 5968.15 16748.32 6316.15 6764.86 5271.35 2419.89 6059.03 7417.76 3526.02 5784.04 607.26 994.35 697.54 851.89 1445.94 2781.42 16871.33 3560.66 1870.7 3425.22 2641.04 1777.23 2692.15 9493.15 10938.12 26290.86 11818.76 9506.49 5447.93 6132.68 1426.51 19012.44 26022.16 724.04 2977.31 35515.93 24168.29 13782.8 14828.9 31884.77 4013.58 4865.78 176.36 9937.57 1756.57 1030.7 6079.19 369.89 297.77 985.82 592.37 476.54 71.2 159.09 361.34 133.32 106.25 539.82 1871.85 5992.05 12777.08 3351.48 2486.8 3467.3 9086.3 14448.63 4313.92 6413.46 5624.57 2748.68 6525.05 2332.7 1145.71 428.07 3305.68 716.72 3827.7 2464.51 64.02 4248.28 944.52 1675.37 739.59 22223.29 9223.15 14138.12 8996.26 8651.92 11553.45 25067.62 9408.84 10224.67 916.02 15088.59 11063.23 12594.11 1181.69 10349.85 6320.92 1509.31 5177.28 270.92 293.55 138.1 1677.71 1375.35 1223.48 1614.09 1755.43 447.84 1768.33 356.69 283.3 848.41 319.34 199.03 84.63 409.87 318.48 340.73 924.8 2261.38 1721.15 2091.59 1605.75 1843.36 1431 1235.11 1835.73 1331.41 1070.19 1767.95 1247.06 951.15 1003.57 668.19 1148.97 146.59 1630.53 2032.29 1567.79 916.36 1529.6 1792.02 1191.85 1236.88 1914.5 1593.24 2478.03 1576.55 1397.64 2236.27 1681.68 1005 1952.29 1905.83 2250.74 2674.92 +RDN25-2 394.64 0.03 965.5 0 849.71 0 127.29 0 1921.05 126.12 901.63 27544.34 20318.34 5753.13 13474.89 8748.95 3552.96 6598.07 5968.15 16748.32 6316.15 6764.86 5271.35 2419.89 6059.03 7417.76 3526.02 5784.04 607.26 994.35 697.54 851.89 1445.94 2781.42 16871.33 3560.66 1870.7 3425.22 2641.04 1777.23 2692.15 9493.15 10938.12 26290.86 11818.76 9506.49 5447.93 6132.68 1426.51 19012.44 26022.16 724.04 2977.31 35515.93 24168.29 13782.8 14828.9 31884.77 4013.58 4865.78 176.36 9937.57 1756.57 1030.7 6079.19 369.89 297.77 985.82 592.37 476.54 71.2 159.09 361.34 133.32 106.25 539.82 1871.85 5992.05 12777.08 3351.48 2486.8 3467.3 9086.3 14448.63 4313.92 6413.46 5624.57 2748.68 6525.05 2332.7 1145.71 428.07 3305.68 716.72 3827.7 2464.51 64.02 4248.28 944.52 1675.37 739.59 21057.93 9223.15 14138.12 8996.26 8651.92 11553.45 25067.62 9408.84 10224.67 916.02 15088.59 11063.23 12594.11 1181.69 10349.85 6320.92 1509.31 5177.28 270.92 293.55 138.1 1677.71 1375.35 1223.48 1614.09 1755.43 447.84 1768.33 356.69 283.3 848.41 319.34 199.03 84.63 409.87 318.48 340.73 924.8 2261.38 1721.15 2091.59 1605.75 1843.36 1431 1235.11 1705.05 1331.41 1070.19 1767.95 1247.06 951.15 1003.57 668.19 1148.97 146.59 1630.53 2032.29 1567.79 916.36 1529.6 1792.02 1191.85 1236.88 1914.5 1593.24 2478.03 1576.55 1397.64 2236.27 1681.68 1005 1952.29 1905.83 2250.74 2674.92 +RDN37-1 340.63 2257.5 1209.86 909.67 493.22 106.96 93.89 98.01 111.55 64.81 920.18 463.17 466.99 719.95 924.48 437.78 437.47 265.13 699.21 585.24 311.1 186.19 403.53 218.83 105.19 887.95 428.12 315.04 99.96 127.12 19.02 164.57 344.94 403.39 1391.05 71.62 192.59 27.36 24.16 272.83 335.53 895.24 2157.58 1418.48 1176.67 587.42 522.78 936.1 256.16 2387.5 1526.1 9.47 20.33 502.25 843.96 286.06 878.4 609.17 0 559.22 0.35 20.45 0 9.07 31.62 5.49 2.41 51.31 0.11 5.8 2.3 9.71 24.82 0.57 0.95 1.56 16.34 990.67 254.42 476.64 548.93 1061.49 2936.59 1199.92 1280.71 1222.79 1523.93 926.73 1463.76 38.47 350.59 8.41 317.91 1998.96 393.08 1926.26 2.58 453.39 2.92 1079.27 27.92 718.63 1074.45 867.49 1002.63 1165.69 767.08 1229.26 294.32 4497.5 17.67 321.51 1645.59 2123.4 75.16 1815.08 116.06 50.89 131.85 11.61 46.13 35.36 52.49 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0 0.31 0 0 0.44 0 0 0 0 0 0 0 0 2.59 0.21 2.75 0.86 0 0 0 0 3.42 0.26 3.26 0 0.09 0 43.18 0.71 17.62 0 0 0 0 0 0 +YKL106C-A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 50.74 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 +YKL106W 28.27 6.24 0 7.24 18.94 0 459 0.15 2.5 0 0 3.33 1.2 24.97 0 0.21 0 9 0 0 7.4 19.73 0.9 13.65 31.85 21.06 17.51 5.38 0 0 2.33 67.27 0 0.99 29.45 0 1.74 0 0 0 35.71 27.03 3.5 5.93 0 4.84 0 117.36 0 17.06 0 0 0 0 113.37 0 0 0 0 128.18 3.14 0 0 0 3.39 0 0 0 0 9.14 0 0 0 0 0 0 0 0 0 0 0.55 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.22 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 53.3 32.79 68.18 105.05 0 0 0.38 0 0 0 0 0.26 0 0.47 0 0 0 0.32 0 0 0 0 0 0 0 0 0 20.07 0 0 8.19 0 0.54 0 0 0 0.28 0 0 0 1.93 0 0 0 0 0 16.68 0 0 0 0 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17.19 36.69 56.06 19.95 36.54 0 0 21.82 0.71 0 0 0 0.24 0 0 21.63 24.79 0 7.72 0.26 0 201.18 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.45 0 0 0 0 0 0 0 55.99 28.77 72.23 123.4 0 0 2.91 0 0 0 0.45 12.65 1.05 0 1.5 0 0.44 0 0.4 1.07 0.58 0 0 0 1.77 0 0.43 0 17.3 0 0 0 0.84 0 0 0.54 1.73 0.9 18.64 0.93 1.2 0 0.5 0.43 3.62 0 1.55 8.49 0 0 0 3.13 1.98 0 0 0 0 0 0 +YKL112W 1.33 0.05 13.01 32.99 0 0 0.03 0 0.07 0 0 0.38 4.07 8.7 22.45 7.77 62.31 2.21 0 11.21 37.5 109.02 32.81 32.73 16.68 0.08 26.64 8.75 0 0.51 23.01 0 0 40.41 0 0.22 0 0 0 18.38 1.92 29.31 2.25 8.48 2.26 10.01 0 293.73 0.22 0.08 0.09 0 0 7.4 5.95 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18.19 0 0 0 0 0 0 0 0 0 4.14 0 0 47.86 0 1.82 0 0 32.75 19.58 14.68 65.55 0 0 0.22 0.61 0 0 1.78 0.45 0 0 0 0 0 0.18 0 0 0 0 0 0 0 0.2 0 0 0 22.97 0 0.25 0 0 0 0.2 0.32 0.33 23.33 0 0 0 0 0.16 0 0 0.37 2.37 0 0 0 0.38 0 0 0 0 0 0 0 +YKL113C 5.96 0.43 2.27 84.05 4.28 0 0.12 0.03 46.67 0.19 0.07 2.86 18.59 118.93 8.35 89.57 92.86 154.66 0.19 41.33 124.62 23.06 359.8 13.28 33.47 11.34 19.32 90.75 27.7 339.09 10.85 0 0 0 0 0 10.78 0 0 0 0 40.79 0.14 0 151.38 5.72 0 49.39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.31 0 0 0 0 0 0 0 0 0 0 0 0 47.3 18.97 47.25 5.44 33.35 0.43 6.86 0 0 0 0 16.83 1.23 0.56 0 1.11 0 3.09 1.25 0.56 0.46 0 0 0 0 0 0.34 1.03 0 0 0 2.1 2.63 0 0 0.42 10.14 1.05 1.28 0 0 0 12.72 0 0.47 0 4.41 0 0 0 0.32 7.28 0.26 0 0 0 0 0 0 +YKL114C 115.31 19.19 75.58 141.3 68 217.84 2.45 225.75 163.84 124.16 0.04 58.71 68.85 122.1 58.65 253.51 119.43 124.47 16.19 18.65 5.89 252.72 122.76 217.22 41.82 0 28.06 104.9 0.21 73.87 0.3 227.04 0 47.03 694.85 0 0 0 0 102.27 0 39.41 0 0 1.21 0 18.13 0 0.24 0 0 0 0 70.81 0 64.37 136.45 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.34 0 0 0 0 0 0 0 32.65 0 0 37.27 0 0 0 0 116.02 56.35 61.7 0 75.16 0 18.72 2.04 2.25 2.34 0.73 14.68 6.02 8.29 2.48 4.35 8.27 13.81 6.56 7.92 2.4 0 0 0 0 0 142.37 72.43 0 57.82 231.43 4.95 3.45 1.04 2.14 5.32 5.68 12.14 60.52 0 38.57 14.96 0 3.14 0 31.17 0 1.57 32.38 0 316.9 2.55 0 0 0 0 0 0.37 0 +YKL116C 21.42 7.39 0 0 34.1 0 0.04 0.02 0 17.62 1.37 0.66 3.11 6.9 26.78 17.42 11.66 16.02 0 27.52 0.13 0.14 242.97 98.01 22.07 163.44 27.33 43.6 0 153.17 0 0 0 93.99 5.61 0 0 0 0 0 3.97 0.63 0.39 0 10.04 124.35 0 0 0.16 387.48 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 35.06 0 0 0 0 0 0 15.95 0 0 0 1.8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 44.98 104.42 53.12 0.07 61.78 1.2 1.61 0 0 0.26 0 9.28 0 0.4 0 0 0 0.81 0.22 0.2 0 0 0 0 1.45 0 6.04 0 0 29.43 22.99 0.74 0 0.69 0 0 0.94 0.49 0 0 1.65 0 0 0 2.6 3.78 0.56 19.85 0 0.81 0 1.68 0 0 0.35 0 0 0 0.25 +YKL117W 698.08 510.62 518.47 674.01 723.81 63.68 91.6 1017.99 885.44 286.64 773.15 95.08 72.07 274.45 429.18 462.68 437.93 330.84 208.45 372.18 476.9 813.91 267.47 301.33 428.51 544 390.08 797.55 1383.35 449.08 133.6 1131.45 1863.58 110.62 27.12 78.11 0 0.45 0 140.25 102.52 407.07 123.05 184.99 180.03 280.17 509.67 4.69 429.36 0 15.5 0 0 109.21 107.15 27.62 0 0 0 0 0 0 0 0 26.18 0 0 0 0 0 0 310.03 0 0 0 0 0 115.32 84.23 148.76 0 15.92 185.21 94.42 90.94 75.69 0 443.56 105.57 8.38 0 0 0.98 0 0 138.64 0 0 0 0.58 0 2.05 0 0 5.65 0 22.48 1.41 0 1.88 1.34 2.68 0 4.42 0 98.75 0 554.69 448.5 456.02 462.25 442.9 35.24 215.19 56.64 28.14 15.88 111.09 68.08 118.94 95.19 75.3 77.54 88.27 85.92 122.41 100.69 124.75 160 164.32 0 42.65 30.56 31.36 212.04 49.48 84.42 192.65 88.28 52.76 55.04 57.62 71.17 56.79 78.25 52.14 160.39 134.5 168.31 25.98 66.82 83.23 61.01 134.34 24.22 63.25 164.53 0.69 56.28 1.09 30.08 0.55 203.22 110.17 0 83.54 +YKL119C 75.38 166.93 142.03 79.43 191.05 0 0.52 72.55 2.05 0 0 20.35 24.89 112.42 104.35 54.71 33.9 54.17 48.11 47.12 0.86 12.29 29.97 15.85 111.13 79.97 47.83 68.41 0 21.87 41.07 12.58 0 5.08 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0 0.35 0 0 0 0.53 0.79 0 0.38 34.59 6.59 102.96 1.62 0.51 1.15 0.16 1.13 4.5 0.93 0 0.28 0 0.67 0 1 0 0.23 0.75 0 0 0 66.73 2.73 0 0 0 0 0 0 0.95 +YNR048W 17.05 8.91 51.56 0.92 90.51 0 0.77 79.03 0.94 0 0 4.08 16.4 34.06 62.62 0 6.29 52.91 0 26.1 85.03 44.2 74.41 135.63 32.16 98.15 34.59 62.35 0 0 0 0 0 0 0 0 0 0 0 0 0 33.1 9.69 2.78 0 0 0 0 0 0 0 0 0 0 0 0 0 241.8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51.24 25.84 55.09 153.24 99.75 3.73 12.39 1.25 0.52 3.22 0 20.55 0.4 0 0 0.27 0 4.86 1.51 0.27 0.88 0 0 34.8 6.69 0.4 0 0 0 0 0 1.01 1.27 0 0 2.45 2.94 2.37 0 0 0 0 0 0 4.53 0 0 19.62 0 0 0 2.34 0 0 0 0 0 0 0 +YNR049C 84.24 24.55 278.52 0.76 55.52 23.71 1391.59 110.16 24.55 0 0.02 2.17 10.39 61.11 20.2 0 11.43 115.49 45.89 1.98 25.34 55.58 2.84 6.08 33.11 41.61 49.91 80.33 108.38 89.05 155.36 0 0 0 0 0 0 1746.9 0.41 0 0 18.9 0 0 0.52 252.4 5.25 0 0.94 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 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0 0 8.33 0 3.03 0.21 0 0.77 0 0 +YNR053C 41 20.54 0.44 0.02 3.11 0 0.18 0.02 0.31 0.14 173.06 18.91 20.59 3.52 0 12.54 14.71 0 0 1.64 0 0 5.71 0 1.16 0 0 32.42 105.71 22.33 0.53 17.94 0 5.15 0.43 0 1.58 0 0 0 0 97.83 81.46 30.82 51.71 164.8 82.82 0 0 1.7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4.73 1.95 0 36.02 0 0.32 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4.84 7.54 0 0 0 0 0.45 28.81 0 0 0 0 0.32 0 0.79 0.32 0 0 0.67 0 0 0 0 0 0 0 0 0 0.6 0 0 0 0 0 0 0 +YNR054C 49.44 42.38 18.8 0.13 5.98 0 1.32 238.17 1.4 0 0.04 31.6 5.09 13.4 0 0 0 0 0 0 0 0 0.88 1.85 0.62 0 0 63.98 0 0 0 173.21 0 0 0 1382.77 4.32 0 0 0.19 0 25.27 30.92 0 67.44 0 200.42 0 466.96 0 0 0 0 32.79 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.77 443.72 0.53 0.66 0 0 0.22 0 0 0 0 0 0 0 9.53 1.98 0.49 0 0 0 4.6 0 0 0 5.73 0.81 0.52 1.42 4.46 0 0 3.91 0.4 1.41 1.15 0 0 0 2.19 0 0 0 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ID:Bowtie VN:1.0.0 CL:"bowtie ./tests/sacCer3 ./tests/renilla_basic.fastq --threads 2 -m 21 --sam -S ./tests/renilla_basic.sam" -candidate-29-28 4 * 0 0 * * 0 0 ACGAAGCTCTTGATGTACTT IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-28-27 4 * 0 0 * * 0 0 CACGAAGCTCTTGATGTACT IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-30-29 4 * 0 0 * * 0 0 CGAAGCTCTTGATGTACTTA IIIIIIIIIIIIIIIIIIII XM:i:0 -candidate-31-30 4 * 0 0 * * 0 0 GAAGCTCTTGATGTACTTAC IIIIIIIIIIIIIIIIIIII XM:i:0 +candidate-29-28 4 * 0 0 * * 0 0 ACGAAGCTCTTGATGTACTT IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-32-31 4 * 0 0 * * 0 0 AAGCTCTTGATGTACTTACC IIIIIIIIIIIIIIIIIIII XM:i:0 +candidate-31-30 4 * 0 0 * * 0 0 GAAGCTCTTGATGTACTTAC IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-33-32 4 * 0 0 * * 0 0 AGCTCTTGATGTACTTACCC IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-34-33 4 * 0 0 * * 0 0 GCTCTTGATGTACTTACCCA IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-35-34 4 * 0 0 * * 0 0 CTCTTGATGTACTTACCCAT IIIIIIIIIIIIIIIIIIII XM:i:0 -candidate-37-36 4 * 0 0 * * 0 0 CTTGATGTACTTACCCATTT IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-36-35 4 * 0 0 * * 0 0 TCTTGATGTACTTACCCATT IIIIIIIIIIIIIIIIIIII XM:i:0 -candidate-38-37 4 * 0 0 * * 0 0 TTGATGTACTTACCCATTTC IIIIIIIIIIIIIIIIIIII XM:i:0 +candidate-37-36 4 * 0 0 * * 0 0 CTTGATGTACTTACCCATTT IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-39-38 4 * 0 0 * * 0 0 TGATGTACTTACCCATTTCA IIIIIIIIIIIIIIIIIIII XM:i:0 +candidate-38-37 4 * 0 0 * * 0 0 TTGATGTACTTACCCATTTC IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-40-39 4 * 0 0 * * 0 0 GATGTACTTACCCATTTCAT IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-41-40 4 * 0 0 * * 0 0 ATGTACTTACCCATTTCATC IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-42-41 4 * 0 0 * * 0 0 TGTACTTACCCATTTCATCT IIIIIIIIIIIIIIIIIIII XM:i:0 @@ -36,8 +36,8 @@ candidate-43-42 4 * 0 0 * * 0 0 GTACTTACCCATTTCATCTG IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-44-43 4 * 0 0 * * 0 0 TACTTACCCATTTCATCTGG IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-45-44 0 chrII 461264 255 20M * 0 0 ACTTACCCATTTCATCTGGA IIIIIIIIIIIIIIIIIIII XA:i:2 MD:Z:5A10A3 NM:i:2 candidate-46-45 4 * 0 0 * * 0 0 CTTACCCATTTCATCTGGAG IIIIIIIIIIIIIIIIIIII XM:i:0 -candidate-108-107 4 * 0 0 * * 0 0 CGGTGTTAGGGAACTTCTTA IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-47-46 4 * 0 0 * * 0 0 TTACCCATTTCATCTGGAGC IIIIIIIIIIIIIIIIIIII XM:i:0 +candidate-108-107 4 * 0 0 * * 0 0 CGGTGTTAGGGAACTTCTTA IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-109-108 4 * 0 0 * * 0 0 GGTGTTAGGGAACTTCTTAG IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-110-109 4 * 0 0 * * 0 0 GTGTTAGGGAACTTCTTAGC IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-111-110 4 * 0 0 * * 0 0 TGTTAGGGAACTTCTTAGCT IIIIIIIIIIIIIIIIIIII XM:i:0 @@ -50,16 +50,16 @@ candidate-136-135 4 * 0 0 * * 0 0 GACAATAGCGTTGGAAAAGA IIIIIIIIIIIIIIIIIIII XM:i candidate-137-136 4 * 0 0 * * 0 0 ACAATAGCGTTGGAAAAGAA IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-138-137 16 chrXIII 326231 255 20M * 0 0 GTTCTTTTCCAACGCTATTG IIIIIIIIIIIIIIIIIIII XA:i:2 MD:Z:15C1A2 NM:i:2 candidate-139-138 4 * 0 0 * * 0 0 AATAGCGTTGGAAAAGAACC IIIIIIIIIIIIIIIIIIII XM:i:0 -candidate-166-165 4 * 0 0 * * 0 0 GGACTCGATGAACATCTTAG IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-165-164 4 * 0 0 * * 0 0 CGGACTCGATGAACATCTTA IIIIIIIIIIIIIIIIIIII XM:i:0 +candidate-166-165 4 * 0 0 * * 0 0 GGACTCGATGAACATCTTAG IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-167-166 4 * 0 0 * * 0 0 GACTCGATGAACATCTTAGG IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-168-167 4 * 0 0 * * 0 0 ACTCGATGAACATCTTAGGC IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-169-168 4 * 0 0 * * 0 0 CTCGATGAACATCTTAGGCA IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-170-169 4 * 0 0 * * 0 0 TCGATGAACATCTTAGGCAG IIIIIIIIIIIIIIIIIIII XM:i:0 -candidate-172-171 4 * 0 0 * * 0 0 GATGAACATCTTAGGCAGAT IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-171-170 4 * 0 0 * * 0 0 CGATGAACATCTTAGGCAGA IIIIIIIIIIIIIIIIIIII XM:i:0 -candidate-174-173 4 * 0 0 * * 0 0 TGAACATCTTAGGCAGATCG IIIIIIIIIIIIIIIIIIII XM:i:0 +candidate-172-171 4 * 0 0 * * 0 0 GATGAACATCTTAGGCAGAT IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-173-172 4 * 0 0 * * 0 0 ATGAACATCTTAGGCAGATC IIIIIIIIIIIIIIIIIIII XM:i:0 +candidate-174-173 4 * 0 0 * * 0 0 TGAACATCTTAGGCAGATCG IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-175-174 4 * 0 0 * * 0 0 GAACATCTTAGGCAGATCGT IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-176-175 4 * 0 0 * * 0 0 AACATCTTAGGCAGATCGTC IIIIIIIIIIIIIIIIIIII XM:i:0 candidate-385-384 4 * 0 0 * * 0 0 GGTCTCGACGAAGAAGTTAT IIIIIIIIIIIIIIIIIIII XM:i:0 diff --git a/tests/test_alignment.py b/tests/test_alignment.py index ff294cb..0361a0e 100644 --- a/tests/test_alignment.py +++ b/tests/test_alignment.py @@ -5,7 +5,6 @@ from eFISHent.alignment import AlignProbeCandidates from eFISHent.alignment import PrepareAnnotationFile -from eFISHent.constants import COUNTS_COLUMNS def test_prepare_annotations(): @@ -21,26 +20,23 @@ def test_prepare_annotations(): @pytest.fixture -def task_align(): +def task_align() -> AlignProbeCandidates: task = AlignProbeCandidates() task.fname_genome = "./tests/sacCer3" return task -def test_read_count_table(task_align): - fname_counts = "./tests/count_table1.tsv" - df = task_align.read_count_table(fname_counts) - for col in COUNTS_COLUMNS: +def test_read_count_table(task_align: AlignProbeCandidates): + fname_count = "./tests/count_table1.tsv" + task_align.fname_count = fname_count + df = task_align.count_table + for col in ["gene_id", "count"]: assert col in df.columns - - # Bad count table with clean_id instead of gene_id - fname_bad = "./tests/count_table2.csv" - with pytest.raises(ValueError): - task_align.read_count_table(fname_bad) + assert df["count"].dtype == float @pytest.mark.parametrize("gene,endogenous", [("renilla", False), ("aad4", True)]) -def test_align_probes(task_align, gene, endogenous): +def test_align_probes(task_align: AlignProbeCandidates, gene, endogenous): fname_sam = f"./tests/{gene}_basic.sam" fname_fastq = f"./tests/{gene}_basic.fastq" task_align.fname_fasta = f"./tests/{gene}_basic.fasta" @@ -64,7 +60,7 @@ def get_number_of_alignments(fname): @pytest.mark.parametrize("gene", ["renilla", "aad4"]) -def test_filter_unique_probes(task_align, gene): +def test_filter_unique_probes(task_align: AlignProbeCandidates, gene): fname_sam = f"./tests/{gene}_basic.sam" task_align.fname_gene = gene task_align.fname_sam = fname_sam @@ -76,25 +72,41 @@ def test_filter_unique_probes(task_align, gene): assert len(pd.merge(df_endo, df_exo, how="inner", on="qname")) == 0 -def test_exclude_gene_of_interest(task_align): +def test_exclude_gene_of_interest(task_align: AlignProbeCandidates): df = pd.read_csv("./tests/count_table2.csv") # Using ensembl ID ensembl = "ENSG00000281100" - df = task_align.exclude_gene_of_interest( + out = task_align.exclude_gene_of_interest( df, ensembl_id=ensembl, fname_full_gene="", threads=2 ) - assert ensembl not in df["clean_id"] + assert ensembl not in out["gene_id"] # Using blast - # Create count_table with gtf merge! - # aad4_gene_id = "YDL243C" - # df = task_align.filter_gene_of_interest( - # df, ensembl_id="", fname_full_gene="./tests/aad4.fasta", threads=2 - # ) - # assert aad4_gene_id not in df["clean_id"] + aad4_gene_id = "YDL243C" + out = task_align.exclude_gene_of_interest( + df, ensembl_id="", fname_full_gene="./tests/aad4.fasta", threads=2 + ) + assert aad4_gene_id not in out["gene_id"] + + +def test_join_alignment_with_annotation(task_align: AlignProbeCandidates): + task_align.fname_sam = "./tests/aad4_basic.sam" + task_align.fname_count = "./tests/count_table1.tsv" + task_align.fname_gtf = "./tests/sacCer3.gtf.parq" + df_sam = task_align.filter_unique_probes(is_endogenous=True) + df_norm = task_align.norm_table + + df = task_align.join_alignment_with_annotation(df_sam, df_norm) + assert len(df) >= len(df_sam) + for col in ["gene_id", "count", "qname"]: + assert col in df.columns + +def test_get_most_expressed_genes(task_align: AlignProbeCandidates): + task_align.fname_count = "./tests/count_table1.tsv" + task_align.fname_gtf = "./tests/sacCer3.gtf.parq" -def test_get_maximum_fpkm(task_align): - # task_align.get_maximum_fpkm(df_sam, df_counts, df_gtf) - pass + percentage10 = task_align.get_most_expressed_genes(task_align.norm_table, 10) + percentage90 = task_align.get_most_expressed_genes(task_align.norm_table, 90) + assert len(percentage10) >= len(percentage90) diff --git a/tests/test_integration.py b/tests/test_integration.py index 8789d9a..f2838f4 100644 --- a/tests/test_integration.py +++ b/tests/test_integration.py @@ -99,7 +99,7 @@ def test_passed_exogenous(): csv_files = glob.glob("./renilla_*.csv") assert len(csv_files) == 1 verify_csv(csv_files[0], args) - [os.remove(f) for f in csv_files] + [os.remove(f) for f in glob.glob("./renilla_*")] def test_downloaded_endogenous_optimal(): @@ -151,10 +151,10 @@ def test_counttable_full_output(): "./tests/sacCer3.gtf", "--ensembl-id", "YKL185W", - # "--encode-count-table", - # "./tests/.tsv", - "--max-off-target-fpkm", - "15", + "--encode-count-table", + "./tests/count_table1.tsv", + "--max-expression-percentage", + "40", "--debug", ] tic = time.time()